TTGE_PSEPT
ID TTGE_PSEPT Reviewed; 1046 AA.
AC Q9KWV4; E0X9D0; I7CDZ6;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2012, sequence version 2.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Toluene efflux pump membrane transporter TtgE;
GN Name=ttgE; Synonyms=sepb; OrderedLocusNames=T1E_4280;
OS Pseudomonas putida (strain DOT-T1E).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1196325;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=DOT-T1E;
RX PubMed=10648517; DOI=10.1128/jb.182.4.937-943.2000;
RA Mosqueda G., Ramos J.L.;
RT "A set of genes encoding a second toluene efflux system in Pseudomonas
RT putida DOT-T1 is linked to the tod genes for toluene metabolism.";
RL J. Bacteriol. 182:937-943(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=DOT-T1E;
RX PubMed=11251828; DOI=10.1046/j.1365-2958.2001.02310.x;
RA Duque E., Segura A., Mosqueda G., Ramos J.L.;
RT "Global and cognate regulators control the expression of the organic
RT solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida.";
RL Mol. Microbiol. 39:1100-1106(2001).
RN [3]
RP SEQUENCE REVISION.
RA Mosqueda G.;
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=DOT-T1E;
RA Daniels C., Godoy P., Duque E., Molina-Henares M.A., de la Torre J.,
RA Del Arco J.M., Herrera C., Segura A., Guazzaroni M.E., Ferrer M.,
RA Ramos J.L.;
RT "Global regulation of food supply by Pseudomonas putida DOT-T1E.";
RL Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DOT-T1E;
RX PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT DOT-T1E deduced from its annotated genome.";
RL Microb. Biotechnol. 6:598-611(2013).
CC -!- FUNCTION: The inner membrane transporter component of an inducible
CC organic solvent efflux pump. Involved in export of toluene and styrene
CC but not of m-xylene, propylbenzene or ethylbenzene. Is not involved in
CC antibiotic or AMP efflux.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: The ttgDEF operon is induced in the presence of toluene and
CC styrene, but not m-xylene. {ECO:0000269|PubMed:10648517,
CC ECO:0000269|PubMed:11251828}.
CC -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC (TC 2.A.6) family. {ECO:0000305}.
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DR EMBL; GQ884177; ADI95409.1; -; Genomic_DNA.
DR EMBL; CP003734; AFO50109.1; -; Genomic_DNA.
DR RefSeq; WP_012052588.1; NC_018220.1.
DR AlphaFoldDB; Q9KWV4; -.
DR SMR; Q9KWV4; -.
DR TCDB; 2.A.6.2.10; the resistance-nodulation-cell division (rnd) superfamily.
DR EnsemblBacteria; AFO50109; AFO50109; T1E_4280.
DR KEGG; ppx:T1E_4280; -.
DR PATRIC; fig|1196325.3.peg.4237; -.
DR HOGENOM; CLU_002755_0_1_6; -.
DR Proteomes; UP000006503; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR Gene3D; 3.30.2090.10; -; 2.
DR InterPro; IPR027463; AcrB_DN_DC_subdom.
DR InterPro; IPR001036; Acrflvin-R.
DR InterPro; IPR004764; HAE1.
DR PANTHER; PTHR32063; PTHR32063; 1.
DR Pfam; PF00873; ACR_tran; 1.
DR PRINTS; PR00702; ACRIFLAVINRP.
DR SUPFAM; SSF82714; SSF82714; 2.
DR TIGRFAMs; TIGR00915; 2A0602; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..1046
FT /note="Toluene efflux pump membrane transporter TtgE"
FT /id="PRO_0000161852"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 542..562
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 871..891
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 895..915
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 927..947
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 973..993
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1008..1028
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1046 AA; 113360 MW; 09C97337E6DA8904 CRC64;
MSRFFIDRPI FAWVLAIIAM LAGALSLTKM PISQYPNIAA PAVSIQVVYP GASAKTVQDT
VVQVIEQQLN GLDGFRYMAA ESASDGSMNI IVTFEQGTNP DIAQVQVQNK LQLATPRLPE
EVQRQGLRVV KYQMNFFMVV GLVDKTGKMT NFDLGNLIAS QLQDPISRIN GVGDFLLFGS
PYAMRIWLDP GKLNSYQLTP GDVAQAIREQ NVQVSSGQLG GLPTRSGVQL NATVVGKTRM
TTPAEFEEIL VKVKADGSQV RVKDLGRVVL ASENFAISAK YRGQDSAGLG LRLASGGNLL
ETVKAVKAEL EKQKAYLPEG VEVIYPYDTS PVVEASIDSV VHTILEAVVL VFLVMFLFLQ
SLRATIIPTL AVPVVLLAAF ALLPYFGISI NVLTMYAMVL AIGLLVDDAI VVVENVERLM
HDEGLSPLEA TRKSMGQISG ALVGIGMVLS AVFVPMAFFG GSAGIIYKQF AVTIVICMSL
SVLVALIFTP ALCATILKAP ENDAHHEKKG FFGWFNRSFD RNSARFERGV GGILKHRGRY
LLIFALITAG TGYLFTQIPK AFLPSEDQGL MMTEVRMPLN ASAERTEVVL QEVKDYLLKE
EGQLVDHVMT VNGFNFAGRG QNSGLVLVVL KDWAARQAAG EDVLSVAERA NARFARIKDA
TVMAFVPPAV LEMGNAMGFD LYLQDNLGLG HESLMAARNQ FLELAAENPS LRAVRPNGKD
DEPQFQVKID DEKARALQVS IASINDTMSA AWGSMYVNDF IDLGRVKRVY IQGVDSSRIA
PEDFDKWYVR NALGEMVPFS AFATGEWIHG SPKLERYGGI SAVNILGEPA PGFSTGDAMI
AIAQIMQQLP SGIGLSYNGL SYEEIRTGDQ APMLYALTVL IVFLCLAALY ESWSVPMSVI
LVVPLGIFGA VLATLWRGLE ADVYFQVGLM TTVGLSAKNA ILIIEFAKEL YEKEGVPLVK
AAIEAARLRL RPIIMTSLAF TFGVLPMARA TGAGAGSQHS IATGVVGGMI TATVLAVFFV
PLFYVVVVKV FERNKKPAAL AEEELA
