TULP2_MOUSE
ID TULP2_MOUSE Reviewed; 562 AA.
AC P46686; Q3TTV1; Q5FW93; Q8C5R7; Q8C5R9; Q9R1I8;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Tubby-related protein 2;
DE AltName: Full=Protein P4-6;
DE AltName: Full=Tubby-like protein 2;
GN Name=Tulp2; Synonyms=Pdet;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=BALB/cJ;
RA Ikeda S., Sorokina I., Naggert J.K., North M.A., Nishina P.M.;
RT "Apoptotic photoreceptor cell death in tubby mice and the localization of
RT tubby gene family members in the retina.";
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 5).
RC STRAIN=C57BL/6J; TISSUE=Head, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 245-562 (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=7509194; DOI=10.1016/0167-4781(94)90036-1;
RA Vambutas V., Wolgemuth D.J.;
RT "Identification and characterization of the developmentally regulated
RT pattern of expression in the testis of a mouse gene exhibiting similarity
RT to the family of phosphodiesterases.";
RL Biochim. Biophys. Acta 1217:203-206(1994).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=19695251; DOI=10.1016/j.febslet.2009.08.015;
RA Caberoy N.B., Li W.;
RT "Unconventional secretion of tubby and tubby-like protein 1.";
RL FEBS Lett. 583:3057-3062(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152; SER-153; SER-155;
RP THR-211 AND SER-213, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19695251}. Secreted
CC {ECO:0000269|PubMed:19695251}. Note=Does not have a cleavable signal
CC peptide and is secreted by a non-conventional pathway.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=P46686-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P46686-2; Sequence=VSP_022199;
CC Name=3;
CC IsoId=P46686-3; Sequence=VSP_022197, VSP_022199;
CC Name=4;
CC IsoId=P46686-4; Sequence=VSP_022197, VSP_022198, VSP_022199;
CC Name=5;
CC IsoId=P46686-5; Sequence=VSP_022197, VSP_022200;
CC -!- TISSUE SPECIFICITY: Expressed in retina and testis.
CC -!- SIMILARITY: Belongs to the TUB family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be a phosphodiesterase on the basis
CC of spurious sequence similarities. {ECO:0000305|PubMed:7509194}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD38452.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC36686.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA49481.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF105712; AAD38452.1; ALT_INIT; mRNA.
DR EMBL; AK077200; BAC36678.1; -; mRNA.
DR EMBL; AK077211; BAC36686.1; ALT_INIT; mRNA.
DR EMBL; AK161165; BAE36223.1; -; mRNA.
DR EMBL; BC089545; AAH89545.1; -; mRNA.
DR EMBL; X69827; CAA49481.1; ALT_INIT; mRNA.
DR CCDS; CCDS39953.1; -. [P46686-1]
DR CCDS; CCDS39954.1; -. [P46686-2]
DR CCDS; CCDS71960.1; -. [P46686-3]
DR CCDS; CCDS80720.1; -. [P46686-4]
DR RefSeq; NP_001039020.1; NM_001045555.2. [P46686-1]
DR RefSeq; NP_001277927.1; NM_001290998.2. [P46686-4]
DR RefSeq; NP_001277928.1; NM_001290999.2. [P46686-3]
DR RefSeq; NP_032833.2; NM_008807.3. [P46686-2]
DR RefSeq; XP_006541088.1; XM_006541025.3. [P46686-5]
DR AlphaFoldDB; P46686; -.
DR SMR; P46686; -.
DR STRING; 10090.ENSMUSP00000103391; -.
DR iPTMnet; P46686; -.
DR PhosphoSitePlus; P46686; -.
DR PaxDb; P46686; -.
DR PRIDE; P46686; -.
DR ProteomicsDB; 298062; -. [P46686-1]
DR ProteomicsDB; 298063; -. [P46686-2]
DR ProteomicsDB; 298064; -. [P46686-3]
DR ProteomicsDB; 298065; -. [P46686-4]
DR ProteomicsDB; 298066; -. [P46686-5]
DR Antibodypedia; 31829; 188 antibodies from 23 providers.
DR DNASU; 56734; -.
DR Ensembl; ENSMUST00000024233; ENSMUSP00000024233; ENSMUSG00000023467. [P46686-2]
DR Ensembl; ENSMUST00000085331; ENSMUSP00000082438; ENSMUSG00000023467. [P46686-4]
DR Ensembl; ENSMUST00000107759; ENSMUSP00000103388; ENSMUSG00000023467. [P46686-3]
DR Ensembl; ENSMUST00000107762; ENSMUSP00000103391; ENSMUSG00000023467. [P46686-1]
DR Ensembl; ENSMUST00000210868; ENSMUSP00000147710; ENSMUSG00000023467. [P46686-5]
DR GeneID; 56734; -.
DR KEGG; mmu:56734; -.
DR UCSC; uc009gvk.2; mouse. [P46686-3]
DR UCSC; uc009gvp.2; mouse. [P46686-2]
DR UCSC; uc009gvq.2; mouse. [P46686-1]
DR UCSC; uc009gvr.2; mouse. [P46686-4]
DR CTD; 7288; -.
DR MGI; MGI:1861600; Tulp2.
DR VEuPathDB; HostDB:ENSMUSG00000023467; -.
DR eggNOG; KOG2502; Eukaryota.
DR GeneTree; ENSGT00940000161908; -.
DR HOGENOM; CLU_028236_0_0_1; -.
DR InParanoid; P46686; -.
DR OMA; VCYEPNI; -.
DR PhylomeDB; P46686; -.
DR TreeFam; TF314076; -.
DR BioGRID-ORCS; 56734; 4 hits in 56 CRISPR screens.
DR ChiTaRS; Tulp2; mouse.
DR PRO; PR:P46686; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P46686; protein.
DR Bgee; ENSMUSG00000023467; Expressed in seminiferous tubule of testis and 59 other tissues.
DR ExpressionAtlas; P46686; baseline and differential.
DR Genevisible; P46686; MM.
DR GO; GO:0005929; C:cilium; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008081; F:phosphoric diester hydrolase activity; ISS:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0061512; P:protein localization to cilium; IBA:GO_Central.
DR Gene3D; 3.20.90.10; -; 1.
DR InterPro; IPR025659; Tubby-like_C.
DR InterPro; IPR000007; Tubby_C.
DR InterPro; IPR018066; Tubby_C_CS.
DR InterPro; IPR005398; Tubby_N.
DR Pfam; PF01167; Tub; 1.
DR Pfam; PF16322; Tub_N; 1.
DR PRINTS; PR01573; SUPERTUBBY.
DR PRINTS; PR01574; TUBBYPROTEIN.
DR SUPFAM; SSF54518; SSF54518; 1.
DR PROSITE; PS01200; TUB_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome;
KW Secreted.
FT CHAIN 1..562
FT /note="Tubby-related protein 2"
FT /id="PRO_0000186469"
FT REGION 1..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 179..294
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..56
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..79
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..215
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 249..263
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 152
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 153
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 155
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 211
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 213
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..112
FT /note="Missing (in isoform 3, isoform 4 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_022197"
FT VAR_SEQ 121..132
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022198"
FT VAR_SEQ 561..562
FT /note="FF -> CE (in isoform 2, isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072, ECO:0000303|PubMed:7509194,
FT ECO:0000303|Ref.1"
FT /id="VSP_022199"
FT VAR_SEQ 561..562
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_022200"
FT CONFLICT 1
FT /note="M -> R (in Ref. 1; AAD38452)"
FT /evidence="ECO:0000305"
FT CONFLICT 314
FT /note="L -> V (in Ref. 4; CAA49481)"
FT /evidence="ECO:0000305"
FT CONFLICT 324
FT /note="C -> W (in Ref. 1; AAD38452 and 4; CAA49481)"
FT /evidence="ECO:0000305"
FT CONFLICT 347
FT /note="A -> G (in Ref. 1; AAD38452 and 4; CAA49481)"
FT /evidence="ECO:0000305"
FT CONFLICT 504
FT /note="T -> S (in Ref. 4; CAA49481)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 562 AA; 62724 MW; 0FD479941D6F7144 CRC64;
MDREGPRGPR SGASQENEQW KKETLEDEFS GVRLQKLEQQ RQLFEKKQRR KRQEPLMVQA
NPDATLRHRR PRRGEERFQS DSSWGLGVGS PFLQENVPQA HLPSGAHSAL VTMSYVADGS
GERAPLLSPR GAVYTRGNGP AVRHHLCWLP DSSDSDVEEV TMEDIPVISR PPQTNLANLR
RGWLASPGPG ISQEEKEEEV GSTDARVEDK TPSPDPDPDP TVNSDGDHGD LAPCKVEENT
AQKNTETASG IGDEDREKGE VTESTETNYA PVASKVLQGD DGDASNHNAW NMTCPQPRIP
GPRLGEDMEA YVLLPAPRDH MVQCRIVRNK HGMDKGMFPS YYLYLEAEDG VAHFLLAGRK
RKRSKTSNYL ISLDPKDMSR NGSNFVGKVR SNVLGTKFTI FDNGVNPERS YWVPDSARIR
EELGVVCYET NVLGFRGPRK MTVILPGMDS RKQRMKVQPQ NDQDSILSRV QKGAGHGLLL
LQNKAPSWSD ESGAYVLNFH GRVTRASVKN FQIVHPDEPD HLVLQFGRVA PNIFTMDFRY
PLCPLQAFAI CLSSFDGKLA FF
