ID   C5141_DICDI             Reviewed;         575 AA.
AC   Q54FM9;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Probable cytochrome P450 514A1;
DE            EC=1.14.-.-;
GN   Name=cyp514A1; ORFNames=DDB_G0290743;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AAFI02000169; EAL62077.1; -; Genomic_DNA.
DR   RefSeq; XP_635583.1; XM_630491.1.
DR   AlphaFoldDB; Q54FM9; -.
DR   SMR; Q54FM9; -.
DR   STRING; 44689.DDB0232982; -.
DR   PaxDb; Q54FM9; -.
DR   EnsemblProtists; EAL62077; EAL62077; DDB_G0290743.
DR   GeneID; 8627804; -.
DR   KEGG; ddi:DDB_G0290743; -.
DR   dictyBase; DDB_G0290743; cyp514A1.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_22_0_1; -.
DR   InParanoid; Q54FM9; -.
DR   OMA; TIWDLFS; -.
DR   PhylomeDB; Q54FM9; -.
DR   PRO; PR:Q54FM9; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..575
FT                   /note="Probable cytochrome P450 514A1"
FT                   /id="PRO_0000318820"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         448
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   575 AA;  66214 MW;  B3833EC23A3FADA6 CRC64;
     MNTIFTIILT ITILVLSLIL KDLLFEGRIK KINKLIPGPS TIPVFGNLLQ INAKDFPKSV
     NDFYERYGKV FRLRLGSVEI VVLTGPEVID ECFNKKHREI FKERYIKFSR FFGKDYNIIS
     SNGDYHYVLR GILTSEITTR KLNNGRLESN KFILEMFSNL CKDNKETLVK NTPNQIRILA
     VKLILNFTLG IEENDETILI IVEKIKCIFE AAGLLIYSDY LPFLFPLDIK SMSKNDIISS
     YFFLKDFIGI KLDAIKIKYE KENELKNETT DETSSKLNNI PIIENYYKNY LDGSIHYDSI
     LFSISDIIFA AVDSTSNGFS LLIGQLINKP EIQDKIYEEI MRNDENNNTN NISFADHTKY
     PYIISVMNES YRYNSSVPIT EPNKTTEDVE VNGYKIAKGT MIIKNLRGTH LSKEFWGEDA
     LEFKPERFKN QPLYQKGLFH FGAGPRGCPG GRFTESLTFT FLVIMLKNFK IVNPTDIPID
     VEGEVGLAMQ CKPFDALFIK HTNRNDFSKI NKGLNQQCFT SSSQFPTCYQ TEFFEFGFNG
     NFQLNDGIDC SSGVQQLSFF EKGFVFRVDH FLFEF