TUBE_BPSPP
ID TUBE_BPSPP Reviewed; 264 AA.
AC O48449;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2016, sequence version 2.
DT 29-SEP-2021, entry version 72.
DE RecName: Full=Tail tube protein gp17.1* {ECO:0000303|PubMed:17611601};
DE Short=TTP {ECO:0000305};
DE AltName: Full=Gene product 17.1 {ECO:0000305};
DE Short=gp17.1 {ECO:0000305};
DE AltName: Full=Major tail protein {ECO:0000303|PubMed:18786146};
DE Short=MTP {ECO:0000303|PubMed:18786146};
GN Name=17.1;
OS Bacillus phage SPP1 (Bacteriophage SPP1).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae.
OX NCBI_TaxID=10724;
OH NCBI_TaxID=1423; Bacillus subtilis.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM TAIL TUBE PROTEIN GP17.1).
RX PubMed=9434185; DOI=10.1016/s0378-1119(97)00547-7;
RA Alonso J.C., Luder G., Stiege A.C., Chai S., Weise F., Trautner T.A.;
RT "The complete nucleotide sequence and functional organization of Bacillus
RT subtilis bacteriophage SPP1.";
RL Gene 204:201-212(1997).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17611601; DOI=10.1038/sj.emboj.7601786;
RA Plisson C., White H.E., Auzat I., Zafarani A., Sao-Jose C., Lhuillier S.,
RA Tavares P., Orlova E.V.;
RT "Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection.";
RL EMBO J. 26:3720-3728(2007).
RN [3]
RP PROTEIN SEQUENCE OF 1-8, FUNCTION, AND RIBOSOMAL FRAMESHIFT.
RX PubMed=18786146; DOI=10.1111/j.1365-2958.2008.06435.x;
RA Auzat I., Droege A., Weise F., Lurz R., Tavares P.;
RT "Origin and function of the two major tail proteins of bacteriophage
RT SPP1.";
RL Mol. Microbiol. 70:557-569(2008).
RN [4]
RP SUBUNIT.
RX PubMed=25525268; DOI=10.1074/jbc.m114.613166;
RA Langlois C., Ramboarina S., Cukkemane A., Auzat I., Chagot B., Gilquin B.,
RA Ignatiou A., Petitpas I., Kasotakis E., Paternostre M., White H.E.,
RA Orlova E.V., Baldus M., Tavares P., Zinn-Justin S.;
RT "Bacteriophage SPP1 tail tube protein self-assembles into beta-structure-
RT rich tubes.";
RL J. Biol. Chem. 290:3836-3849(2015).
RN [5] {ECO:0007744|PDB:5A20, ECO:0007744|PDB:5A21}
RP STRUCTURE BY ELECTRON MICROSCOPY (7.20 ANGSTROMS) OF 1-177, FUNCTION, AND
RP INTERACTION WITH THE TAIL COMPLETION PROTEIN GP17.
RX PubMed=25991862; DOI=10.1073/pnas.1504039112;
RA Chaban Y., Lurz R., Brasiles S., Cornilleau C., Karreman M.,
RA Zinn-Justin S., Tavares P., Orlova E.V.;
RT "Structural rearrangements in the phage head-to-tail interface during
RT assembly and infection.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:7009-7014(2015).
CC -!- FUNCTION: Forms the 160 nm long, sixfold helical, rigid tail tube.
CC Binding to the entry receptor triggers structural rearrangements of the
CC tail tube leading to ejection of the phage DNA into the host.
CC {ECO:0000269|PubMed:17611601, ECO:0000269|PubMed:18786146,
CC ECO:0000269|PubMed:25525268, ECO:0000269|PubMed:25991862}.
CC -!- SUBUNIT: Homohexamer (PubMed:25525268). Self-assembles into a long
CC sixfold helical tube (PubMed:25525268). SPP1 virion tails are composed
CC of about 40 stacked rings, and each tail ring is rotated by about 21
CC degrees relative to the previous one (PubMed:25525268). Interacts with
CC the tail completion protein gp17 (PubMed:25991862).
CC {ECO:0000269|PubMed:25525268, ECO:0000269|PubMed:25991862}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:17611601}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Ribosomal frameshifting; Named isoforms=2;
CC Name=Tail tube protein gp17.1*; Synonyms=Large tail tube protein;
CC IsoId=O48449-1; Sequence=Displayed;
CC Name=Tail tube protein gp17.1; Synonyms=Small tail tube protein;
CC IsoId=O48449-2; Sequence=VSP_058620;
CC -!- PTM: The tail tube is formed by the proteins gp17.1 and gp17.1* at a
CC ratio of about 3:1. {ECO:0000269|PubMed:18786146}.
CC -!- MISCELLANEOUS: [Isoform Tail tube protein gp17.1*]: Produced by
CC ribosomal frameshifting. {ECO:0000269|PubMed:18786146}.
CC -!- MISCELLANEOUS: [Isoform Tail tube protein gp17.1]: Produced by
CC conventional translation. {ECO:0000269|PubMed:18786146}.
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DR EMBL; X97918; CAA66550.1; -; Genomic_DNA.
DR PIR; T42289; T42289.
DR RefSeq; NP_690680.1; NC_004166.2. [O48449-2]
DR RefSeq; YP_710298.1; NC_004166.2. [O48449-1]
DR PDB; 5A20; EM; 7.60 A; H=1-177.
DR PDB; 5A21; EM; 7.20 A; H=1-177.
DR PDB; 6YEG; Other; 4.00 A; A/B/C/D/E/F/G/H/I/J/K/L=5-177.
DR PDB; 6YQ5; Other; 4.00 A; A/B/C/D/E/F/G/H/I/J/K/L=5-175.
DR PDBsum; 5A20; -.
DR PDBsum; 5A21; -.
DR PDBsum; 6YEG; -.
DR PDBsum; 6YQ5; -.
DR SMR; O48449; -.
DR GeneID; 4229189; -.
DR GeneID; 955320; -.
DR KEGG; vg:4229189; -.
DR KEGG; vg:955320; -.
DR Proteomes; UP000002559; Genome.
DR GO; GO:0098026; C:virus tail, tube; IDA:UniProtKB.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR GO; GO:0098005; P:viral head-tail joining; IDA:CACAO.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR011855; Phgtail_TP901_1.
DR Pfam; PF00041; fn3; 1.
DR Pfam; PF06199; Phage_tail_2; 1.
DR SMART; SM00060; FN3; 1.
DR SUPFAM; SSF49265; SSF49265; 1.
DR TIGRFAMs; TIGR02126; phgtail_TP901_1; 1.
DR PROSITE; PS50853; FN3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Ribosomal frameshifting; Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Viral tail protein;
KW Viral tail tube protein; Virion; Virus entry into host cell.
FT CHAIN 1..264
FT /note="Tail tube protein gp17.1*"
FT /id="PRO_0000408481"
FT DOMAIN 179..261
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT VAR_SEQ 176..264
FT /note="LPNAPQNLTYTATTDSVTVKWDAVEGADSYNVYRGAEKKLDANVTTTSHTLT
FT GIQPDTQLTVNVAAVNAGGESPMSQIVTKTLPAESTG -> AP (in isoform
FT Tail tube protein gp17.1)"
FT /id="VSP_058620"
SQ SEQUENCE 264 AA; 28267 MW; C8FEFFB528C154D7 CRC64;
MPETPIMGQD VKYLFQSIDA ATGSAPLFPA YQTDGSVSGE RELFDEQTKN GRILGPGSVA
DSGEVTYYGK RGDAGQKAIE DAYQNGKQIK FWRVDTVKNE NDKYDAQFGF AYIESREYSD
GVEGAVEISI SLQVIGELKN GEIDTLPEEI VNVSKGGYDF QQPGQTTGEA PGTVPLPNAP
QNLTYTATTD SVTVKWDAVE GADSYNVYRG AEKKLDANVT TTSHTLTGIQ PDTQLTVNVA
AVNAGGESPM SQIVTKTLPA ESTG
