ID   TUSA_YERPY              Reviewed;          84 AA.
AC   B1JPB6;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur mediator TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=Sulfur transfer protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000255|HAMAP-Rule:MF_00413};
GN   Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413}; OrderedLocusNames=YPK_3986;
OS   Yersinia pseudotuberculosis serotype O:3 (strain YPIII).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=502800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YPIII;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C.,
RA   Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.;
RT   "Complete sequence of Yersinia pseudotuberculosis YPIII.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC       cell. Part of a sulfur-relay system required for 2-thiolation during
CC       synthesis of 2-thiouridine of the modified wobble base 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC       IscS and stimulates its cysteine desulfurase activity. Accepts an
CC       activated sulfur from IscS, which is then transferred to TusD, and thus
CC       determines the direction of sulfur flow from IscS to 2-thiouridine
CC       formation. Also appears to be involved in sulfur transfer for the
CC       biosynthesis of molybdopterin. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- PATHWAY: tRNA modification. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBUNIT: Interacts with IscS. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC       {ECO:0000255|HAMAP-Rule:MF_00413}.
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DR   EMBL; CP000950; ACA70249.1; -; Genomic_DNA.
DR   RefSeq; WP_002215973.1; NZ_CP009792.1.
DR   AlphaFoldDB; B1JPB6; -.
DR   SMR; B1JPB6; -.
DR   EnsemblBacteria; ACA70249; ACA70249; YPK_3986.
DR   GeneID; 66843381; -.
DR   KEGG; ypy:YPK_3986; -.
DR   PATRIC; fig|502800.11.peg.334; -.
DR   OMA; FCQFLGH; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   CDD; cd03423; SirA; 1.
DR   Gene3D; 3.30.110.40; -; 1.
DR   HAMAP; MF_00413; Thiourid_synth_A; 1.
DR   InterPro; IPR022931; Sulphur_carrier_TusA.
DR   InterPro; IPR001455; TusA-like.
DR   InterPro; IPR036868; TusA-like_sf.
DR   Pfam; PF01206; TusA; 1.
DR   SUPFAM; SSF64307; SSF64307; 1.
DR   PROSITE; PS01148; UPF0033; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..84
FT                   /note="Sulfur carrier protein TusA"
FT                   /id="PRO_1000199939"
FT   ACT_SITE        19
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00413"
SQ   SEQUENCE   84 AA;  9536 MW;  D39211DAC090B7D5 CRC64;
     MTDIFANPDK TLDALGLRCP EPVMMVRKTV RHMEEGQTLL IIADDPATTR DIPGFCRFMD
     HQLLAQDTEQ TPYRYLVRKG ITAG