ID   TUSA_PSEPK              Reviewed;          80 AA.
AC   Q88L21;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
GN   Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413}; OrderedLocusNames=PP_2116;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: Sulfur carrier protein which probably makes part of a sulfur-
CC       relay system. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE015451; AAN67729.1; -; Genomic_DNA.
DR   RefSeq; NP_744265.1; NC_002947.4.
DR   RefSeq; WP_003248727.1; NC_002947.4.
DR   AlphaFoldDB; Q88L21; -.
DR   SMR; Q88L21; -.
DR   STRING; 160488.PP_2116; -.
DR   EnsemblBacteria; AAN67729; AAN67729; PP_2116.
DR   GeneID; 58529991; -.
DR   GeneID; 66678671; -.
DR   KEGG; ppu:PP_2116; -.
DR   PATRIC; fig|160488.4.peg.2232; -.
DR   eggNOG; COG0425; Bacteria.
DR   HOGENOM; CLU_165255_5_1_6; -.
DR   OMA; FCQFLGH; -.
DR   PhylomeDB; Q88L21; -.
DR   BioCyc; PPUT160488:G1G01-2256-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..80
FT                   /note="Sulfur carrier protein TusA"
FT                   /id="PRO_0000159047"
FT   ACT_SITE        17
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00413"
SQ   SEQUENCE   80 AA;  8996 MW;  481896681E4F925C CRC64;
     MTDFTPDAIL DATGLNCPEP VMMLHQHVRN LAAGGLLKVI ATDPSTRRDI PKFCNFLGHE
     LLQQQEDAGT FLYWIRKKAD