ID   C5142_DICDI             Reviewed;         500 AA.
AC   Q1ZXC4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Probable cytochrome P450 514A2;
DE            EC=1.14.-.-;
GN   Name=cyp514A2; ORFNames=DDB_G0294561;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AAFI02000162; EAS66829.1; -; Genomic_DNA.
DR   RefSeq; XP_001134512.1; XM_001134512.1.
DR   AlphaFoldDB; Q1ZXC4; -.
DR   SMR; Q1ZXC4; -.
DR   STRING; 44689.DDB0232984; -.
DR   PaxDb; Q1ZXC4; -.
DR   EnsemblProtists; EAS66829; EAS66829; DDB_G0294561.
DR   GeneID; 8627579; -.
DR   KEGG; ddi:DDB_G0294561; -.
DR   dictyBase; DDB_G0294561; cyp514A2.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_22_0_1; -.
DR   InParanoid; Q1ZXC4; -.
DR   OMA; HTLAPMG; -.
DR   PhylomeDB; Q1ZXC4; -.
DR   PRO; PR:Q1ZXC4; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..500
FT                   /note="Probable cytochrome P450 514A2"
FT                   /id="PRO_0000318821"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         446
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   500 AA;  57276 MW;  AD7788B41EB6D580 CRC64;
     MNLIYTIILT IIILVLIISI KDLFFEDKIK KINKSIPSPP TIPIFGNLLQ INSKDVATCF
     NDFYKQYGKV YRLRLGSVET VVLTGGDIID ECFNKKYRDF LKARYVKFSR YLGKDTNILH
     SNGDYHFLLK GVLSSQVTVR KLNNGRLEFN KYILQMFNNL NNNDEGSTMF LANDVPSQIK
     KLILKVVLNF TLGIEENDDI NLSLFQNGSN IFKAAGLFIY SDYLPFLFPL DIKSMAKSNM
     ISSYVFVRDY LAKKLEEVKK KEYIINGDDD GGVDTSQTPL IESYYKLYLQ GLIGYDSILL
     SIVDIIIASV DTTSNSISFI IARLTNHQEI QSKIYEEIMS NDINNNSNNI SFSDHSKYPY
     IISIMNETYR YYASVPLPEP NMTTEDIEVD GYKIAKGTQI YKNIRGTLIS KEFWGEDALE
     FKPERFKTQT LNQKGLLHFG AGPRGCPGAR FTECFFFTLM VLLFKNYKLQ NPNDNPIDDR
     GDVGLSMQCK PYDALFIKRN