TULP4_MOUSE
ID TULP4_MOUSE Reviewed; 1547 AA.
AC Q9JIL5; B2RQP8; Q8CA75; Q922C2;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Tubby-related protein 4;
DE AltName: Full=Tubby superfamily protein;
DE AltName: Full=Tubby-like protein 4;
GN Name=Tulp4; Synonyms=Tusp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RX PubMed=11595174; DOI=10.1016/s0378-1119(01)00582-0;
RA Li Q.-Z., Wang C.-Y., Shi J.-D., Ruan Q.-G., Eckenrode S.,
RA Davoodi-Semiromi A., Kukar T., Gu Y., Lian W., Wu D., She J.-X.;
RT "Molecular cloning and characterization of the mouse and human TUSP gene, a
RT novel member of the tubby superfamily.";
RL Gene 273:275-284(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1122-1547.
RC STRAIN=C57BL/6J; TISSUE=Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-949 AND ARG-954, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: May be a substrate-recognition component of a SCF-like ECS
CC (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which
CC mediates the ubiquitination and subsequent proteasomal degradation of
CC target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin ligase
CC complexes. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TUB family. {ECO:0000305}.
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DR EMBL; AF219945; AAF87974.1; -; mRNA.
DR EMBL; AK039439; BAC30348.1; -; mRNA.
DR EMBL; BC008557; AAH08557.1; -; mRNA.
DR EMBL; BC138024; AAI38025.1; -; mRNA.
DR EMBL; BC138025; AAI38026.1; -; mRNA.
DR CCDS; CCDS28370.1; -.
DR RefSeq; NP_473381.1; NM_054040.3.
DR RefSeq; XP_006523314.1; XM_006523251.3.
DR AlphaFoldDB; Q9JIL5; -.
DR SMR; Q9JIL5; -.
DR STRING; 10090.ENSMUSP00000049248; -.
DR iPTMnet; Q9JIL5; -.
DR PhosphoSitePlus; Q9JIL5; -.
DR EPD; Q9JIL5; -.
DR MaxQB; Q9JIL5; -.
DR PaxDb; Q9JIL5; -.
DR PRIDE; Q9JIL5; -.
DR ProteomicsDB; 298029; -.
DR Antibodypedia; 1688; 58 antibodies from 18 providers.
DR DNASU; 68842; -.
DR Ensembl; ENSMUST00000039655; ENSMUSP00000049248; ENSMUSG00000034377.
DR GeneID; 68842; -.
DR KEGG; mmu:68842; -.
DR UCSC; uc008agf.2; mouse.
DR CTD; 56995; -.
DR MGI; MGI:1916092; Tulp4.
DR VEuPathDB; HostDB:ENSMUSG00000034377; -.
DR eggNOG; KOG2503; Eukaryota.
DR GeneTree; ENSGT00940000155913; -.
DR HOGENOM; CLU_002083_1_0_1; -.
DR InParanoid; Q9JIL5; -.
DR OMA; DARVKCM; -.
DR PhylomeDB; Q9JIL5; -.
DR TreeFam; TF314076; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 68842; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Tulp4; mouse.
DR PRO; PR:Q9JIL5; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9JIL5; protein.
DR Bgee; ENSMUSG00000034377; Expressed in endothelial cell of lymphatic vessel and 270 other tissues.
DR ExpressionAtlas; Q9JIL5; baseline and differential.
DR Genevisible; Q9JIL5; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 3.20.90.10; -; 1.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR025659; Tubby-like_C.
DR InterPro; IPR000007; Tubby_C.
DR InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF01167; Tub; 1.
DR SMART; SM00969; SOCS_box; 1.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF49842; SSF49842; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF54518; SSF54518; 1.
DR PROSITE; PS50225; SOCS; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methylation; Phosphoprotein; Reference proteome; Repeat;
KW Ubl conjugation pathway; WD repeat.
FT CHAIN 1..1547
FT /note="Tubby-related protein 4"
FT /id="PRO_0000186473"
FT REPEAT 80..119
FT /note="WD 1"
FT REPEAT 123..162
FT /note="WD 2"
FT REPEAT 165..204
FT /note="WD 3"
FT DOMAIN 364..414
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
FT REGION 530..580
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1374..1414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1436..1547
FT /note="TUB"
FT COMPBIAS 1380..1409
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 577
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NRJ4"
FT MOD_RES 949
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 954
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 1347
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NRJ4"
FT MOD_RES 1378
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NRJ4"
FT CONFLICT 1420
FT /note="G -> R (in Ref. 3; BAC30348)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1547 AA; 169638 MW; 8B7CA7A250884423 CRC64;
MYAAVEHGPV LCSDSNILCL SWKGRVPKSE KEKPVCRRRY YEEGWLATGN GRGVVGVTFT
SSHCRRDRST PQRINFNLRG HNSEVVLVRW NEPYQKLATC DADGGIFVWI QYEGRWSVEL
VNDRGAQVSD FTWSHDGTQA LISYRDGFVL VGSVSGQRHW SSEINLESQI TCGIWTPDDQ
QVLFGTADGQ VIVMDCHGRM LAHVLLHESD GILSMSWNYP IFLVEDSSES DTDSDDYSPP
QDGPAAYPIP VQNTKPLLTV SFTSGDISLM NNYDDLSPTV IRSGLKEVVA QWCTQGDLLA
VAGMEQQAQL SELPNGPLLK SAMVKFYNVR GEHIFTLDTL VQRPIISICW GHRDSRLLMA
SGPALYVVRV EHRVSSLQLL CQQAIASTLR EDKDVNKLTL PPRLCSYLST AFIPTIKPPI
PDPNNMRDFV SYPSAGNERL HCTMKRTEDD PEVGGPCYTL YLEYLGGLVP ILKGRRISKL
RPEFVIMDPR TDSKSDEIYG NSLISTVIDS CNCSDSSDIE LSDDWAAKKS PKISRSSKSP
KLPRISIEAR KSPKLPRAAQ EISRSPRLPM RKPSMGSPSL TRREFPFEDI TQHNYLAQVT
SNIWGTKFKI VGLAAFLPTN LGAVIYKTSL LHLQPRQMTI YLPEVRKISM DYINLPVFNP
NVFSEDEDDL PVTGASGVPE NNPPCTVNIP IAPIHSSAQA LSPTQSIGLV QSLLANQNVQ
LDVLTNQTTA VGSAEHAGDA ATQYPVSSRY SNPGQVIFGG VEMGRIIQNP PQLPLPPPPP
PPPQAPMQLS AVDHGDRDHE HLQKSAKALR PVPQLAAEGD AVVFSAPQEV QVAKMNPPPP
YPGTIPAAPT TAAPPPPLPP PQPPVDVCLK KGDFSLYPTA AHYQPPLGYE RITTFDSSGN
VEEVCRPRTR MLCSQNTYTL PGPGSSATLR LTATEKKVPQ PCTSATLNRL TVPRYSIPTG
DPPPYPEIAS QLAQGRSAAQ RLDNSLIHAT LRRNNREVAL KMAQLADSSR APLQPLAKPK
GGAAGAVAQL PARPPPALYT CSQCSGAGPS SQSGAALAHA ISTSPLASQS SYNLLSPPDT
SRDRTDYVNS AFTEDEALSQ HCQLEKPLRH PPLPEAAVTM KRPPPYQWDP MLGEDVWVPQ
ERTAQPTVPN PLKLSPLMLG QGQHLDVARV PFVPPKSPSS PTATFPTGYG MGMPYPGSYN
NPSLPGVQAP CSPKDALSQA QFAQQESAVV LQPAYPPSLS YCTLPPTYPG SSTCSSVQLP
PIALHPWNSY STCPPMQNTQ GTLPPKPHLV VEKPLVSPPP AELQSHMGTE VMVETADNFQ
EVLSLTESPV PQRTEKFGKK NRKRLDSRAE EGSVQAITEG KVKKDARTLS DFNSLISSPR
LGREKKKVKS QKDQLKSKKL NKTNEFQDSS ESEPELFISG DELMNQSQGS KKGWKSKRSL
RTASELEEFK CRKASEKEDG RLGSQGFVYV MANKQPLWNE ATQVYQLDFG GRVTQESAKN
FQIELEGRQV MQFGRIDGNA YILDFQYPFS AVQAFAVALA NVTQRLK
