ID   C513E_DICDI             Reviewed;         504 AA.
AC   Q54SN0;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Probable cytochrome P450 513E1;
DE            EC=1.14.-.-;
GN   Name=cyp513E1; ORFNames=DDB_G0282353;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AAFI02000047; EAL66298.1; -; Genomic_DNA.
DR   RefSeq; XP_640271.1; XM_635179.1.
DR   AlphaFoldDB; Q54SN0; -.
DR   SMR; Q54SN0; -.
DR   STRING; 44689.DDB0232342; -.
DR   PaxDb; Q54SN0; -.
DR   PRIDE; Q54SN0; -.
DR   EnsemblProtists; EAL66298; EAL66298; DDB_G0282353.
DR   GeneID; 8623532; -.
DR   KEGG; ddi:DDB_G0282353; -.
DR   dictyBase; DDB_G0282353; cyp513E1.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_22_0_1; -.
DR   InParanoid; Q54SN0; -.
DR   OMA; EQEYMPF; -.
DR   PhylomeDB; Q54SN0; -.
DR   PRO; PR:Q54SN0; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..504
FT                   /note="Probable cytochrome P450 513E1"
FT                   /id="PRO_0000318818"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         450
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   504 AA;  57923 MW;  297317C892FB2C12 CRC64;
     MNLYISILIL IISLIIFFKN NNRISKINSK IPGPIRLPIF GNLLQINKDP HIQFQKWYEK
     YGVIYSIRLG NIETVVFTGY PIFKKAFIEN SQIFAPRFQH LSRFEANGCK NLIGSNDEIH
     STLKKLILTE ITSNKIKKME NHIVLECENL CKQLDKHCQD GLPFSLNMYF KLFSLNIILR
     FLFGTINNSY QDKSNQDIVD VIIEFLHYGG NPIMSDFIPI LKPFYKQNKF FKFYPILCDH
     LNKLIENYKN NKQLQKQQKQ QQNEDDDDDD DGTIIGKLLK EYHNGKISWT SVVSTCVDVF
     LAGVDSTSNS TIFTLIALVN NSNCQEKLFN EIKNNLKKSD DGHDEIVIRH SLYRSSIPYL
     SLVMKEVYRL YSVILIGLPH ITSEDVEIEG YKIAKGTQII QNVFSTHLCE KTFPMSKSFI
     PERFIETGSN NMFGGGQTNL VHFGTGVRDC VGKSLADCEI FTVLATLINR YQFINPTLEP
     LNDIGSFGIA YQPPINNFII KKRL