ID   TTH_ACIAM               Reviewed;         535 AA.
AC   G8YXZ9;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   22-FEB-2012, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Tetrathionate hydrolase {ECO:0000303|PubMed:21747790};
DE            Short=4THase {ECO:0000250|UniProtKB:B7J3C9};
DE            Short=TTH {ECO:0000303|PubMed:21747790};
DE            EC=3.12.1.- {ECO:0000269|PubMed:21747790};
DE   Flags: Precursor;
GN   Name=tth1 {ECO:0000303|PubMed:21747790};
OS   Acidianus ambivalens (Desulfurolobus ambivalens).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Acidianus.
OX   NCBI_TaxID=2283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-53, FUNCTION,
RP   CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR
RP   LOCATION, INDUCTION, AND GLYCOSYLATION AT ASN-50.
RC   STRAIN=Lei 10 / DSM 3772 / JCM 9191;
RX   PubMed=21747790; DOI=10.3389/fmicb.2011.00068;
RA   Protze J., Mueller F., Lauber K., Nass B., Mentele R., Lottspeich F.,
RA   Kletzin A.;
RT   "An extracellular tetrathionate hydrolase from the thermoacidophilic
RT   archaeon Acidianus ambivalens with an activity optimum at pH 1.";
RL   Front. Microbiol. 2:68-68(2011).
CC   -!- FUNCTION: Catalyzes the hydrolysis of tetrathionate to generate
CC       elemental sulfur, thiosulfate and sulfate.
CC       {ECO:0000269|PubMed:21747790}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + tetrathionate = H(+) + sulfate + sulfur + thiosulfate;
CC         Xref=Rhea:RHEA:13541, ChEBI:CHEBI:15226, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:26833,
CC         ChEBI:CHEBI:33542; Evidence={ECO:0000269|PubMed:21747790};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.8 mM for tetrathionate (at pH 1 and 80 degrees Celsius)
CC         {ECO:0000269|PubMed:21747790};
CC       pH dependence:
CC         Optimum pH is 1. No activity is detected at pH 6.
CC         {ECO:0000269|PubMed:21747790};
CC       Temperature dependence:
CC         Optimum temperature is 95 degrees Celsius.
CC         {ECO:0000269|PubMed:21747790};
CC   -!- SUBUNIT: Monomer and homodimer; in equilibrium.
CC       {ECO:0000269|PubMed:21747790}.
CC   -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000269|PubMed:21747790}.
CC       Note=Associated with the S-layer in the pseudo-periplasmic space.
CC       {ECO:0000269|PubMed:21747790}.
CC   -!- INDUCTION: Expressed in tetrathionate-grown cells.
CC       {ECO:0000269|PubMed:21747790}.
CC   -!- SIMILARITY: Belongs to the tetrathionate hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; FR734215; CBY66038.1; -; Genomic_DNA.
DR   AlphaFoldDB; G8YXZ9; -.
DR   SMR; G8YXZ9; -.
DR   iPTMnet; G8YXZ9; -.
DR   BRENDA; 3.12.1.B1; 86.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR018391; PQQ_beta_propeller_repeat.
DR   InterPro; IPR002372; PQQ_repeat.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   Pfam; PF13360; PQQ_2; 1.
DR   SMART; SM00564; PQQ; 5.
DR   SUPFAM; SSF50998; SSF50998; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Hydrolase; Signal.
FT   SIGNAL          1..39
FT                   /evidence="ECO:0000269|PubMed:21747790"
FT   CHAIN           40..535
FT                   /note="Tetrathionate hydrolase"
FT                   /id="PRO_0000444002"
FT   CARBOHYD        50
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:21747790"
SQ   SEQUENCE   535 AA;  57659 MW;  F3E798AE92B19E82 CRC64;
     MNLKILVGLF ILGIIILSAM TFLNFTTIVA QDKGDQQPKG PIVYTYTEYN GTYFPHILTV
     VYYPCNATCS NLGFPSVWPV SNENQCHNAV VNTTCQALIE GVDWQLPLLN YVGGVAIPLS
     TPPCKLPWAQ QLGVKGALVY YTQMVGEPLG VTLACGLLYA TEDSMPGSIT AINPVTGKIV
     WMANGLAGPA MNNPVVWHGI VYVSVGGVCF TFSQFVHFED HQYCKIHRGR CGAVYAFNAT
     NGQLLWMRFT YGEAMPAPAI YDGILAYVTG GGCFVGVNAT TGQTLWVDHF PGFIANMASV
     NYYVLPNGTP LFIAGFTYTA PPYGYIIAVN GLNGHEAWNA TMPAPYVGAN TGLGDVAPAV
     DQQLGIVVDN DIANFSNGYV DMVTFALNAT NGKPLWAVNT GRGPIPPAYK GGMPLIVGNV
     VYDGNPSLGT VNAICIKTGK ILWSTKLPCL QTPPKFPGGP RGSPTYYHGL LWVSAGQYVY
     VINPKNGDIL TFYWIGGRLG IMNPVIAGNT MFLSNSYGWI VAIPLSQIYP AYIYY