TTL_ARATH
ID TTL_ARATH Reviewed; 421 AA.
AC F4JRR5; Q8L3Y4; Q9SW36;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=TITAN-like protein;
GN Name=TTL; OrderedLocusNames=At4g24900; ORFNames=F13M23.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, ALTERNATIVE SPLICING, DISRUPTION PHENOTYPE, ZINC-FINGER, TISSUE
RP SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=22991160; DOI=10.1093/jxb/ers243;
RA Lu X., Li Y., Su Y., Liang Q., Meng H., Li S., Shen S., Fan Y., Zhang C.;
RT "An Arabidopsis gene encoding a C2H2-domain protein with alternatively
RT spliced transcripts is essential for endosperm development.";
RL J. Exp. Bot. 63:5935-5944(2012).
CC -!- FUNCTION: Key regulator for endosperm and embryo nuclear divisions.
CC {ECO:0000269|PubMed:22991160}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22991160}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=F4JRR5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4JRR5-2; Sequence=VSP_053215, VSP_053216;
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons, stems, veins of sepals and
CC stigmas, and actively dividing tissues such as shoot apical meristem,
CC root tip and emerging trus leaves. Weak expression in petals and
CC anthers, and not detected in mature leaves. In seeds, expressed in both
CC the endosperm and embryo. {ECO:0000269|PubMed:22991160}.
CC -!- DOMAIN: The C-terminus is essential for the function of the protein and
CC it contains two conserved motifs (289-300) and (365-389) of unknown
CC function present in orthologous proteins from various eukaryotes.
CC {ECO:0000269|PubMed:22991160}.
CC -!- DISRUPTION PHENOTYPE: Aborted seed development. Embryos arrested at the
CC globular to heart stage transition and defective endosperm development
CC with multi-nucleolated endosperm cells. {ECO:0000269|PubMed:22991160}.
CC -!- MISCELLANEOUS: At least nine differentially spliced transcript isoforms
CC are produced. Full-length isoforms, but not the truncated ones lacking
CC the C-terminal domain, are able to rescue disruption mutants
CC (PubMed:22991160). {ECO:0000305|PubMed:22991160}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36732.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79399.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035523; CAB36732.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161562; CAB79399.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84974.1; -; Genomic_DNA.
DR EMBL; AY099687; AAM20538.1; -; mRNA.
DR EMBL; AY128869; AAM91269.1; -; mRNA.
DR PIR; T05511; T05511.
DR RefSeq; NP_194220.2; NM_118622.6. [F4JRR5-1]
DR AlphaFoldDB; F4JRR5; -.
DR BioGRID; 13880; 5.
DR IntAct; F4JRR5; 5.
DR STRING; 3702.AT4G24900.1; -.
DR iPTMnet; F4JRR5; -.
DR PaxDb; F4JRR5; -.
DR PRIDE; F4JRR5; -.
DR ProteomicsDB; 234654; -. [F4JRR5-1]
DR EnsemblPlants; AT4G24900.1; AT4G24900.1; AT4G24900. [F4JRR5-1]
DR GeneID; 828592; -.
DR Gramene; AT4G24900.1; AT4G24900.1; AT4G24900. [F4JRR5-1]
DR KEGG; ath:AT4G24900; -.
DR Araport; AT4G24900; -.
DR TAIR; locus:2117363; AT4G24900.
DR eggNOG; ENOG502QS8B; Eukaryota.
DR HOGENOM; CLU_041370_0_0_1; -.
DR InParanoid; F4JRR5; -.
DR OMA; YFPNHKK; -.
DR OrthoDB; 1213548at2759; -.
DR PRO; PR:F4JRR5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JRR5; baseline and differential.
DR Genevisible; F4JRR5; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009960; P:endosperm development; IMP:TAIR.
DR InterPro; IPR028015; CCDC84-like.
DR PANTHER; PTHR31198; PTHR31198; 1.
DR Pfam; PF14968; CCDC84; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Zinc; Zinc-finger.
FT CHAIN 1..421
FT /note="TITAN-like protein"
FT /id="PRO_0000423620"
FT ZN_FING 11..32
FT /note="C2H2-type 1; degenerate"
FT ZN_FING 70..100
FT /note="C2H2-type 2; degenerate"
FT REGION 279..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 376..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 376..397
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..215
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_053215"
FT VAR_SEQ 254..256
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_053216"
SQ SEQUENCE 421 AA; 47520 MW; 916C3745D1A5B8F1 CRC64;
MKKPSKKSEI EFCTVCRFHH DQGSRHKYFP RHKSSLSSLL DRFRSKIADV RFFLKNPSVL
RPQEQSQNRV WCVFCDEDIV ELGSSFACSK AINHFASSDH LKNIKQFLSK NGPAMDCIDE
FRISEADVAK WEKKCQSFGN EDASFEGSCG QLSGTSNDIH TKLAFETMDR IKKVPAHHIN
SYKSNDVMPL QYNTNEYQIS LSEIPGVIHN GSYLNMDDSQ FPLCDESGNG FGEHSIPCRS
KDYSGNGNYC TQENYQVSQD KKQIDGSYNP PGVVGMTSIS SSHSTDAGGN VHSGAPPPWL
DANDGDFSSV QLNQSDVARF QAKVPGKNRK LNPNRVGAAW AERRKIEIEM EKSGHVTKSN
IDPDWLPNFG RVWQSGTRKE SRKEFEKEKR KLVKTESIST ESEPVKIQPY ISKRARRESG
E
