ID   C520A_DICDI             Reviewed;         536 AA.
AC   Q54D38;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Probable cytochrome P450 520A1;
DE            EC=1.14.-.-;
GN   Name=cyp520A1; ORFNames=DDB_G0292496;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AAFI02000190; EAL61227.1; -; Genomic_DNA.
DR   RefSeq; XP_629658.1; XM_629656.1.
DR   AlphaFoldDB; Q54D38; -.
DR   SMR; Q54D38; -.
DR   STRING; 44689.DDB0233029; -.
DR   PaxDb; Q54D38; -.
DR   PRIDE; Q54D38; -.
DR   EnsemblProtists; EAL61227; EAL61227; DDB_G0292496.
DR   GeneID; 8628722; -.
DR   KEGG; ddi:DDB_G0292496; -.
DR   dictyBase; DDB_G0292496; cyp520A1.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_0_1; -.
DR   InParanoid; Q54D38; -.
DR   OMA; MRPKPYQ; -.
DR   PhylomeDB; Q54D38; -.
DR   PRO; PR:Q54D38; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IBA:GO_Central.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0008395; F:steroid hydroxylase activity; IBA:GO_Central.
DR   GO; GO:0006694; P:steroid biosynthetic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..536
FT                   /note="Probable cytochrome P450 520A1"
FT                   /id="PRO_0000318837"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         479
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   536 AA;  62136 MW;  DB57357B15EA94D8 CRC64;
     MEILTFIIYL ITFFILFDFY KKNKRYSKSP NKEANGPWSL PIIGGLHLIG DRPNRSFSEL
     SKIYGGIYKI WLAERMLMIV TDPEIIQDIW IKQHDKFVNR PHNITSQIFS LNHKSLVFGD
     VDEWNKVRPK MTCHFTKIKL NSTKPKQIVN DQLKKMLKIM TTHSLDSKPF NQYVYLNTYS
     MNIILGLMLS IELPHSNSND KDGQFSKVLH SIDEIFKSIG TNGPEDIFPT LLPFFKNRIS
     TFTNHLNVIK DFIRSIYKQQ IKTFDINIEP RNIMDCLISE YYEDDDQEDE VAKQELIIQL
     CIDMLVAATD TSASTLEWFM LFMINNPNLQ EDLYEEVVNV VGKDCPYVTF DDVPKLALIK
     ACYFEILRIR PVTSLSLPRV SMEDTTTLND IFIPKDTIII QNIFGMGNSE KFVSNPTVFN
     PSRWLEYKKM KDLNQFGNRD DSIDTTNTTT NTTLNGTTSK YYNDLERVSI PFGVGKRRCM
     APSMADHNVL IAMANIVLNF TMKSSDPKQM PLSEEEQYAI TIKPKYPFKV LFEKRS