TUSA_PROMH
ID TUSA_PROMH Reviewed; 84 AA.
AC B4EWZ6;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur mediator TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur transfer protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000255|HAMAP-Rule:MF_00413};
GN Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413}; OrderedLocusNames=PMI3597;
OS Proteus mirabilis (strain HI4320).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Morganellaceae; Proteus.
OX NCBI_TaxID=529507;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HI4320;
RX PubMed=18375554; DOI=10.1128/jb.01981-07;
RA Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S.,
RA Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T.,
RA Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA Rabbinowitsch E., Walker D., Whithead S., Thomson N.R., Rather P.N.,
RA Parkhill J., Mobley H.L.T.;
RT "Complete genome sequence of uropathogenic Proteus mirabilis, a master of
RT both adherence and motility.";
RL J. Bacteriol. 190:4027-4037(2008).
CC -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC cell. Part of a sulfur-relay system required for 2-thiolation during
CC synthesis of 2-thiouridine of the modified wobble base 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC IscS and stimulates its cysteine desulfurase activity. Accepts an
CC activated sulfur from IscS, which is then transferred to TusD, and thus
CC determines the direction of sulfur flow from IscS to 2-thiouridine
CC formation. Also appears to be involved in sulfur transfer for the
CC biosynthesis of molybdopterin. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- PATHWAY: tRNA modification. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SUBUNIT: Interacts with IscS. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC {ECO:0000255|HAMAP-Rule:MF_00413}.
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DR EMBL; AM942759; CAR47038.1; -; Genomic_DNA.
DR RefSeq; WP_004245397.1; NC_010554.1.
DR AlphaFoldDB; B4EWZ6; -.
DR SMR; B4EWZ6; -.
DR STRING; 529507.PMI3597; -.
DR EnsemblBacteria; CAR47038; CAR47038; PMI3597.
DR GeneID; 6802393; -.
DR KEGG; pmr:PMI3597; -.
DR eggNOG; COG0425; Bacteria.
DR HOGENOM; CLU_165255_5_0_6; -.
DR OMA; FCQFLGH; -.
DR Proteomes; UP000008319; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR CDD; cd03423; SirA; 1.
DR Gene3D; 3.30.110.40; -; 1.
DR HAMAP; MF_00413; Thiourid_synth_A; 1.
DR InterPro; IPR022931; Sulphur_carrier_TusA.
DR InterPro; IPR001455; TusA-like.
DR InterPro; IPR036868; TusA-like_sf.
DR Pfam; PF01206; TusA; 1.
DR SUPFAM; SSF64307; SSF64307; 1.
DR PROSITE; PS01148; UPF0033; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; tRNA processing.
FT CHAIN 1..84
FT /note="Sulfur carrier protein TusA"
FT /id="PRO_1000199925"
FT ACT_SITE 19
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00413"
SQ SEQUENCE 84 AA; 9638 MW; 95744A5BCED4B934 CRC64;
MNAQFDDATK TLDTLGLRCP EPVMLVRKTI RNMAQGETLL VIADDPATVR DIPGFCRFME
HDLLQQETQA TPYRYLIRKK EGQL
