ID   TUPA_PEPAC              Reviewed;         286 AA.
AC   Q93KD6;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Tungstate-binding protein TupA {ECO:0000305};
DE   Flags: Precursor;
GN   Name=tupA {ECO:0000303|PubMed:11292832};
OS   Peptoclostridium acidaminophilum (Eubacterium acidaminophilum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Peptoclostridium.
OX   NCBI_TaxID=1731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=ATCC 49065 / DSM 3953 / al-2;
RX   PubMed=11292832; DOI=10.1074/jbc.m101293200;
RA   Makdessi K., Andreesen J.R., Pich A.;
RT   "Tungstate uptake by a highly specific ABC transporter in Eubacterium
RT   acidaminophilum.";
RL   J. Biol. Chem. 276:24557-24564(2001).
CC   -!- FUNCTION: Part of an ABC transporter complex involved in tungstate
CC       uptake. Specifically binds tungstate. {ECO:0000269|PubMed:11292832}.
CC   -!- SUBUNIT: Monomer (PubMed:11292832). The complex is composed of two ATP-
CC       binding proteins (TupC), two transmembrane proteins (TupB) and a
CC       solute-binding protein (TupA) (Probable). {ECO:0000269|PubMed:11292832,
CC       ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11292832};
CC       Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; AJ291988; CAC40782.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q93KD6; -.
DR   SMR; Q93KD6; -.
DR   TCDB; 3.A.1.6.2; the atp-binding cassette (abc) superfamily.
DR   BRENDA; 7.3.2.6; 2180.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR024370; PBP_domain.
DR   Pfam; PF12849; PBP_like_2; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Signal; Transport;
KW   Tungsten.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           21..286
FT                   /note="Tungstate-binding protein TupA"
FT                   /id="PRO_5004319715"
FT   LIPID           21
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           21
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   286 AA;  30875 MW;  E5B280B2109C18F6 CRC64;
     MKRLLSIITA VMMLALALTG CAAKQSPEGE VEKTQAKGSI ILATTTSTSD SGLLDYLLPE
     FTKDTGIEAK VVAVGTGQAL QMGKDGEADV LLVHSKAAEE EFVAAGDGLE RKDVMYNDFI
     LVGPANDPLK LKQELPNDIV GALKKISEQK FKFISRGDDS GTHKKELALW TEVGITPEGD
     YYVSAGRGMG DVLKMADEMQ AYTIADRGTY LSMKADLGLD IIVEKDTNLF NQYGVIPVNP
     DKNENINAEG AKAFEEWILS EKAQSLIGEY GKEKYGAPLF TPNAAK