ID   TUSB_ECO27              Reviewed;          95 AA.
AC   B7UK53;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Protein TusB {ECO:0000255|HAMAP-Rule:MF_01564};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein B {ECO:0000255|HAMAP-Rule:MF_01564};
GN   Name=tusB {ECO:0000255|HAMAP-Rule:MF_01564}; OrderedLocusNames=E2348C_3592;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/jb.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R.,
RA   Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A.,
RA   Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC       TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC       TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SIMILARITY: Belongs to the DsrH/TusB family. {ECO:0000255|HAMAP-
CC       Rule:MF_01564}.
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DR   EMBL; FM180568; CAS11140.1; -; Genomic_DNA.
DR   RefSeq; WP_000903377.1; NC_011601.1.
DR   AlphaFoldDB; B7UK53; -.
DR   SMR; B7UK53; -.
DR   EnsemblBacteria; CAS11140; CAS11140; E2348C_3592.
DR   GeneID; 66672777; -.
DR   KEGG; ecg:E2348C_3592; -.
DR   HOGENOM; CLU_166087_2_1_6; -.
DR   OMA; MLHTINK; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   Gene3D; 3.40.1260.10; -; 1.
DR   HAMAP; MF_01564; Thiourid_synth_B; 1.
DR   InterPro; IPR027396; DsrEFH-like.
DR   InterPro; IPR023526; Sulphur_relay_TusB.
DR   InterPro; IPR007215; Sulphur_relay_TusB/DsrH.
DR   PANTHER; PTHR37526; PTHR37526; 1.
DR   Pfam; PF04077; DsrH; 1.
DR   SUPFAM; SSF75169; SSF75169; 1.
DR   TIGRFAMs; TIGR03011; sulf_tusB_dsrH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..95
FT                   /note="Protein TusB"
FT                   /id="PRO_1000185448"
SQ   SEQUENCE   95 AA;  10692 MW;  0EEF7574CDF60608 CRC64;
     MLHTLHRSPW LTDFAALLRL LSEGDELLLL QDGVTAAVDG NRYLESLRNA PIKVYALNED
     LIARGLTGQI SNDIIPIDYT DFVRLTVKHS SQMAW