ID   TTGW_PSEPT              Reviewed;         134 AA.
AC   Q93PU7;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Uncharacterized HTH-type transcriptional regulator TtgW;
GN   Name=ttgW;
OS   Pseudomonas putida (strain DOT-T1E).
OG   Plasmid pGRT1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1196325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA   Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA   Segura A.;
RT   "Three efflux pumps are required to provide efficient tolerance to toluene
RT   in Pseudomonas putida DOT-T1E.";
RL   J. Bacteriol. 183:3967-3973(2001).
RN   [2]
RP   SEQUENCE REVISION.
RC   STRAIN=DOT-T1E;
RA   Rojas A.M., Segura A., Ramos J.L.;
RL   Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=21605303; DOI=10.1111/j.1462-2920.2011.02492.x;
RA   Molina L., Duque E., Gomez M.J., Krell T., Lacal J., Garcia-Puente A.,
RA   Garcia V., Matilla M.A., Ramos J.L., Segura A.;
RT   "The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant
RT   for survival under harsh conditions in the environment.";
RL   Environ. Microbiol. 13:2315-2327(2011).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA   Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA   Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT   "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT   DOT-T1E deduced from its annotated genome.";
RL   Microb. Biotechnol. 6:598-611(2013).
RN   [5]
RP   OPERON ORGANIZATION, INDUCTION, AND CHARACTERIZATION.
RC   STRAIN=DOT-T1E;
RX   PubMed=12896994; DOI=10.1128/jb.185.16.4755-4763.2003;
RA   Rojas A., Segura A., Guazzaroni M.E., Teran W., Hurtado A., Gallegos M.T.,
RA   Ramos J.L.;
RT   "In vivo and in vitro evidence that TtgV is the specific regulator of the
RT   TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.";
RL   J. Bacteriol. 185:4755-4763(2003).
CC   -!- FUNCTION: Unknown, does not seem to be involved in regulation of the
CC       ttgGHI or ttgVW operons.
CC   -!- INDUCTION: The ttgVW operon is induced by toluene and styrene, but not
CC       by the antibiotics carbenicillin, chloramphenicol, nalidixic acid,
CC       tetracycline or gentamicin. {ECO:0000269|PubMed:12896994}.
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DR   EMBL; HM626202; AAK69561.2; -; Genomic_DNA.
DR   RefSeq; WP_014003967.1; NC_015855.1.
DR   RefSeq; YP_004750621.1; NC_015855.1.
DR   AlphaFoldDB; Q93PU7; -.
DR   SMR; Q93PU7; -.
DR   Proteomes; UP000006503; Plasmid pGRT1.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR023772; DNA-bd_HTH_TetR-type_CS.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS01081; HTH_TETR_1; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Plasmid; Transcription; Transcription regulation.
FT   CHAIN           1..134
FT                   /note="Uncharacterized HTH-type transcriptional regulator
FT                   TtgW"
FT                   /id="PRO_0000070626"
FT   DOMAIN          10..70
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        33..52
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ   SEQUENCE   134 AA;  15030 MW;  69F70FDAF7DE8E4B CRC64;
     MARKTAAEAK ETRQRIIDAA LEVFVAQGVP DATLDQIARK AGVTRGAVYW HFNGKLEVLQ
     AVLASRQHPL ELDFTPDLGI ERSWEAVVVA MLDAVHSPQS KQFSEILIYQ GLDESGLIHN
     RMVQASDRFL QYIR