ID   TUBR_PRIM1              Reviewed;         101 AA.
AC   Q848W2;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=DNA-binding protein TubR {ECO:0000303|PubMed:23010931};
GN   Name=tubR {ECO:0000303|PubMed:23010931}; OrderedLocusNames=BMQ_pBM40059;
OS   Priestia megaterium (strain ATCC 12872 / QMB1551) (Bacillus megaterium).
OG   Plasmid pBM400.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=545693;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12872 / QMB1551; PLASMID=pBM400;
RX   PubMed=14602653; DOI=10.1128/aem.69.11.6888-6898.2003;
RA   Scholle M.D., White C.A., Kunnimalaiyaan M., Vary P.S.;
RT   "Sequencing and characterization of pBM400 from Bacillus megaterium QM
RT   B1551.";
RL   Appl. Environ. Microbiol. 69:6888-6898(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12872 / QMB1551; PLASMID=pBM400;
RX   PubMed=21705586; DOI=10.1128/jb.00449-11;
RA   Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K.,
RA   Koenig S.S., Creasy H.H., Rosovitz M.J., Riley D.R., Daugherty S.,
RA   Martin M., Elbourne L.D., Paulsen I., Biedendieck R., Braun C.,
RA   Grayburn S., Dhingra S., Lukyanchuk V., Ball B., Ul-Qamar R., Seibel J.,
RA   Bremer E., Jahn D., Ravel J., Vary P.S.;
RT   "Genome sequences of the biotechnologically important Bacillus megaterium
RT   strains QM B1551 and DSM319.";
RL   J. Bacteriol. 193:4199-4213(2011).
RN   [3] {ECO:0007744|PDB:4ASN}
RP   X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS), FUNCTION, SUBUNIT, AND DNA-BINDING.
RC   STRAIN=ATCC 12872 / QMB1551; PLASMID=pBM400;
RX   PubMed=23010931; DOI=10.1073/pnas.1210899109;
RA   Aylett C.H., Lowe J.;
RT   "Superstructure of the centromeric complex of TubZRC plasmid partitioning
RT   systems.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:16522-16527(2012).
CC   -!- FUNCTION: A DNA-binding protein that is part of the type III plasmid
CC       partition system used to ensure correct segregation of the pBM400
CC       plasmid. Binds the plasmid origin of replication, probably
CC       cooperatively, forming a ring or short helix with external DNA
CC       (PubMed:23010931). Its effect on RNA expression has not been shown
CC       (Probable). {ECO:0000269|PubMed:23010931, ECO:0000305}.
CC   -!- SUBUNIT: Homodimer. Dimers bind to DNA, forming a protein-bound
CC       filament which may form a helix around the TubZ filament.
CC       {ECO:0000269|PubMed:23010931}.
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DR   EMBL; CP001987; AAO52804.1; -; Genomic_DNA.
DR   RefSeq; WP_011106199.1; NC_004604.2.
DR   PDB; 4ASN; X-ray; 3.50 A; A/B/C=1-101.
DR   PDBsum; 4ASN; -.
DR   AlphaFoldDB; Q848W2; -.
DR   SMR; Q848W2; -.
DR   EnsemblBacteria; AAO52804; AAO52804; BMQ_pBM40059.
DR   KEGG; bmq:BMQ_pBM40059; -.
DR   HOGENOM; CLU_2380079_0_0_9; -.
DR   OrthoDB; 2176518at2; -.
DR   Proteomes; UP000000935; Plasmid pBM400.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030541; P:plasmid partitioning; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Plasmid; Plasmid partition; Reference proteome.
FT   CHAIN           1..101
FT                   /note="DNA-binding protein TubR"
FT                   /id="PRO_0000448566"
FT   HELIX           3..10
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   HELIX           14..25
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   HELIX           31..33
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   HELIX           37..41
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   HELIX           49..60
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   STRAND          64..67
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   STRAND          69..72
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   STRAND          74..77
FT                   /evidence="ECO:0007829|PDB:4ASN"
FT   HELIX           79..88
FT                   /evidence="ECO:0007829|PDB:4ASN"
SQ   SEQUENCE   101 AA;  11593 MW;  FB2A79588FE55417 CRC64;
     MSDYFEEVMR KLTIEDVSIL GWLFQNEANA VFKAIKKSSI ADELEYSTAN FRKTLNKLEA
     IHFIGTVTGG KEHKLYLTEY GQQAVQQAIH HGEENEEVEE I