ID   TTI_GLOMM               Reviewed;          53 AA.
AC   O97373;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Tsetse thrombin inhibitor;
DE   Flags: Precursor;
GN   Name=TTI;
OS   Glossina morsitans morsitans (Savannah tsetse fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Hippoboscoidea;
OC   Glossinidae; Glossina.
OX   NCBI_TaxID=37546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Salivary gland;
RX   PubMed=9826693; DOI=10.1073/pnas.95.24.14290;
RA   Cappello M., Li S., Chen X., Li C.-B., Harrison L., Narashimhan S.,
RA   Beard C.B., Aksoy S.;
RT   "Tsetse thrombin inhibitor: bloodmeal-induced expression of an
RT   anticoagulant in salivary glands and gut tissue of Glossina morsitans
RT   morsitans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:14290-14295(1998).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-42, FUNCTION, TISSUE SPECIFICITY, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Salivary gland;
RX   PubMed=8644901; DOI=10.4269/ajtmh.1996.54.475;
RA   Cappello M., Bergum P.W., Vlasuk G.P., Furmidge B.A., Pritchard D.I.,
RA   Aksoy S.;
RT   "Isolation and characterization of the tsetse thrombin inhibitor: a potent
RT   antithrombotic peptide from the saliva of Glossina morsitans morsitans.";
RL   Am. J. Trop. Med. Hyg. 54:475-480(1996).
CC   -!- FUNCTION: Potent and specific inhibitor of human thrombin. It is also a
CC       potent inhibitor of thrombin-induced platelet aggregation. It is
CC       capable of antagonizing host hemostasis and facilitating blood feeding.
CC       {ECO:0000269|PubMed:8644901}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed at high levels in salivary glands and
CC       midguts of adult tsetse flies. {ECO:0000269|PubMed:8644901,
CC       ECO:0000269|PubMed:9826693}.
CC   -!- MASS SPECTROMETRY: Mass=3530; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:8644901};
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DR   EMBL; AF054616; AAC79986.1; -; mRNA.
DR   PDB; 6TKG; X-ray; 1.35 A; I=1-53.
DR   PDB; 6TKH; X-ray; 1.90 A; I=1-53.
DR   PDB; 6TKI; X-ray; 1.80 A; I=1-53.
DR   PDB; 6TKJ; X-ray; 2.81 A; I=1-53.
DR   PDB; 6TKL; X-ray; 1.30 A; I=1-53.
DR   PDB; 7PHX; X-ray; 1.80 A; I=22-53.
DR   PDBsum; 6TKG; -.
DR   PDBsum; 6TKH; -.
DR   PDBsum; 6TKI; -.
DR   PDBsum; 6TKJ; -.
DR   PDBsum; 6TKL; -.
DR   PDBsum; 7PHX; -.
DR   AlphaFoldDB; O97373; -.
DR   SMR; O97373; -.
DR   MEROPS; I76.001; -.
DR   Proteomes; UP000092444; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..53
FT                   /note="Tsetse thrombin inhibitor"
FT                   /id="PRO_5000054168"
FT   STRAND          24..26
FT                   /evidence="ECO:0007829|PDB:6TKL"
FT   HELIX           45..47
FT                   /evidence="ECO:0007829|PDB:6TKL"
SQ   SEQUENCE   53 AA;  5800 MW;  7A37FC9AA57B4239 CRC64;
     MKFFTVLFFL LSIIYLIVAA PGEPGAPIDY DEYGDSSEEV GGTPLHEIPG IRL