ID   TTGF_PSEPT              Reviewed;         480 AA.
AC   Q9KWV3; I7BEK2; Q3LWR5;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 2.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Toluene efflux pump outer membrane protein TtgF;
DE   Flags: Precursor;
GN   Name=ttgF; Synonyms=sepC; OrderedLocusNames=T1E_4279;
OS   Pseudomonas putida (strain DOT-T1E).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1196325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=10648517; DOI=10.1128/jb.182.4.937-943.2000;
RA   Mosqueda G., Ramos J.L.;
RT   "A set of genes encoding a second toluene efflux system in Pseudomonas
RT   putida DOT-T1 is linked to the tod genes for toluene metabolism.";
RL   J. Bacteriol. 182:937-943(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=11251828; DOI=10.1046/j.1365-2958.2001.02310.x;
RA   Duque E., Segura A., Mosqueda G., Ramos J.L.;
RT   "Global and cognate regulators control the expression of the organic
RT   solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida.";
RL   Mol. Microbiol. 39:1100-1106(2001).
RN   [3]
RP   SEQUENCE REVISION.
RA   Mosqueda G.;
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RC   STRAIN=DOT-T1E;
RA   Daniels C., Godoy P., Duque E., Molina-Henares M.A., de la Torre J.,
RA   Del Arco J.M., Herrera C., Segura A., Guazzaroni M.E., Ferrer M.,
RA   Ramos J.L.;
RT   "Global regulation of food supply by Pseudomonas putida DOT-T1E.";
RL   Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KL47;
RX   PubMed=16728956;
RA   Lee K., Ryu E.K., Choi K.S., Cho M.C., Jeong J.J., Choi E.N., Lee S.O.,
RA   Yoon D.Y., Hwang I., Kim C.K.;
RT   "Identification and expression of the cym, cmt, and tod catabolic genes
RT   from Pseudomonas putida KL47: expression of the regulatory todST genes as a
RT   factor for catabolic adaptation.";
RL   J. Microbiol. 44:192-199(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA   Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA   Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT   "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT   DOT-T1E deduced from its annotated genome.";
RL   Microb. Biotechnol. 6:598-611(2013).
CC   -!- FUNCTION: The outer membrane component of an inducible organic solvent
CC       efflux pump. Involved in export of toluene and styrene but not of m-
CC       xylene, propylbenzene or ethylbenzene. Is not involved in antibiotic or
CC       AMP efflux.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: The ttgDEF operon is induced in the presence of toluene and
CC       styrene, but not m-xylene. {ECO:0000269|PubMed:10648517,
CC       ECO:0000269|PubMed:11251828}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFO50108.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; GQ884177; ADI95408.1; -; Genomic_DNA.
DR   EMBL; DQ157469; ABA10820.1; -; Genomic_DNA.
DR   EMBL; CP003734; AFO50108.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_012052589.1; NC_018220.1.
DR   AlphaFoldDB; Q9KWV3; -.
DR   SMR; Q9KWV3; -.
DR   TCDB; 2.A.6.2.10; the resistance-nodulation-cell division (rnd) superfamily.
DR   EnsemblBacteria; AFO50108; AFO50108; T1E_4279.
DR   KEGG; ppx:T1E_4279; -.
DR   PATRIC; fig|1196325.3.peg.4236; -.
DR   HOGENOM; CLU_012817_13_3_6; -.
DR   Proteomes; UP000006503; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR003423; OMP_efflux.
DR   InterPro; IPR010131; RND_efflux_OM_lipoprot_NodT.
DR   Pfam; PF02321; OEP; 2.
DR   TIGRFAMs; TIGR01845; outer_NodT; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   2: Evidence at transcript level;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Signal;
KW   Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           17..480
FT                   /note="Toluene efflux pump outer membrane protein TtgF"
FT                   /id="PRO_0000031005"
FT   LIPID           17
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           17
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   480 AA;  52890 MW;  46E264EC2EE11352 CRC64;
     MKTHYLSIAL SVALSGCSLI PDYQRPPAPI QAGWPQGEAY AKLKAGTHRP SQTRDAELNW
     QVFFRDPVMR ELIATALNNN RDLRQTALNV EAYRALHRIE RSALLPRANT GVGATRQRLP
     ADLSPTGEAG IQSQYDTTLS MSYELDMFGR LRSLERAALQ EYLAAAETQR SMQIALIADV
     AIAYLSWRSD QAQLDLARST LASYENSLNL IKSSREVGTA SALDVRQARS LVETARVQQT
     LYTRQVAQDM NALQLLLGTK LPADLPISDV LDQPLAALST GLPADLLLHR PDIRAAEHRL
     LAANANIGAA RAAFFPSITL TAAAGTASHE LDGLFEGGSG LWAFMPRINL PIFTAGRLRG
     NLDYRNVIKD INVAEYEKSI QTAFREVADG LAARGTFGEQ LQAQRDLVDN NQAYYKLAYQ
     RYDEGVDNYL AVLDAQRELF AAQQQFLSDR LNQLSSEVRL FKALGGGWDN ISSQPLTAQN