ID   TUSA_SALPK              Reviewed;          81 AA.
AC   B5BHL9;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur mediator TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur transfer protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000255|HAMAP-Rule:MF_00413};
GN   Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413}; OrderedLocusNames=SSPA3200;
OS   Salmonella paratyphi A (strain AKU_12601).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=554290;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AKU_12601;
RX   PubMed=19159446; DOI=10.1186/1471-2164-10-36;
RA   Holt K.E., Thomson N.R., Wain J., Langridge G.C., Hasan R., Bhutta Z.A.,
RA   Quail M.A., Norbertczak H., Walker D., Simmonds M., White B., Bason N.,
RA   Mungall K., Dougan G., Parkhill J.;
RT   "Pseudogene accumulation in the evolutionary histories of Salmonella
RT   enterica serovars Paratyphi A and Typhi.";
RL   BMC Genomics 10:36-36(2009).
CC   -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC       cell. Part of a sulfur-relay system required for 2-thiolation during
CC       synthesis of 2-thiouridine of the modified wobble base 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC       IscS and stimulates its cysteine desulfurase activity. Accepts an
CC       activated sulfur from IscS, which is then transferred to TusD, and thus
CC       determines the direction of sulfur flow from IscS to 2-thiouridine
CC       formation. Also appears to be involved in sulfur transfer for the
CC       biosynthesis of molybdopterin. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- PATHWAY: tRNA modification. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBUNIT: Interacts with IscS. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000255|HAMAP-Rule:MF_00413}.
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DR   EMBL; FM200053; CAR61457.1; -; Genomic_DNA.
DR   RefSeq; WP_001541054.1; NC_011147.1.
DR   AlphaFoldDB; B5BHL9; -.
DR   SMR; B5BHL9; -.
DR   GeneID; 66757902; -.
DR   KEGG; sek:SSPA3200; -.
DR   HOGENOM; CLU_165255_5_0_6; -.
DR   OMA; FCQFLGH; -.
DR   Proteomes; UP000001869; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..81
FT                   /note="Sulfur carrier protein TusA"
FT                   /id="PRO_1000199930"
FT   ACT_SITE        19
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00413"
SQ   SEQUENCE   81 AA;  9193 MW;  48AE92800C7F372B CRC64;
     MSDLFSSPDH TLDALGLRCP EPVMMVRKTV RNMQTGETLL IIADDPATTR DIPGFCTFME
     HDLLAQETEG LPYRYLLRKA H