ID   C516A_DICDI             Reviewed;         487 AA.
AC   Q54DT2;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Probable cytochrome P450 516A1;
DE            EC=1.14.-.-;
GN   Name=cyp516A1; ORFNames=DDB_G0292168;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AAFI02000187; EAL61440.2; -; Genomic_DNA.
DR   RefSeq; XP_629790.2; XM_629788.2.
DR   AlphaFoldDB; Q54DT2; -.
DR   SMR; Q54DT2; -.
DR   STRING; 44689.DDB0232988; -.
DR   PaxDb; Q54DT2; -.
DR   EnsemblProtists; EAL61440; EAL61440; DDB_G0292168.
DR   GeneID; 8628467; -.
DR   KEGG; ddi:DDB_G0292168; -.
DR   dictyBase; DDB_G0292168; cyp516A1.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_22_0_1; -.
DR   InParanoid; Q54DT2; -.
DR   OMA; AEINQTG; -.
DR   PhylomeDB; Q54DT2; -.
DR   Reactome; R-DDI-211935; Fatty acids.
DR   Reactome; R-DDI-211958; Miscellaneous substrates.
DR   Reactome; R-DDI-211981; Xenobiotics.
DR   Reactome; R-DDI-211999; CYP2E1 reactions.
DR   Reactome; R-DDI-2142670; Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
DR   Reactome; R-DDI-2142816; Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
DR   Reactome; R-DDI-5423646; Aflatoxin activation and detoxification.
DR   Reactome; R-DDI-9027307; Biosynthesis of maresin-like SPMs.
DR   Reactome; R-DDI-9749641; Aspirin ADME.
DR   Reactome; R-DDI-9753281; Paracetamol ADME.
DR   PRO; PR:Q54DT2; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0020037; F:heme binding; IBA:GO_Central.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016712; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR   GO; GO:0006082; P:organic acid metabolic process; IBA:GO_Central.
DR   GO; GO:0006805; P:xenobiotic metabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..487
FT                   /note="Probable cytochrome P450 516A1"
FT                   /id="PRO_0000318825"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         434
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   487 AA;  55281 MW;  9483BADB1CF78BAC CRC64;
     MIILLLSIII FILYIVKIFK NKSCCGNEII LPPGPISLPF IGNLHQLAID PHLAIQKLMF
     KYGNVMTVYF ANIKTVVISD PNYLKEVFVN QSHKTSDRYL MGTSRIIGNE KDILFSNGQY
     WKNYRQILAQ SFLKLRDHKG ITEKISLESV KLSQAFEWYA TSGQVVNPCS LFKMYTLNVI
     MQLLYSHRSS YDLKGQHPII DALKMVEESL AVGNVLDLFP ILNIFLKKNK LKLVNNLQQV
     WSYSQDSIKE HREKLLINPE KIDDLLDLFI NEIKLSKNSE FFDDEGLYRV CSDLLLSGTE
     TSSSTMSWLL LFLINNPNFQ DKVRTELLEA TGGKKTIGLT EKSKTPFFNA CIKEALRIRP
     VGALSLPRIA SEDVTCGPYT IEKGSQIIMN VYGLAMDPTV WEDPETFNPY RWLSSDISQS
     TYSFIPFGCG SRVCVGSSLA RDEIFLGIGN ILLNYIFESQ NGKPINEKGH FGIALQTVDY
     NVKLTKI