TUBE_LAMBD
ID TUBE_LAMBD Reviewed; 246 AA.
AC P03733;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 02-JUN-2021, entry version 92.
DE RecName: Full=Tail tube protein;
DE Short=TTP;
DE AltName: Full=Gene product V;
DE Short=gpV;
DE AltName: Full=Major tail protein V;
GN Name=V; OrderedLocusNames=lambdap13;
OS Escherichia phage lambda (Bacteriophage lambda).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Lambdavirus.
OX NCBI_TaxID=10710;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT "Nucleotide sequence of bacteriophage lambda DNA.";
RL J. Mol. Biol. 162:729-773(1982).
RN [2]
RP CHARACTERIZATION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=835226; DOI=10.1016/0042-6822(77)90290-2;
RA Katsura I., Tsugita A.;
RT "Purification and characterization of the major protein and the terminator
RT protein of the bacteriophage lambda tail.";
RL Virology 76:129-145(1977).
RN [3]
RP FUNCTION.
RX PubMed=2150582; DOI=10.1016/0065-227x(90)90004-d;
RA Katsura I.;
RT "Mechanism of length determination in bacteriophage lambda tails.";
RL Adv. Biophys. 26:1-18(1990).
RN [4]
RP FUNCTION, AND INTERACTION WITH GPGT.
RX PubMed=23911548; DOI=10.1016/j.jmb.2013.06.040;
RA Xu J., Hendrix R.W., Duda R.L.;
RT "Chaperone-protein interactions that mediate assembly of the bacteriophage
RT lambda tail to the correct length.";
RL J. Mol. Biol. 426:1004-1018(2014).
RN [5]
RP STRUCTURE BY NMR OF 1-153.
RX PubMed=19251647; DOI=10.1073/pnas.0900044106;
RA Pell L.G., Kanelis V., Donaldson L.W., Howell P.L., Davidson A.R.;
RT "The phage lambda major tail protein structure reveals a common evolution
RT for long-tailed phages and the type VI bacterial secretion system.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:4160-4165(2009).
RN [6]
RP STRUCTURE BY NMR OF 160-246.
RX PubMed=20826161; DOI=10.1016/j.jmb.2010.08.044;
RA Pell L.G., Gasmi-Seabrook G.M., Morais M., Neudecker P., Kanelis V.,
RA Bona D., Donaldson L.W., Edwards A.M., Howell P.L., Davidson A.R.,
RA Maxwell K.L.;
RT "The solution structure of the C-terminal Ig-like domain of the
RT bacteriophage lambda tail tube protein.";
RL J. Mol. Biol. 403:468-479(2010).
CC -!- FUNCTION: Forms the phage's tail tube composed of 32 hexameric disks.
CC When it encounters the appropriate initiation complex gpM and gpL, it
CC assembles in hexameric rings that stack on top of each others.
CC Multimerization ceases when the correct tail length is achieved through
CC a mechanism dependent on tail terminator protein.
CC {ECO:0000269|PubMed:2150582, ECO:0000269|PubMed:23911548}.
CC -!- SUBUNIT: Multimerizes into a structure formed by 32 hexameric rings
CC stacked on phage baseplate gpM and gpL. Does not multimerize in
CC solution without the tail initiation complex. Soluble major tail
CC protein interacts with tail assembly protein GT during tail assembly.
CC {ECO:0000269|PubMed:23911548, ECO:0000269|PubMed:835226}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:835226}. Host
CC cytoplasm {ECO:0000269|PubMed:835226}.
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DR EMBL; J02459; AAA96545.1; -; Genomic_DNA.
DR PIR; D43008; TLBPVL.
DR RefSeq; NP_040592.1; NC_001416.1.
DR PDB; 2K4Q; NMR; -; A=1-153.
DR PDB; 2L04; NMR; -; A=160-246.
DR PDB; 6P3E; EM; 5.40 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R=1-246.
DR PDBsum; 2K4Q; -.
DR PDBsum; 2L04; -.
DR PDBsum; 6P3E; -.
DR BMRB; P03733; -.
DR SMR; P03733; -.
DR IntAct; P03733; 6.
DR GeneID; 2703487; -.
DR KEGG; vg:2703487; -.
DR EvolutionaryTrace; P03733; -.
DR Proteomes; UP000001711; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0098026; C:virus tail, tube; IDA:UniProtKB.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR InterPro; IPR003343; Big_2.
DR InterPro; IPR032494; Phage_TTP_12.
DR Pfam; PF02368; Big_2; 1.
DR Pfam; PF16461; Phage_TTP_12; 1.
DR SMART; SM00635; BID_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host cytoplasm; Reference proteome;
KW Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Viral release from host cell;
KW Viral tail assembly; Viral tail protein; Viral tail tube protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..246
FT /note="Tail tube protein"
FT /id="PRO_0000077671"
FT DOMAIN 161..239
FT /note="BIG2"
FT /evidence="ECO:0000255"
FT DOMAIN 204..246
FT /note="Big-1; incomplete"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00445"
FT STRAND 16..19
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 22..28
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 37..46
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 67..69
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 80..86
FT /evidence="ECO:0007829|PDB:2K4Q"
FT HELIX 93..102
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 106..111
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 131..133
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 136..143
FT /evidence="ECO:0007829|PDB:2K4Q"
FT STRAND 164..167
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 171..174
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 178..185
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 206..210
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 213..217
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 219..225
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 228..230
FT /evidence="ECO:0007829|PDB:2L04"
FT TURN 231..233
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 234..237
FT /evidence="ECO:0007829|PDB:2L04"
FT STRAND 240..244
FT /evidence="ECO:0007829|PDB:2L04"
SQ SEQUENCE 246 AA; 25810 MW; 3E9B2124FF2E80CB CRC64;
MPVPNPTMPV KGAGTTLWVY KGSGDPYANP LSDVDWSRLA KVKDLTPGEL TAESYDDSYL
DDEDADWTAT GQGQKSAGDT SFTLAWMPGE QGQQALLAWF NEGDTRAYKI RFPNGTVDVF
RGWVSSIGKA VTAKEVITRT VKVTNVGRPS MAEDRSTVTA ATGMTVTPAS TSVVKGQSTT
LTVAFQPEGV TDKSFRAVSA DKTKATVSVS GMTITVNGVA AGKVNIPVVS GNGEFAAVAE
ITVTAS
