ID   TUFT1_BOVIN             Reviewed;         390 AA.
AC   P27628; O97683;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2002, sequence version 2.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=Tuftelin {ECO:0000303|PubMed:1874744};
DE   AltName: Full=Enamelin;
GN   Name=TUFT1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   STRAIN=Holstein; TISSUE=Ameloblast;
RX   PubMed=1874744; DOI=10.1016/s0021-9258(18)98510-8;
RA   Deutsch D., Palmon A., Fisher L.W., Kolodny N., Termine J.D., Young M.F.;
RT   "Sequencing of bovine enamelin ('tuftelin') a novel acidic enamel
RT   protein.";
RL   J. Biol. Chem. 266:16021-16028(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RC   TISSUE=Enamel organ;
RX   PubMed=9296268; DOI=10.1016/s0003-9969(97)00039-3;
RA   Bashir M.M., Abrams W.R., Rosenbloom J.;
RT   "Molecular cloning and characterization of the bovine tuftelin gene.";
RL   Arch. Oral Biol. 42:489-496(1997).
RN   [3]
RP   SEQUENCE REVISION.
RA   Bashir M.M., Abrams W.R., Rosenbloom J.;
RL   Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the mineralization and structural organization of
CC       enamel. {ECO:0000303|PubMed:1874744}.
CC   -!- SUBUNIT: Interacts with TFIP11. {ECO:0000250|UniProtKB:O08970}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000303|PubMed:1874744}.
CC       Note=Secreted at a very early stage of enamel formation, and tightly
CC       bound to the surface of the growing crystallites.
CC       {ECO:0000303|PubMed:1874744}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1;
CC         IsoId=P27628-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P27628-2; Sequence=VSP_006682;
CC       Name=3;
CC         IsoId=P27628-3; Sequence=VSP_006683;
CC       Name=4;
CC         IsoId=P27628-4; Sequence=VSP_006684;
CC   -!- TISSUE SPECIFICITY: Present in the extracellular enamel and is mainly
CC       associated with the crystal component. {ECO:0000269|PubMed:1874744}.
CC   -!- SIMILARITY: Belongs to the tuftelin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA30797.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M64924; AAA30797.1; ALT_FRAME; mRNA.
DR   EMBL; AF105228; AAC84147.1; -; mRNA.
DR   PIR; A40809; A40809.
DR   RefSeq; NP_776904.1; NM_174479.2. [P27628-1]
DR   AlphaFoldDB; P27628; -.
DR   SMR; P27628; -.
DR   STRING; 9913.ENSBTAP00000043710; -.
DR   PaxDb; P27628; -.
DR   GeneID; 282104; -.
DR   KEGG; bta:282104; -.
DR   CTD; 7286; -.
DR   eggNOG; ENOG502QW76; Eukaryota.
DR   InParanoid; P27628; -.
DR   OrthoDB; 1003198at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042802; F:identical protein binding; IPI:BHF-UCL.
DR   GO; GO:0031214; P:biomineral tissue development; IEA:UniProtKB-KW.
DR   InterPro; IPR024846; Tuftelin.
DR   PANTHER; PTHR23171:SF4; PTHR23171:SF4; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Biomineralization; Coiled coil; Phosphoprotein;
KW   Reference proteome; Secreted.
FT   CHAIN           1..390
FT                   /note="Tuftelin"
FT                   /id="PRO_0000183185"
FT   COILED          88..126
FT                   /evidence="ECO:0000255"
FT   COILED          162..351
FT                   /evidence="ECO:0000255"
FT   MOD_RES         171
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         21..45
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_006682"
FT   VAR_SEQ         109..138
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_006683"
FT   VAR_SEQ         139..160
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_006684"
FT   CONFLICT        242
FT                   /note="E -> D (in Ref. 2; AAA30797)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   390 AA;  44343 MW;  E276261E098E3444 CRC64;
     MNGTRNWCTL VDVHPEGQTA GSVDVLRLTL QSELTGDELE RIAQKAGRKT YAMVSSHSTS
     HSLASELVES NDGHEEIIKV YLKGRSGDKM IHEKNINQLK SEVQYIQEAR NCLQKLREDI
     SSKLDRDPGD SVHKQEIQVV LEKQNGLSEG PLTTYSSPPE VDTHINEDVE SLRKTVQDLL
     VKLQEAEQQH QSDCSAFKVT LSQYQREAKQ SQVALQRAED RAEQKEAEVG ELQRRLQGME
     TEYQAILAKV REGETALEEL RSKNVDCQAE QEKAANLEKE VAGLREKIHH LDDMLKSQQR
     KVRQMIEQLQ NSKAVIQSKD TTIQELKEKI AYLEAENLEM HDRMEHLIEK QISHGNFSTQ
     NRAKTENLGS IRISKPPSPK PMPLIRVVET