TTGH_PSEPT
ID TTGH_PSEPT Reviewed; 1049 AA.
AC Q93PU4;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 2.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Toluene efflux pump membrane transporter TtgH;
GN Name=ttgH;
OS Pseudomonas putida (strain DOT-T1E).
OG Plasmid pGRT1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1196325;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=DOT-T1E;
RX PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA Segura A.;
RT "Three efflux pumps are required to provide efficient tolerance to toluene
RT in Pseudomonas putida DOT-T1E.";
RL J. Bacteriol. 183:3967-3973(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP 813; 838; 866-867 AND 883.
RC STRAIN=DOT-T1E;
RX PubMed=21605303; DOI=10.1111/j.1462-2920.2011.02492.x;
RA Molina L., Duque E., Gomez M.J., Krell T., Lacal J., Garcia-Puente A.,
RA Garcia V., Matilla M.A., Ramos J.L., Segura A.;
RT "The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant
RT for survival under harsh conditions in the environment.";
RL Environ. Microbiol. 13:2315-2327(2011).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DOT-T1E;
RX PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT DOT-T1E deduced from its annotated genome.";
RL Microb. Biotechnol. 6:598-611(2013).
RN [4]
RP OPERON ORGANIZATION, AND INDUCTION.
RC STRAIN=DOT-T1E;
RX PubMed=12896994; DOI=10.1128/jb.185.16.4755-4763.2003;
RA Rojas A., Segura A., Guazzaroni M.E., Teran W., Hurtado A., Gallegos M.T.,
RA Ramos J.L.;
RT "In vivo and in vitro evidence that TtgV is the specific regulator of the
RT TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.";
RL J. Bacteriol. 185:4755-4763(2003).
CC -!- FUNCTION: The inner membrane transporter component of an organic
CC solvent efflux pump. Involved in export of a number of organic
CC solvents, including toluene and styrene. This is the most important
CC solvent efflux pump in this strain, although it can export AMP and some
CC antibiotics.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: Constitutively expressed; the ttgGHI operon is further
CC induced about 4-fold by toluene and styrene but not by antibiotics.
CC {ECO:0000269|PubMed:11395460, ECO:0000269|PubMed:12896994}.
CC -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC (TC 2.A.6) family. {ECO:0000305}.
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DR EMBL; HM626202; AAK69564.2; -; Genomic_DNA.
DR RefSeq; WP_014003970.1; NC_015855.1.
DR RefSeq; YP_004750624.1; NC_015855.1.
DR AlphaFoldDB; Q93PU4; -.
DR SMR; Q93PU4; -.
DR TCDB; 2.A.6.2.11; the resistance-nodulation-cell division (rnd) superfamily.
DR Proteomes; UP000006503; Plasmid pGRT1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR Gene3D; 3.30.2090.10; -; 2.
DR InterPro; IPR027463; AcrB_DN_DC_subdom.
DR InterPro; IPR001036; Acrflvin-R.
DR InterPro; IPR004764; HAE1.
DR PANTHER; PTHR32063; PTHR32063; 1.
DR Pfam; PF00873; ACR_tran; 1.
DR PRINTS; PR00702; ACRIFLAVINRP.
DR SUPFAM; SSF82714; SSF82714; 2.
DR TIGRFAMs; TIGR00915; 2A0602; 1.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; Plasmid; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..1049
FT /note="Toluene efflux pump membrane transporter TtgH"
FT /id="PRO_0000161854"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 542..562
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 871..891
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 895..915
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 927..947
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 973..993
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1008..1028
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1049 AA; 114110 MW; A5A0782BE2BBCD81 CRC64;
MSRFFIDRPI FAWVLAIVAM LAGALSLAKM PISQYPNIAA PAVSIQVSYP GASAQTVQDT
VVQVIEQQLS GLDGFRYMSA ESASDGSMTI IVTFEQGTDP DIAQVQVQNK LQLATPRLPE
EVQRQGLRVV KYQMNFFLVM SLVDRSGKLD NFDLGNLIAS QLQDPISRIP GVGDFQLFGS
PYAMRIWLDP GKLNSYQLTP TDVASAIREQ NVQVSSGQLG GLPTRSGVQL NATVLGKTRM
TTPSQFDEIL VKVNPDGSQV RVKDVGRAEL GADSFAISAQ YKDSPTASLA LRLSTGGNLL
ETVDAVKKLM EQQKAYLPDG VEVIYPYDTT PVVEASIESV VHTIFEAVVL VFLVMYLFLQ
SFRATLIPTL AVPVVLLATF ALLPYFGLNI NVLTMYAMVL AIGLLVDDAI VVVENVERLM
HDEGLSPLEA TRKSMDQISG ALVGIGMVLS AVFVPMAFFG GSAGIIYQQF AITIVVCMGL
SILVALVFTP ALCVTILKAP EGNSHHERKG FFGWFNRIFD RGTRRFERGV GAMLKGRGRY
LLAFLLITGG TGYLFTQIPK AFLPNEDQGL MMIEVRTPAN ASAERTEGVL QEVRDYLAND
EGALVEHFMT VNGFNFAGRG QNSGLVLITF KDWKERHGAG QDVFSIAQRA NQHFAKIKDA
SVMAFVPPAI LEMGNAMGFN LYLQDNLGLG HEALMAARNQ FLQLASQNPK LQAVRPNGKD
DEPQFQVNID DEKARALQVS IASINETMSA AWGSMYVNDF IDRGRVKRVY VQGEDISRIS
PEDFDKWYVR NSLGQMVPFS AFATGEWVNG SPKLERYGGI SSLNILGEPA PGYSTGDAMI
AIAEIMQQLP AGIGLSYTGL SYEEIQTGDQ APLLYALTVL IVFLCLAALY ESWSVPVSVI
MVVPLGILGA VLATLWRDLT ADVYFQVGLM TTVGLSAKNA ILIVEFAKEL YEKEGYPIVK
AAIEAAKLRL RPILMTSLAF TFGVLPMAIA SGAGAGSQHS IATGVVGGMI TATVLAVFFV
PLFYVVVVKL FEGLMKRKPN AVKEVTHEV
