TUP1_CANAL
ID TUP1_CANAL Reviewed; 512 AA.
AC P0CY34; A0A1D8PC37; P56093; Q5AB65;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Transcriptional repressor TUP1;
GN Name=TUP1; Synonyms=CHP1; OrderedLocusNames=CAALFM_C100060WA;
GN ORFNames=CaO19.13528, CaO19.6109;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION.
RX PubMed=9204892; DOI=10.1126/science.277.5322.105;
RA Braun B.R., Johnson A.D.;
RT "Control of filament formation in Candida albicans by the transcriptional
RT repressor TUP1.";
RL Science 277:105-109(1997).
RN [5]
RP FUNCTION.
RX PubMed=9809417; DOI=10.1111/j.1574-6968.1998.tb13223.x;
RA Hoover C.I., Jantapour M.J., Newport G., Agabian N., Fisher S.J.;
RT "Cloning and regulated expression of the Candida albicans phospholipase B
RT (PLB1) gene.";
RL FEMS Microbiol. Lett. 167:163-169(1998).
RN [6]
RP FUNCTION.
RX PubMed=10790384; DOI=10.1093/genetics/155.1.57;
RA Braun B.R., Johnson A.D.;
RT "TUP1, CPH1 and EFG1 make independent contributions to filamentation in
RT Candida albicans.";
RL Genetics 155:57-67(2000).
RN [7]
RP FUNCTION.
RX PubMed=10978273; DOI=10.1093/genetics/156.1.31;
RA Braun B.R., Head W.S., Wang M.X., Johnson A.D.;
RT "Identification and characterization of TUP1-regulated genes in Candida
RT albicans.";
RL Genetics 156:31-44(2000).
RN [8]
RP FUNCTION.
RX PubMed=10816502; DOI=10.1128/iai.68.6.3485-3490.2000;
RA Phan Q.T., Belanger P.H., Filler S.G.;
RT "Role of hyphal formation in interactions of Candida albicans with
RT endothelial cells.";
RL Infect. Immun. 68:3485-3490(2000).
RN [9]
RP FUNCTION.
RX PubMed=11532938; DOI=10.1093/emboj/20.17.4742;
RA Murad A.M., Leng P., Straffon M., Wishart J., Macaskill S., MacCallum D.,
RA Schnell N., Talibi D., Marechal D., Tekaia F., d'Enfert C., Gaillardin C.,
RA Odds F.C., Brown A.J.;
RT "NRG1 represses yeast-hypha morphogenesis and hypha-specific gene
RT expression in Candida albicans.";
RL EMBO J. 20:4742-4752(2001).
RN [10]
RP FUNCTION.
RX PubMed=11259598; DOI=10.1128/mcb.21.7.2496-2505.2001;
RA Kadosh D., Johnson A.D.;
RT "Rfg1, a protein related to the Saccharomyces cerevisiae hypoxic regulator
RT Rox1, controls filamentous growth and virulence in Candida albicans.";
RL Mol. Cell. Biol. 21:2496-2505(2001).
RN [11]
RP FUNCTION.
RX PubMed=11737641; DOI=10.1046/j.1365-2958.2001.02713.x;
RA Murad A.M., d'Enfert C., Gaillardin C., Tournu H., Tekaia F., Talibi D.,
RA Marechal D., Marchais V., Cottin J., Brown A.J.;
RT "Transcript profiling in Candida albicans reveals new cellular functions
RT for the transcriptional repressors CaTup1, CaMig1 and CaNrg1.";
RL Mol. Microbiol. 42:981-993(2001).
RN [12]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12455984; DOI=10.1128/ec.1.3.353-365.2002;
RA Zhao R., Lockhart S.R., Daniels K., Soll D.R.;
RT "Roles of TUP1 in switching, phase maintenance, and phase-specific gene
RT expression in Candida albicans.";
RL Eukaryot. Cell 1:353-365(2002).
RN [13]
RP INDUCTION.
RX PubMed=15673737; DOI=10.1128/aac.49.2.584-589.2005;
RA Cao Y.Y., Cao Y.B., Xu Z., Ying K., Li Y., Xie Y., Zhu Z.Y., Chen W.S.,
RA Jiang Y.Y.;
RT "cDNA microarray analysis of differential gene expression in Candida
RT albicans biofilm exposed to farnesol.";
RL Antimicrob. Agents Chemother. 49:584-589(2005).
RN [14]
RP FUNCTION.
RX PubMed=15814840; DOI=10.1091/mbc.e05-01-0073;
RA Kadosh D., Johnson A.D.;
RT "Induction of the Candida albicans filamentous growth program by relief of
RT transcriptional repression: a genome-wide analysis.";
RL Mol. Biol. Cell 16:2903-2912(2005).
RN [15]
RP FUNCTION.
RX PubMed=16313617; DOI=10.1111/j.1365-2958.2005.04898.x;
RA Park Y.N., Morschhauser J.;
RT "Candida albicans MTLalpha tup1Delta mutants can reversibly switch to
RT mating-competent, filamentous growth forms.";
RL Mol. Microbiol. 58:1288-1302(2005).
RN [16]
RP FUNCTION.
RX PubMed=18424510; DOI=10.1128/ec.00357-07;
RA Kebaara B.W., Langford M.L., Navarathna D.H., Dumitru R., Nickerson K.W.,
RA Atkin A.L.;
RT "Candida albicans Tup1 is involved in farnesol-mediated inhibition of
RT filamentous-growth induction.";
RL Eukaryot. Cell 7:980-987(2008).
CC -!- FUNCTION: Represses transcription by RNA polymerase II. Represses genes
CC responsible for initiating filamentous growth such as HWP1, RBT1, RBT2,
CC RBT4, RBT5, RBT7 and WAP1; and this repression is lifted under inducing
CC environmental conditions. Represses also genes which participate in
CC pathogenesis. Crucial component of the response to farnesol. Plays an
CC important role in the regulation of white-opaque switching.
CC {ECO:0000269|PubMed:10790384, ECO:0000269|PubMed:10816502,
CC ECO:0000269|PubMed:10978273, ECO:0000269|PubMed:11259598,
CC ECO:0000269|PubMed:11532938, ECO:0000269|PubMed:11737641,
CC ECO:0000269|PubMed:12455984, ECO:0000269|PubMed:15814840,
CC ECO:0000269|PubMed:16313617, ECO:0000269|PubMed:18424510,
CC ECO:0000269|PubMed:9204892, ECO:0000269|PubMed:9809417}.
CC -!- INDUCTION: Expression is more than fourfold higher in white-phase cells
CC than in opaque-phase cells. Up-regulated by farnesol in biofilm.
CC {ECO:0000269|PubMed:12455984, ECO:0000269|PubMed:15673737}.
CC -!- DISRUPTION PHENOTYPE: Leads to exclusive filamentous growth.
CC {ECO:0000269|PubMed:12455984}.
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DR EMBL; CP017623; AOW25708.1; -; Genomic_DNA.
DR RefSeq; XP_719068.2; XM_713975.2.
DR AlphaFoldDB; P0CY34; -.
DR SMR; P0CY34; -.
DR BioGRID; 1222463; 6.
DR IntAct; P0CY34; 1.
DR MINT; P0CY34; -.
DR STRING; 237561.P0CY34; -.
DR PRIDE; P0CY34; -.
DR GeneID; 3639369; -.
DR KEGG; cal:CAALFM_C100060WA; -.
DR CGD; CAL0000186393; TUP1.
DR VEuPathDB; FungiDB:C1_00060W_A; -.
DR eggNOG; KOG0266; Eukaryota.
DR HOGENOM; CLU_000288_57_23_1; -.
DR InParanoid; P0CY34; -.
DR OMA; EHTSVIC; -.
DR OrthoDB; 872177at2759; -.
DR PRO; PR:P0CY34; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0098609; P:cell-cell adhesion; IMP:CGD.
DR GO; GO:0071280; P:cellular response to copper ion; IMP:CGD.
DR GO; GO:0097308; P:cellular response to farnesol; IMP:CGD.
DR GO; GO:0009267; P:cellular response to starvation; IMP:CGD.
DR GO; GO:0044114; P:development of symbiont in host; IMP:CGD.
DR GO; GO:0044409; P:entry into host; IMP:CGD.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0036180; P:filamentous growth of a population of unicellular organisms in response to biotic stimulus; IMP:CGD.
DR GO; GO:0036171; P:filamentous growth of a population of unicellular organisms in response to chemical stimulus; IMP:CGD.
DR GO; GO:0036170; P:filamentous growth of a population of unicellular organisms in response to starvation; IMP:CGD.
DR GO; GO:0030682; P:mitigation of host defenses by symbiont; IMP:CGD.
DR GO; GO:1900429; P:negative regulation of filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0045827; P:negative regulation of isoprenoid metabolic process; IMP:CGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:CGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:CGD.
DR GO; GO:0036166; P:phenotypic switching; IMP:CGD.
DR GO; GO:0009372; P:quorum sensing; IMP:CGD.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001632; Gprotein_B.
DR InterPro; IPR013890; Tscrpt_rep_Tup1_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08581; Tup_N; 1.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00319; GPROTEINB.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Virulence; WD repeat.
FT CHAIN 1..512
FT /note="Transcriptional repressor TUP1"
FT /id="PRO_0000051308"
FT REPEAT 198..238
FT /note="WD 1"
FT REPEAT 253..294
FT /note="WD 2"
FT REPEAT 295..334
FT /note="WD 3"
FT REPEAT 336..375
FT /note="WD 4"
FT REPEAT 385..424
FT /note="WD 5"
FT REPEAT 432..471
FT /note="WD 6"
FT REPEAT 474..511
FT /note="WD 7"
FT REGION 90..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 512 AA; 57603 MW; 2D286A05CDFB69F9 CRC64;
MYPQRTQHQQ RLTELLDAIK TEFDYASNEA SSFKKVQEDY DSKYQQQAAE MQQIRQTVYD
LELAHRKIKE AYEEEILRLK NELDTRDRQM KNGFQQQQQQ QQQQQQQQQQ QQQQIVAPPA
APPAPPTPVT SLSVIDKSQY IVNPTQRANH VKEIPPFLQD LDIAKANPEF KKQHLEYYVL
YNPAFSKDLD IDMVHSLDHS SVVCCVRFSR DGKFIATGCN KTTQVFNVTT GELVAKLIDE
SSNENKDDNT TASGDLYIRS VCFSPDGKLL ATGAEDKLIR IWDLSTKRII KILRGHEQDI
YSLDFFPDGD RLVSGSGDRS VRIWDLRTSQ CSLTLSIEDG VTTVAVSPDG KLIAAGSLDR
TVRVWDSTTG FLVERLDSGN ENGNGHEDSV YSVAFSNNGE QIASGSLDRT VKLWHLEGKS
DKKSTCEVTY IGHKDFVLSV CCTPDNEYIL SGSKDRGVIF WDQASGNPLL MLQGHRNSVI
SVAVSLNSKG TEGIFATGSG DCKARIWKWT KK
