TTY2_DROME
ID TTY2_DROME Reviewed; 655 AA.
AC Q9W5A5; A8WHI0; O97175; Q8IRY1; Q8IRY2; Q8IRY3; Q9U1L5;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Protein tweety-2;
DE Short=Dttyh2;
GN ORFNames=CG3638;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=16219661; DOI=10.1113/expphysiol.2005.031773;
RA Suzuki M.;
RT "The Drosophila tweety family: molecular candidates for large-conductance
RT Ca2+-activated Cl- channels.";
RL Exp. Physiol. 91:141-147(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-597 AND SER-599, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Probable chloride channel. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000312|FlyBase:FBgn0261444};
CC IsoId=Q9W5A5-1; Sequence=Displayed;
CC Name=B {ECO:0000312|FlyBase:FBgn0261444};
CC IsoId=Q9W5A5-2; Sequence=VSP_029776;
CC -!- DISRUPTION PHENOTYPE: Death during development.
CC {ECO:0000269|PubMed:16219661}.
CC -!- SIMILARITY: Belongs to the tweety family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN09036.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB65886.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF45597.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09036.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AE014298; AAN09038.1; -; Genomic_DNA.
DR EMBL; AL121806; CAB65886.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BT031126; ABX00748.1; -; mRNA.
DR RefSeq; NP_001245460.1; NM_001258531.2. [Q9W5A5-2]
DR RefSeq; NP_726720.3; NM_166872.3.
DR RefSeq; NP_726721.1; NM_166873.3. [Q9W5A5-2]
DR RefSeq; NP_726722.1; NM_166874.3. [Q9W5A5-1]
DR AlphaFoldDB; Q9W5A5; -.
DR SMR; Q9W5A5; -.
DR BioGRID; 57634; 2.
DR STRING; 7227.FBpp0070239; -.
DR TCDB; 1.A.48.1.5; the anion channel tweety (tweety) family.
DR GlyGen; Q9W5A5; 7 sites.
DR iPTMnet; Q9W5A5; -.
DR PaxDb; Q9W5A5; -.
DR DNASU; 31071; -.
DR EnsemblMetazoa; FBtr0070249; FBpp0070239; FBgn0261444. [Q9W5A5-2]
DR EnsemblMetazoa; FBtr0308707; FBpp0300913; FBgn0261444. [Q9W5A5-2]
DR EnsemblMetazoa; FBtr0336978; FBpp0307920; FBgn0261444. [Q9W5A5-1]
DR GeneID; 31071; -.
DR KEGG; dme:Dmel_CG3638; -.
DR UCSC; CG3638-RA; d. melanogaster. [Q9W5A5-1]
DR FlyBase; FBgn0261444; CG3638.
DR VEuPathDB; VectorBase:FBgn0261444; -.
DR eggNOG; KOG4433; Eukaryota.
DR GeneTree; ENSGT00950000183060; -.
DR InParanoid; Q9W5A5; -.
DR PhylomeDB; Q9W5A5; -.
DR Reactome; R-DME-2672351; Stimuli-sensing channels.
DR BioGRID-ORCS; 31071; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 31071; -.
DR PRO; PR:Q9W5A5; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0261444; Expressed in wing disc and 22 other tissues.
DR ExpressionAtlas; Q9W5A5; baseline and differential.
DR Genevisible; Q9W5A5; DM.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:FlyBase.
DR GO; GO:0005254; F:chloride channel activity; ISS:UniProtKB.
DR GO; GO:0005229; F:intracellular calcium activated chloride channel activity; IBA:GO_Central.
DR GO; GO:0072320; F:volume-sensitive chloride channel activity; IBA:GO_Central.
DR GO; GO:0006821; P:chloride transport; ISS:FlyBase.
DR CDD; cd07912; Tweety_N; 1.
DR InterPro; IPR006990; Tweety.
DR PANTHER; PTHR12424; PTHR12424; 1.
DR Pfam; PF04906; Tweety; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Chloride; Chloride channel;
KW Glycoprotein; Ion channel; Ion transport; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..655
FT /note="Protein tweety-2"
FT /id="PRO_0000312257"
FT TOPO_DOM 1..54
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..95
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 117..231
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..258
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..401
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 423..655
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 446..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..488
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 521..576
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 597
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 599
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CARBOHYD 18
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 188
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 326
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 372
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 38..53
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_029776"
SQ SEQUENCE 655 AA; 71406 MW; 5422371D52542D94 CRC64;
MEQPSILVKI LHSIPHVNYT FRRVNDTFNP DSDVYLEPTN NKLQCQQKGI ELQSLVILAS
IPAGLLIGSL LGLLLYLLTR CCDRRQRKPS AQRCQSCSLV IITLMTCAAI GLGLYGNDDF
HNGLLQAFGS GKSVEGLVLT LKRQTESIKH DLETRMAGHK VEQLVEKPHY NQTVVMLLIE
TSRLVTENTS RAVASLDSMV HYFMKAKGSE NDTSLMGLLQ LGEFYESIRW PATLAFLTVL
LLLCTVLVIG VARRSRCTLI FFSVSGLFCI IICWLLAGVY LASSVAAGDF CMRPHDYMCR
QVGMRSPYVD FLNCGTPRNR FILRLNESRD LVDRARESVE HMQRMSQETY PHIDIQRTLN
AMDNDLEITL RNLTTLSATL DRRTIDMHYE EALRGLCGGG LLGLSLMMVA GLLTSFLLTI
LVYADSHAWI YLTKRPTLDA DKSETAPLFP ASNAPSASIS PTAPLSTGTI NRTLLHHQQA
SSGGGSGTLP GSGGGAGAGG GVGANGHNGV GPYRNGSAGL RQGLASPSSQ SSHTSSTATY
NNGTTSYHNS HQQHNNHLYS NHYSHSNNHY NNTQHRTNPA AGAVAAAVGV VSSGQRSPSP
PPVYDLVHGT RSGHHTLGRL PSHHQQSATY LPGPNNGKYA TLSKQCKTLE SNDFY
