ID   C5144_DICDI             Reviewed;         502 AA.
AC   Q54FP9;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Probable cytochrome P450 514A4;
DE            EC=1.14.-.-;
GN   Name=cyp514A4; ORFNames=DDB_G0290707;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AAFI02000167; EAL62088.1; -; Genomic_DNA.
DR   RefSeq; XP_635592.1; XM_630500.1.
DR   AlphaFoldDB; Q54FP9; -.
DR   SMR; Q54FP9; -.
DR   STRING; 44689.DDB0232979; -.
DR   PaxDb; Q54FP9; -.
DR   EnsemblProtists; EAL62088; EAL62088; DDB_G0290707.
DR   GeneID; 8627789; -.
DR   KEGG; ddi:DDB_G0290707; -.
DR   dictyBase; DDB_G0290707; cyp514A4.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_22_0_1; -.
DR   InParanoid; Q54FP9; -.
DR   OMA; LMARILW; -.
DR   PhylomeDB; Q54FP9; -.
DR   PRO; PR:Q54FP9; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..502
FT                   /note="Probable cytochrome P450 514A4"
FT                   /id="PRO_0000318822"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         448
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   502 AA;  57704 MW;  4474FB776A36F1AE CRC64;
     MNTIFTIILT ITILVLSLIL KDLLFEGRIK KINKLIPGPS TIPVFGNLLQ INAKDFPKSV
     NDFYERYGKV FRLRLGSVEI VVLTGPEVID ECFNKKHREI FKERYIKFSR FLGKDYNIFS
     SNGDYHYVLR GILTSEITTR KLNNGRLESN KFILEMFSNL CKDNKETLVK NTPNQIRILA
     VKLILNFTLG IEENDETILI IVEKIKCIFE AAGLLIYSDY LPFLFPLDIK SMSKNDIISS
     YFFIKDFIGI KLDAIKIKYE KENELKNETT DETSSKLNNI PIIENYYKNY LDGSIHYDSI
     LFSISDIIFA AVDSTSNGFS LLIGQLINKP EIQDKIYEEI MRNDENNNTN NISFADHTKY
     PYIISVMNES YRYNSSVPIT EPNKTTEDVE VNGYKIAKGT MIIKNLRGTH LSKEFWGEDA
     LEFKPERFKN QPLNQKGLFH FGAGARACPG GRFTESFTFT FLVIMLKNFK IVNPTDIPID
     VEGEVGLAMQ CKPFDALFIK RN