ID   TTGV_PSEPT              Reviewed;         259 AA.
AC   Q93PU6;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=HTH-type transcriptional regulator TtgV;
DE   AltName: Full=Toluene tolerance pump ttgGHI operon repressor;
GN   Name=ttgV;
OS   Pseudomonas putida (strain DOT-T1E).
OG   Plasmid pGRT1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1196325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA   Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA   Segura A.;
RT   "Three efflux pumps are required to provide efficient tolerance to toluene
RT   in Pseudomonas putida DOT-T1E.";
RL   J. Bacteriol. 183:3967-3973(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=21605303; DOI=10.1111/j.1462-2920.2011.02492.x;
RA   Molina L., Duque E., Gomez M.J., Krell T., Lacal J., Garcia-Puente A.,
RA   Garcia V., Matilla M.A., Ramos J.L., Segura A.;
RT   "The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant
RT   for survival under harsh conditions in the environment.";
RL   Environ. Microbiol. 13:2315-2327(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA   Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA   Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT   "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT   DOT-T1E deduced from its annotated genome.";
RL   Microb. Biotechnol. 6:598-611(2013).
RN   [4]
RP   OPERON ORGANIZATION, INDUCTION, AND CHARACTERIZATION.
RC   STRAIN=DOT-T1E;
RX   PubMed=12896994; DOI=10.1128/jb.185.16.4755-4763.2003;
RA   Rojas A., Segura A., Guazzaroni M.E., Teran W., Hurtado A., Gallegos M.T.,
RA   Ramos J.L.;
RT   "In vivo and in vitro evidence that TtgV is the specific regulator of the
RT   TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.";
RL   J. Bacteriol. 185:4755-4763(2003).
RN   [5]
RP   CHARACTERIZATION.
RC   STRAIN=DOT-T1E;
RX   PubMed=15126451; DOI=10.1128/jb.186.10.2921-2927.2004;
RA   Guazzaroni M.E., Teran W., Zhang X., Gallegos M.T., Ramos J.L.;
RT   "TtgV bound to a complex operator site represses transcription of the
RT   promoter for the multidrug and solvent extrusion TtgGHI pump.";
RL   J. Bacteriol. 186:2921-2927(2004).
CC   -!- FUNCTION: Represses the expression of the ttgGHI and ttgVW operons.
CC       Binds to the ttgGHI / ttgVW intergenic region, probably preventing
CC       binding of RNA polymerase; ttgV dissociates from this region in the
CC       presence of 1-hexanol.
CC   -!- INDUCTION: The ttgVW operon is induced by toluene and styrene, but not
CC       by the antibiotics carbenicillin, chloramphenicol, nalidixic acid,
CC       tetracycline or gentamicin. {ECO:0000269|PubMed:12896994}.
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DR   EMBL; HM626202; AAK69562.1; -; Genomic_DNA.
DR   RefSeq; WP_014003968.1; NC_015855.1.
DR   RefSeq; YP_004750622.1; NC_015855.1.
DR   PDB; 2XRN; X-ray; 2.90 A; A/B=14-253.
DR   PDB; 2XRO; X-ray; 3.40 A; A/B/E/F=14-253.
DR   PDBsum; 2XRN; -.
DR   PDBsum; 2XRO; -.
DR   AlphaFoldDB; Q93PU6; -.
DR   SMR; Q93PU6; -.
DR   EvolutionaryTrace; Q93PU6; -.
DR   Proteomes; UP000006503; Plasmid pGRT1.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.40; -; 1.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR014757; Tscrpt_reg_IclR_C.
DR   InterPro; IPR005471; Tscrpt_reg_IclR_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF09339; HTH_IclR; 1.
DR   Pfam; PF01614; IclR; 1.
DR   SMART; SM00346; HTH_ICLR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51077; HTH_ICLR; 1.
DR   PROSITE; PS51078; ICLR_ED; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..259
FT                   /note="HTH-type transcriptional regulator TtgV"
FT                   /id="PRO_0000201767"
FT   DOMAIN          14..76
FT                   /note="HTH iclR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT   DOMAIN          89..253
FT                   /note="IclR-ED"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00394"
FT   DNA_BIND        36..59
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT   HELIX           16..28
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           36..42
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           47..58
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   TURN            59..61
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          62..65
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           67..69
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          71..74
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           77..104
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          106..114
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          117..124
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          128..130
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          139..141
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           142..144
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           146..154
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           157..163
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          172..174
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           178..191
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          193..201
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          204..213
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   STRAND          216..225
FT                   /evidence="ECO:0007829|PDB:2XRN"
FT   HELIX           226..251
FT                   /evidence="ECO:0007829|PDB:2XRN"
SQ   SEQUENCE   259 AA;  27411 MW;  1B2A580D89A85767 CRC64;
     MNQSDENIGK AGGIQVIARA ASIMRALGSH PHGLSLAAIA QLVGLPRSTV QRIINALEEE
     FLVEALGPAG GFRLGPALGQ LINQAQTDIL SLVKPYLRSL AEELDESVCL ASLAGDKIYV
     LDRIVSEREL RVVFPIGINV PAAATAAGKV LLAALPDETL QAALGEQLPV LTSNTLGRKA
     LVKQLSEVRQ SGVASDLDEH IDGVCSFATL LDTYLGYYSL AIVMPSSRAS KQSDLIKKAL
     LQSKLNIERA IGRASKKAP