ID   TUSA_ECOSM              Reviewed;          81 AA.
AC   B1LID3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur mediator TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur transfer protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000255|HAMAP-Rule:MF_00413};
GN   Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413};
GN   OrderedLocusNames=EcSMS35_3754;
OS   Escherichia coli (strain SMS-3-5 / SECEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=439855;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SMS-3-5 / SECEC;
RX   PubMed=18708504; DOI=10.1128/jb.00661-08;
RA   Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C.,
RA   Ravel J., Stepanauskas R.;
RT   "Insights into the environmental resistance gene pool from the genome
RT   sequence of the multidrug-resistant environmental isolate Escherichia coli
RT   SMS-3-5.";
RL   J. Bacteriol. 190:6779-6794(2008).
CC   -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC       cell. Part of a sulfur-relay system required for 2-thiolation during
CC       synthesis of 2-thiouridine of the modified wobble base 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC       IscS and stimulates its cysteine desulfurase activity. Accepts an
CC       activated sulfur from IscS, which is then transferred to TusD, and thus
CC       determines the direction of sulfur flow from IscS to 2-thiouridine
CC       formation. Also appears to be involved in sulfur transfer for the
CC       biosynthesis of molybdopterin. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- PATHWAY: tRNA modification. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBUNIT: Interacts with IscS. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000970; ACB18708.1; -; Genomic_DNA.
DR   RefSeq; WP_000130623.1; NC_010498.1.
DR   AlphaFoldDB; B1LID3; -.
DR   BMRB; B1LID3; -.
DR   SMR; B1LID3; -.
DR   EnsemblBacteria; ACB18708; ACB18708; EcSMS35_3754.
DR   GeneID; 66506569; -.
DR   KEGG; ecm:EcSMS35_3754; -.
DR   HOGENOM; CLU_165255_5_0_6; -.
DR   OMA; FCQFLGH; -.
DR   Proteomes; UP000007011; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..81
FT                   /note="Sulfur carrier protein TusA"
FT                   /id="PRO_1000199914"
FT   ACT_SITE        19
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00413"
SQ   SEQUENCE   81 AA;  9125 MW;  63B7BDE4816A3274 CRC64;
     MTDLFSSPDH TLDALGLRCP EPVMMVRKTV RNMQPGETLL IIADDPATTR DIPGFCTFME
     HELVAKETDG LPYRYLIRKS G