ID   TTGI_PSEPT              Reviewed;         470 AA.
AC   Q93PU3;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Toluene efflux pump outer membrane protein TtgI;
DE   Flags: Precursor;
GN   Name=ttgI;
OS   Pseudomonas putida (strain DOT-T1E).
OG   Plasmid pGRT1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1196325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA   Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA   Segura A.;
RT   "Three efflux pumps are required to provide efficient tolerance to toluene
RT   in Pseudomonas putida DOT-T1E.";
RL   J. Bacteriol. 183:3967-3973(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 289
RP   AND 378.
RC   STRAIN=DOT-T1E;
RX   PubMed=21605303; DOI=10.1111/j.1462-2920.2011.02492.x;
RA   Molina L., Duque E., Gomez M.J., Krell T., Lacal J., Garcia-Puente A.,
RA   Garcia V., Matilla M.A., Ramos J.L., Segura A.;
RT   "The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant
RT   for survival under harsh conditions in the environment.";
RL   Environ. Microbiol. 13:2315-2327(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA   Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA   Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT   "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT   DOT-T1E deduced from its annotated genome.";
RL   Microb. Biotechnol. 6:598-611(2013).
RN   [4]
RP   OPERON ORGANIZATION, AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=12896994; DOI=10.1128/jb.185.16.4755-4763.2003;
RA   Rojas A., Segura A., Guazzaroni M.E., Teran W., Hurtado A., Gallegos M.T.,
RA   Ramos J.L.;
RT   "In vivo and in vitro evidence that TtgV is the specific regulator of the
RT   TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.";
RL   J. Bacteriol. 185:4755-4763(2003).
CC   -!- FUNCTION: The outer membrane component of an organic solvent efflux
CC       pump. Involved in export of a number of organic solvents, including
CC       toluene and styrene. This is the most important solvent efflux pump in
CC       this strain, although it can export AMP and some antibiotics.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: Constitutively expressed; the ttgGHI operon is further
CC       induced about 4-fold by toluene and styrene but not by antibiotics.
CC       {ECO:0000269|PubMed:11395460, ECO:0000269|PubMed:12896994}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
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DR   EMBL; HM626202; AAK69565.2; -; Genomic_DNA.
DR   RefSeq; WP_014003971.1; NC_015855.1.
DR   RefSeq; YP_004750625.1; NC_015855.1.
DR   AlphaFoldDB; Q93PU3; -.
DR   SMR; Q93PU3; -.
DR   TCDB; 2.A.6.2.11; the resistance-nodulation-cell division (rnd) superfamily.
DR   Proteomes; UP000006503; Plasmid pGRT1.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR003423; OMP_efflux.
DR   InterPro; IPR010131; RND_efflux_OM_lipoprot_NodT.
DR   Pfam; PF02321; OEP; 2.
DR   TIGRFAMs; TIGR01845; outer_NodT; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   2: Evidence at transcript level;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal;
KW   Transmembrane; Transmembrane beta strand.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           17..470
FT                   /note="Toluene efflux pump outer membrane protein TtgI"
FT                   /id="PRO_0000031007"
FT   REGION          104..123
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        106..123
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           17
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           17
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   470 AA;  51387 MW;  C67F5C669E9308D6 CRC64;
     MKFKSLPMFA LLMLGGCSLI PDYQQPAAPM QAQWPTGQAY GGQGDQRSIA TALPKAKEFF
     KDPALVRLLD AALENNRDLR IAAKNVESYR ALYRIQRAER FPTLDGQASG NRTRLPDDLS
     PTGDSRIDSQ YQVGLVTAYE LDLFGRIRSL SNQALEKYLA TEEAQRSVQI ALIGDVATTY
     FLWRTDQALL ELTEATLTSY VESLAMIESS AWAGTSSELD VRQARTLVNQ AQAQQALYTR
     RIAQDVNALE LLLGSKIPTD LPKNSPLAMS ALGKVPAGLP ADLLLNRPDI RSAEHQLMAA
     NANIGAARAA FFPRISLTAS AGSASSDLDG LFNSGSDSWS FAPQISVPIF NAGKLRANLD
     YAELQKDVGV ATYEKSIQTA FREVADGLAA RGTYGKQLSA QSELVDNYKA YFSLAQQRYD
     QGVDSYLTVL DAQRELFSSQ QKLLNDQLDQ INSEVQLYKA LGGGWSVSQN