ID   TTGG_PSEPT              Reviewed;         391 AA.
AC   Q93PU5;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Toluene efflux pump periplasmic linker protein TtgG;
DE   Flags: Precursor;
GN   Name=ttgG;
OS   Pseudomonas putida (strain DOT-T1E).
OG   Plasmid pGRT1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1196325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], EFFLUX PUMP SUBSTRATES, AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA   Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA   Segura A.;
RT   "Three efflux pumps are required to provide efficient tolerance to toluene
RT   in Pseudomonas putida DOT-T1E.";
RL   J. Bacteriol. 183:3967-3973(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP   221; 262 AND 321.
RC   STRAIN=DOT-T1E;
RX   PubMed=21605303; DOI=10.1111/j.1462-2920.2011.02492.x;
RA   Molina L., Duque E., Gomez M.J., Krell T., Lacal J., Garcia-Puente A.,
RA   Garcia V., Matilla M.A., Ramos J.L., Segura A.;
RT   "The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant
RT   for survival under harsh conditions in the environment.";
RL   Environ. Microbiol. 13:2315-2327(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA   Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA   Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT   "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT   DOT-T1E deduced from its annotated genome.";
RL   Microb. Biotechnol. 6:598-611(2013).
RN   [4]
RP   OPERON ORGANIZATION, AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=12896994; DOI=10.1128/jb.185.16.4755-4763.2003;
RA   Rojas A., Segura A., Guazzaroni M.E., Teran W., Hurtado A., Gallegos M.T.,
RA   Ramos J.L.;
RT   "In vivo and in vitro evidence that TtgV is the specific regulator of the
RT   TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.";
RL   J. Bacteriol. 185:4755-4763(2003).
CC   -!- FUNCTION: The periplasmic linker component of an organic solvent efflux
CC       pump. Involved in export of a number of organic solvents, including
CC       toluene and styrene. This is the most important solvent efflux pump in
CC       this strain, although it can export AMP and some antibiotics.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: Constitutively expressed; the ttgGHI operon is further
CC       induced about 4-fold by toluene and styrene but not by antibiotics.
CC       {ECO:0000269|PubMed:11395460, ECO:0000269|PubMed:12896994}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; HM626202; AAK69563.2; -; Genomic_DNA.
DR   RefSeq; WP_014003969.1; NC_015855.1.
DR   RefSeq; YP_004750623.1; NC_015855.1.
DR   AlphaFoldDB; Q93PU5; -.
DR   SMR; Q93PU5; -.
DR   TCDB; 2.A.6.2.11; the resistance-nodulation-cell division (rnd) superfamily.
DR   Proteomes; UP000006503; Plasmid pGRT1.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Coiled coil; Lipoprotein; Membrane;
KW   Palmitate; Plasmid; Signal; Transport.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           33..391
FT                   /note="Toluene efflux pump periplasmic linker protein TtgG"
FT                   /id="PRO_0000018720"
FT   COILED          107..136
FT                   /evidence="ECO:0000255"
FT   LIPID           33
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           33
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   391 AA;  42543 MW;  C7BA4637C3F48A14 CRC64;
     MRAERWSQTV RQIRSPRALR VIPLTALMLI SGCGEKEQVS SATPPPDVGV YTVRAQALTL
     TTDLPGRTSA FRVAEVRPQV SGILQKRSFV EGAEVKLGQQ LYQIDPRTYE AQLRRAEANR
     TSAQNLARRY ETLLKTKAVS KQQYDDALAA WKQAEADYQV ARIDVQYTRV LSPISGRIGR
     STVTEGALVT NGQAQSLATV TQLDPIYVDV TQPITKLLGL QKALESGRLQ KTGENQAEVS
     LTLDDGSAYP LPGTLKFSEV SVDPTTGSVT LRAEFPNPNR KLLPGMFVHA LLKEGVQNAA
     ILVPQQAISR DTRGVPSVWV VKADNTVESR EIQTLRTVGN AWLISNGVTE GERIITEGVQ
     RVRSGIAVNA VEAKNVNLVD GFAATTEASA N