TTL_SCHPO
ID TTL_SCHPO Reviewed; 403 AA.
AC Q10438;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Probable tubulin--tyrosine ligase C12B10.04;
DE EC=6.3.2.25;
GN ORFNames=SPAC12B10.04;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Probable tubulin--tyrosine ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-
CC [tubulin] + L-tyrosine = ADP + C-terminal L-alpha-aminoacyl-L-
CC glutamyl-L-glutamyl-L-tyrosyl-[tubulin] + H(+) + phosphate;
CC Xref=Rhea:RHEA:17605, Rhea:RHEA-COMP:16434, Rhea:RHEA-COMP:16435,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:58315, ChEBI:CHEBI:149554, ChEBI:CHEBI:149555,
CC ChEBI:CHEBI:456216; EC=6.3.2.25;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- COFACTOR:
CC Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the tubulin--tyrosine ligase family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAA94694.1; -; Genomic_DNA.
DR PIR; T37571; T37571.
DR RefSeq; NP_594636.1; NM_001020064.2.
DR AlphaFoldDB; Q10438; -.
DR SMR; Q10438; -.
DR BioGRID; 279365; 31.
DR STRING; 4896.SPAC12B10.04.1; -.
DR iPTMnet; Q10438; -.
DR MaxQB; Q10438; -.
DR PaxDb; Q10438; -.
DR EnsemblFungi; SPAC12B10.04.1; SPAC12B10.04.1:pep; SPAC12B10.04.
DR GeneID; 2542924; -.
DR KEGG; spo:SPAC12B10.04; -.
DR PomBase; SPAC12B10.04; -.
DR VEuPathDB; FungiDB:SPAC12B10.04; -.
DR eggNOG; KOG2157; Eukaryota.
DR HOGENOM; CLU_031301_0_0_1; -.
DR InParanoid; Q10438; -.
DR OMA; CSYVIRK; -.
DR PhylomeDB; Q10438; -.
DR PRO; PR:Q10438; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000932; C:P-body; ISO:PomBase.
DR GO; GO:0004835; F:tubulin-tyrosine ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006397; P:mRNA processing; ISO:PomBase.
DR GO; GO:0110154; P:RNA decapping; ISO:PomBase.
DR InterPro; IPR027746; TTL.
DR InterPro; IPR004344; TTL/TTLL_fam.
DR PANTHER; PTHR47551; PTHR47551; 1.
DR Pfam; PF03133; TTL; 1.
DR PROSITE; PS51221; TTL; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Ligase; Magnesium; Nucleus; Potassium; Reference proteome.
FT CHAIN 1..403
FT /note="Probable tubulin--tyrosine ligase C12B10.04"
FT /id="PRO_0000212447"
FT DOMAIN 9..386
FT /note="TTL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00568"
SQ SEQUENCE 403 AA; 46524 MW; B122F71756A0B108 CRC64;
MSSTKKDIKV YVNYRDEYAE PKIISALKNS GKELTFTNSA KEANFQWAQY EDIDFDEVYK
NPKTKLCCSY VIRKALIRKE YLWRTVITYL AKHPDSILSK SVPEAYSLEL DYAEFLDDSL
MEAYELRQEL EENATKNISE KQWYILKPSM CDRAQGIRLF STIEELQAIF DSFDDEESES
EEAGLEEKGD ITVAFNNKIV ISQIRNFLVQ KYISKPLLLD HRKFHIRAYV LATGALSVYL
FNEMLCLLAR DKYKKPTPDP DLLFSHLSNT CLQGDNVEQS SIRDFWNTSI ENKDDIFKSI
LNIIGDVFEA AATTQGIHFQ PLENCFEIFG VDFLVDCESQ VYLLEVNSYP DFKQTGKNLS
NIIENLFSAV VETAIIPFFE SSTKRNVDSK LTLAKKLQLF GFR
