ID   TURB_SYNTU              Reviewed;          35 AA.
AC   C0HLN6;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Turgencin-B {ECO:0000303|PubMed:31940927};
OS   Synoicum turgens (Colonial ascidian).
OC   Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Aplousobranchia;
OC   Polyclinidae; Synoicum.
OX   NCBI_TaxID=2697470 {ECO:0000303|PubMed:31940927};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP   DISULFIDE BONDS, OXIDATION AT MET-5 AND MET-9, AND AMIDATION AT GLY-35.
RX   PubMed=31940927; DOI=10.3390/md18010051;
RA   Hansen I.K.O., Isaksson J., Poth A.G., Hansen K.O., Andersen A.J.C.,
RA   Richard C.S.M., Blencke H.M., Stensvaag K., Craik D.J., Haug T.;
RT   "Isolation and Characterization of Antimicrobial Peptides with Unusual
RT   Disulfide Connectivity from the Colonial Ascidian Synoicum turgens.";
RL   Mar. Drugs 18:0-0(2020).
CC   -!- FUNCTION: Has antimicrobial activity against Gram-positive bacteria
CC       (C.glutamicum ATCC 13032 (MIC=1.6 uM) and B.subtilis ATCC 23857
CC       (MIC=1.6 uM)) and Gram-negative bacteria (E.coli ATCC 25922 (MIC=12.5
CC       uM) and P.aeruginosa ATCC 27853 (MIC=25.0 uM)). Displays very low
CC       activity against the Gram-positive bacteria S.aureus ATCC 9144 (MIC>100
CC       uM). {ECO:0000269|PubMed:31940927}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31940927}.
CC   -!- PTM: Oxidation likely reduces antimicrobial activity against Gram-
CC       positive bacteria and Gram-negative bacteria.
CC       {ECO:0000269|PubMed:31940927}.
CC   -!- MASS SPECTROMETRY: Mass=3538.58; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:31940927};
CC   -!- MASS SPECTROMETRY: Mass=3705.79; Method=Electrospray; Note=With
CC       oxidation at Met-5 and Met-9.; Evidence={ECO:0000269|PubMed:31940927};
CC   -!- MISCELLANEOUS: Displays cytotoxic effects on human melanoma cancer cell
CC       line A2058 (IC(50)=4.1 uM) and the human fibroblast cell line MRC-5
CC       (IC(50)=7.5 uM). {ECO:0000269|PubMed:31940927}.
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DR   AlphaFoldDB; C0HLN6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0051715; P:cytolysis in another organism; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Oxidation; Secreted.
FT   PEPTIDE         1..35
FT                   /note="Turgencin-B"
FT                   /id="PRO_0000449796"
FT   MOD_RES         5
FT                   /note="Methionine sulfoxide"
FT                   /evidence="ECO:0000269|PubMed:31940927"
FT   MOD_RES         9
FT                   /note="Methionine sulfoxide"
FT                   /evidence="ECO:0000269|PubMed:31940927"
FT   MOD_RES         35
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000269|PubMed:31940927"
FT   DISULFID        7..31
FT                   /evidence="ECO:0000269|PubMed:31940927"
FT   DISULFID        11..27
FT                   /evidence="ECO:0000269|PubMed:31940927"
FT   DISULFID        16..24
FT                   /evidence="ECO:0000269|PubMed:31940927"
SQ   SEQUENCE   35 AA;  3548 MW;  B59DAEB0C60A2814 CRC64;
     GIKEMLCNMA CAQTVCKKSG GPLCDTCQAA CKALG