TUFT1_MOUSE
ID TUFT1_MOUSE Reviewed; 390 AA.
AC O08970;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Tuftelin;
GN Name=Tuft1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC STRAIN=Swiss Webster; TISSUE=Tooth;
RX PubMed=9839784; DOI=10.1177/00220345980770120401;
RA MacDougall M., Simmons D., Dodds A., Knight C., Luan X., Zeichner-David M.,
RA Zhang C., Ryu O.H., Qian Q., Simmer J.P., Hu C.-C.;
RT "Cloning, characterization, and tissue expression pattern of mouse tuftelin
RT cDNA.";
RL J. Dent. Res. 77:1970-1978(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
RX PubMed=11733143; DOI=10.1016/s0378-1119(01)00749-1;
RA Mao Z., Shay B., Hekmati M., Fermon E., Taylor A., Dafni L.,
RA Heikinheimo K., Lustmann J., Fisher L.W., Young M.F., Deutsch D.;
RT "The human tuftelin gene: cloning and characterization.";
RL Gene 279:181-196(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 7-324 (ISOFORM 1), AND CHARACTERIZATION.
RC STRAIN=Swiss Webster;
RX PubMed=9074935;
RA Zeichner-David M., Vo H., Tan H., Diekwisch T., Berman B., Thiemann F.,
RA Alcocer M.D., Hsu P., Wang T., Eyna J., Caton J., Slavkin H.C.,
RA MacDougall M.;
RT "Timing of the expression of enamel gene products during mouse tooth
RT development.";
RL Int. J. Dev. Biol. 41:27-38(1997).
RN [5]
RP INTERACTION WITH TFIP11.
RC STRAIN=Swiss Webster; TISSUE=Tooth;
RX PubMed=10806191; DOI=10.1074/jbc.m000118200;
RA Paine C.T., Paine M.L., Luo W., Okamoto C.T., Lyngstadaas S.P., Snead M.L.;
RT "A tuftelin-interacting protein (TIP39) localizes to the apical secretory
RT pole of mouse ameloblasts.";
RL J. Biol. Chem. 275:22284-22292(2000).
CC -!- FUNCTION: Involved in the mineralization and structural organization of
CC enamel. {ECO:0000250|UniProtKB:P27628}.
CC -!- SUBUNIT: Interacts with TFIP11. May form oligomers.
CC {ECO:0000269|PubMed:10806191}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P27628}.
CC Note=Secreted at a very early stage of enamel formation, concentrated
CC at the dentin-enamel junction and tightly bound to the surface of the
CC growing crystallites. {ECO:0000250|UniProtKB:P27628}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O08970-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O08970-2; Sequence=VSP_006687;
CC Name=3;
CC IsoId=O08970-3; Sequence=VSP_006687, VSP_006688;
CC -!- TISSUE SPECIFICITY: Ameloblasts, and also non-odontogenic tissues
CC including kidney, lung, liver and testis. {ECO:0000269|PubMed:9839784}.
CC -!- DEVELOPMENTAL STAGE: Expressed in tooth from 13 dpc, the bud stage.
CC Continues to be expressed even when thick enamel is formed.
CC -!- SIMILARITY: Belongs to the tuftelin family. {ECO:0000305}.
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DR EMBL; AF047704; AAC04577.1; -; mRNA.
DR EMBL; BC019213; AAH19213.1; -; mRNA.
DR EMBL; AF002860; AAB60891.1; -; mRNA.
DR CCDS; CCDS17595.1; -. [O08970-1]
DR CCDS; CCDS79975.1; -. [O08970-2]
DR RefSeq; NP_035786.1; NM_011656.3. [O08970-1]
DR AlphaFoldDB; O08970; -.
DR SMR; O08970; -.
DR IntAct; O08970; 2.
DR STRING; 10090.ENSMUSP00000006123; -.
DR PhosphoSitePlus; O08970; -.
DR PaxDb; O08970; -.
DR PeptideAtlas; O08970; -.
DR PRIDE; O08970; -.
DR ProteomicsDB; 300060; -. [O08970-1]
DR ProteomicsDB; 300061; -. [O08970-2]
DR ProteomicsDB; 300062; -. [O08970-3]
DR Antibodypedia; 34066; 110 antibodies from 21 providers.
DR Ensembl; ENSMUST00000006123; ENSMUSP00000006123; ENSMUSG00000005968. [O08970-1]
DR Ensembl; ENSMUST00000196733; ENSMUSP00000143278; ENSMUSG00000005968. [O08970-2]
DR GeneID; 22156; -.
DR KEGG; mmu:22156; -.
DR UCSC; uc008qgt.2; mouse. [O08970-1]
DR CTD; 7286; -.
DR MGI; MGI:109572; Tuft1.
DR VEuPathDB; HostDB:ENSMUSG00000005968; -.
DR eggNOG; ENOG502QW76; Eukaryota.
DR GeneTree; ENSGT00950000183065; -.
DR InParanoid; O08970; -.
DR OMA; VPENDFR; -.
DR PhylomeDB; O08970; -.
DR TreeFam; TF331627; -.
DR BioGRID-ORCS; 22156; 3 hits in 70 CRISPR screens.
DR PRO; PR:O08970; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; O08970; protein.
DR Bgee; ENSMUSG00000005968; Expressed in tail skin and 154 other tissues.
DR ExpressionAtlas; O08970; baseline and differential.
DR Genevisible; O08970; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0031214; P:biomineral tissue development; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR InterPro; IPR024846; Tuftelin.
DR PANTHER; PTHR23171:SF4; PTHR23171:SF4; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Biomineralization; Coiled coil; Reference proteome;
KW Secreted.
FT CHAIN 1..390
FT /note="Tuftelin"
FT /id="PRO_0000183187"
FT REGION 356..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 88..126
FT /evidence="ECO:0000255"
FT COILED 163..352
FT /evidence="ECO:0000255"
FT VAR_SEQ 21..45
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:11733143,
FT ECO:0000303|PubMed:9839784"
FT /id="VSP_006687"
FT VAR_SEQ 46..80
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11733143"
FT /id="VSP_006688"
SQ SEQUENCE 390 AA; 44576 MW; 968FA7BBE95DFEC1 CRC64;
MNGTRNWCTL VDVHPESQTA GSVDILRLTL QSELTGDELE HIAQKAGRKT YAMMPGHSSG
HSLASELVES HDGHEEIIKV YLKGRSGDKM THEKNIDQLK SEVQYIQEAR NCLQKLREDI
SSKLDRSPGD PLRQQEIQVV LEKPNGFSQS PMTLYSSPPE VDPSMSEDVE SLKKTVQELL
VKLREAERRH QSDRVAFEVT LSRYQREAEQ SNVALQREED RVEQKAAEIE ELQRRLLGME
AEHQALLVKV REGEMALEEL RIKNADCQTE REKSAALEKE VAGFREKIHH LDDMLKSQQR
KVRQMIEQLQ NSKAVIQSKD ATIQELKEKI AYLEAENLEM HDRMEHLIEK QVSHGNFSTQ
ARAKTENLGS VRISKPPSPK PMPLIRVVET
