ID   C525A_DICDI             Reviewed;         601 AA.
AC   Q7KWN2; Q1ZXL9;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Probable cytochrome P450 525A1;
DE            EC=1.14.-.-;
GN   Name=cyp525A1; ORFNames=DDB_G0272652;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AAFI02000008; EAS66966.1; -; Genomic_DNA.
DR   RefSeq; XP_001134632.1; XM_001134632.1.
DR   AlphaFoldDB; Q7KWN2; -.
DR   SMR; Q7KWN2; -.
DR   STRING; 44689.DDB0233046; -.
DR   PaxDb; Q7KWN2; -.
DR   EnsemblProtists; EAS66966; EAS66966; DDB_G0272652.
DR   GeneID; 8618676; -.
DR   KEGG; ddi:DDB_G0272652; -.
DR   dictyBase; DDB_G0272652; cyp525A1.
DR   eggNOG; KOG0157; Eukaryota.
DR   HOGENOM; CLU_454508_0_0_1; -.
DR   InParanoid; Q7KWN2; -.
DR   OMA; CEEPHWL; -.
DR   PhylomeDB; Q7KWN2; -.
DR   PRO; PR:Q7KWN2; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..601
FT                   /note="Probable cytochrome P450 525A1"
FT                   /id="PRO_0000318842"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          205..253
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         544
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   601 AA;  70174 MW;  A6F2B46F54EFA211 CRC64;
     MDTTKNNDEF DISYFLTCSI FGFILWILTE QILKYYNKTN KNNKYNLPKG PSFLKWFINY
     LFNFYDLKLS NNKEEDNNNN NNKSNNSLSQ EELIEDTSEN TVLKWFNQLN SDNYSVSFFG
     RPMIFTRDTT ISKYILSSNN IDNYTKPPDS SGVLIRLAQN SILMSEGDQW RYHRSIINQP
     FSSKNVKLMI PTIITTINKL INHLNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
     NNNNNNNNNN NNNNNNNNTI IIDIHSYCTK LTFDIIGKLS IGYDFNSIES SDNDNDNNDD
     DDISKQFDFI LNEMIRPIRR FSSYLPLYND IKLFKFLNEL ESIIKGAINS RSLITDNNNN
     KTYKKNFLLD NLLDDNVKEK DIIGNINTFL LAGHETSANL LTFIFYLLST HNNVQNDLYN
     HLIENQKKKI NKDNKFTEED EDYQSIEFLD WVIYETLRLF PPAPMIGRTS KNDDILKSGN
     NNNNNNNNIS IPSETLILIS VYAIHRDPKL WKDPNIFNPY RWKNIENINN RSDFIPFSSG
     GRVCVGQKFS IVEARIIISK LILNFELSFN NLKSKPFKIY QRATLTPKYP VFLNFKKREN
     K