ID   C520B_DICDI             Reviewed;         481 AA.
AC   Q54E98;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Putative cytochrome P450 520B1;
DE            EC=1.14.-.-;
GN   Name=cyp520B1; ORFNames=DDB_G0291702;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
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DR   EMBL; AAFI02000181; EAL61587.1; -; Genomic_DNA.
DR   RefSeq; XP_630004.1; XM_630002.1.
DR   AlphaFoldDB; Q54E98; -.
DR   SMR; Q54E98; -.
DR   STRING; 44689.DDB0252692; -.
DR   PaxDb; Q54E98; -.
DR   EnsemblProtists; EAL61587; EAL61587; DDB_G0291702.
DR   GeneID; 8628294; -.
DR   KEGG; ddi:DDB_G0291702; -.
DR   dictyBase; DDB_G0291702; cyp520B1.
DR   eggNOG; KOG0156; Eukaryota.
DR   HOGENOM; CLU_001570_4_0_1; -.
DR   InParanoid; Q54E98; -.
DR   OMA; ICKKLAT; -.
DR   PhylomeDB; Q54E98; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0020037; F:heme binding; IBA:GO_Central.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0008395; F:steroid hydroxylase activity; IBA:GO_Central.
DR   GO; GO:0006694; P:steroid biosynthetic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   5: Uncertain;
KW   Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..481
FT                   /note="Putative cytochrome P450 520B1"
FT                   /id="PRO_0000318838"
FT   BINDING         427
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   481 AA;  55857 MW;  C7BC1A6539B6FC33 CRC64;
     MTNNKLLFKK DVNGPWSLPI IGGIYLINDN PNRALTKLSK KYGGIYKIWL GESFSMVVSD
     PEIVNEIWVK QHDNFINRPK NITHKMFSSN YRSLNFGDNP NWKFNRSMAS SHFTKTKLLS
     SKVTSVVEKK LNKLIETMEY HSINKLPFDS YVGFSEYSLN IILNMLVSMD IDECENSTQN
     VIYSINEIFK MLSTNSPQYS FPYLKFFFKK DLNNFKFHLD KIKSFIHSIY LKQLESYDPS
     NPRNILDSFI SDLQSNDIDI LLQICIDIVV AGTDTVANLL QWFVLFCINY PEIQEKLYNE
     IIEVVGKDCK VLKYEHISKM PYLYGCFRES LRIRPVTPLS LPRVAKCDTY IKDDIFIPKG
     ATIIQNIFGM GNDEKYISEP NKFKPERWVE YIKNKKVNKN GNENSVNKYF NDLDKISIPF
     GVGKRQCLSP AMAEQESLLS IATVVLNYKL KSNGQKKLNE KEVYSITIKP QPFKLFLEKR
     V