TTTP_LAMBD
ID TTTP_LAMBD Reviewed; 131 AA.
AC P03732;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 02-JUN-2021, entry version 88.
DE RecName: Full=Tail tube terminator protein {ECO:0000305};
DE Short=TrP;
DE AltName: Full=Gene product U;
DE Short=gpU;
DE AltName: Full=Minor tail protein U;
DE AltName: Full=Tail sheath-stabilizing protein {ECO:0000305};
DE AltName: Full=Tail-to-head joining protein;
DE Short=THJP;
GN Name=U; OrderedLocusNames=lambdap12;
OS Escherichia phage lambda (Bacteriophage lambda).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Lambdavirus.
OX NCBI_TaxID=10710;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT "Nucleotide sequence of bacteriophage lambda DNA.";
RL J. Mol. Biol. 162:729-773(1982).
RN [2]
RP CHARACTERIZATION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=835226; DOI=10.1016/0042-6822(77)90290-2;
RA Katsura I., Tsugita A.;
RT "Purification and characterization of the major protein and the terminator
RT protein of the bacteriophage lambda tail.";
RL Virology 76:129-145(1977).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=2150582; DOI=10.1016/0065-227x(90)90004-d;
RA Katsura I.;
RT "Mechanism of length determination in bacteriophage lambda tails.";
RL Adv. Biophys. 26:1-18(1990).
RN [4]
RP STRUCTURE BY NMR.
RX PubMed=17056065; DOI=10.1016/j.jmb.2006.09.068;
RA Edmonds L., Liu A., Kwan J.J., Avanessy A., Caracoglia M., Yang I.,
RA Maxwell K.L., Rubenstein J., Davidson A.R., Donaldson L.W.;
RT "The NMR structure of the gpU tail-terminator protein from bacteriophage
RT lambda: identification of sites contributing to Mg(II)-mediated
RT oligomerization and biological function.";
RL J. Mol. Biol. 365:175-186(2007).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), AND SUBUNIT.
RX PubMed=19426744; DOI=10.1016/j.jmb.2009.04.072;
RA Pell L.G., Liu A., Edmonds L., Donaldson L.W., Howell P.L., Davidson A.R.;
RT "The X-ray crystal structure of the phage lambda tail terminator protein
RT reveals the biologically relevant hexameric ring structure and demonstrates
RT a conserved mechanism of tail termination among diverse long-tailed
RT phages.";
RL J. Mol. Biol. 389:938-951(2009).
CC -!- FUNCTION: Plays an essential role in tail assembly by capping the
CC rapidly polymerizing tail once it has reached its requisite length and
CC serving as the interaction surface for the completion protein.
CC {ECO:0000269|PubMed:2150582}.
CC -!- SUBUNIT: Homohexamer. May bind to major tail protein V, and /or tape
CC measure protein. {ECO:0000269|PubMed:19426744,
CC ECO:0000269|PubMed:835226}.
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm.
CC -!- SIMILARITY: Belongs to the lambda-like tail terminator protein family.
CC {ECO:0000305}.
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DR EMBL; J02459; AAA96544.1; -; Genomic_DNA.
DR PIR; C43008; TLBPUL.
DR RefSeq; NP_040591.1; NC_001416.1.
DR PDB; 1Z1Z; NMR; -; A=1-131.
DR PDB; 3FZ2; X-ray; 2.70 A; A/B/C/D/E/F/G/H/I/J/K/L=1-131.
DR PDB; 3FZB; X-ray; 2.80 A; A/B/C/D/E/F/G/H/I/J=1-131.
DR PDBsum; 1Z1Z; -.
DR PDBsum; 3FZ2; -.
DR PDBsum; 3FZB; -.
DR BMRB; P03732; -.
DR SMR; P03732; -.
DR IntAct; P03732; 7.
DR GeneID; 2703486; -.
DR KEGG; vg:2703486; -.
DR EvolutionaryTrace; P03732; -.
DR Proteomes; UP000001711; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0098015; C:virus tail; IDA:UniProtKB.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1700; -; 1.
DR InterPro; IPR038512; GpU-like_sf.
DR InterPro; IPR009312; Phage_lambda_GpU-like.
DR InterPro; IPR035934; Phage_tail_protein-like_sf.
DR Pfam; PF06141; Phage_tail_U; 1.
DR SUPFAM; SSF143749; SSF143749; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host cytoplasm; Late protein; Reference proteome;
KW Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Viral release from host cell;
KW Viral tail assembly; Viral tail protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..131
FT /note="Tail tube terminator protein"
FT /id="PRO_0000077670"
FT HELIX 2..17
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 20..26
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 29..31
FT /evidence="ECO:0007829|PDB:1Z1Z"
FT TURN 33..35
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 37..50
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 57..69
FT /evidence="ECO:0007829|PDB:3FZ2"
FT HELIX 74..84
FT /evidence="ECO:0007829|PDB:3FZ2"
FT HELIX 86..89
FT /evidence="ECO:0007829|PDB:3FZ2"
FT HELIX 93..98
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 100..111
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 113..115
FT /evidence="ECO:0007829|PDB:3FZ2"
FT STRAND 118..130
FT /evidence="ECO:0007829|PDB:3FZ2"
SQ SEQUENCE 131 AA; 14649 MW; 9E3BBE413C2D8E50 CRC64;
MKHTELRAAV LDALEKHDTG ATFFDGRPAV FDEADFPAVA VYLTGAEYTG EELDSDTWQA
ELHIEVFLPA QVPDSELDAW MESRIYPVMS DIPALSDLIT SMVASGYDYR RDDDAGLWSS
ADLTYVITYE M
