ID   TUSB_ECOLI              Reviewed;          95 AA.
AC   P45530; Q2M708;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Protein TusB;
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein B;
GN   Name=tusB; Synonyms=yheL; OrderedLocusNames=b3343, JW3305;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=16387657; DOI=10.1016/j.molcel.2005.11.001;
RA   Ikeuchi Y., Shigi N., Kato J., Nishimura A., Suzuki T.;
RT   "Mechanistic insights into sulfur relay by multiple sulfur mediators
RT   involved in thiouridine biosynthesis at tRNA wobble positions.";
RL   Mol. Cell 21:97-108(2006).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), AND SUBUNIT.
RX   PubMed=16472754; DOI=10.1016/j.str.2005.11.009;
RA   Numata T., Fukai S., Ikeuchi Y., Suzuki T., Nureki O.;
RT   "Structural basis for sulfur relay to RNA mediated by heterohexameric
RT   TusBCD complex.";
RL   Structure 14:357-366(2006).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       {ECO:0000269|PubMed:16387657}.
CC   -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC       TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC       TusBCD complex interacts with TusE. {ECO:0000269|PubMed:16387657,
CC       ECO:0000269|PubMed:16472754}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DsrH/TusB family. {ECO:0000305}.
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DR   EMBL; U18997; AAA58140.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76368.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77948.1; -; Genomic_DNA.
DR   PIR; B65128; B65128.
DR   RefSeq; NP_417802.1; NC_000913.3.
DR   RefSeq; WP_000903373.1; NZ_SSZK01000008.1.
DR   PDB; 2D1P; X-ray; 2.15 A; C/F/I=1-95.
DR   PDBsum; 2D1P; -.
DR   AlphaFoldDB; P45530; -.
DR   SMR; P45530; -.
DR   BioGRID; 4261617; 51.
DR   ComplexPortal; CPX-2144; TusBCDE complex.
DR   DIP; DIP-12308N; -.
DR   IntAct; P45530; 3.
DR   STRING; 511145.b3343; -.
DR   jPOST; P45530; -.
DR   PaxDb; P45530; -.
DR   PRIDE; P45530; -.
DR   EnsemblBacteria; AAC76368; AAC76368; b3343.
DR   EnsemblBacteria; BAE77948; BAE77948; BAE77948.
DR   GeneID; 947844; -.
DR   KEGG; ecj:JW3305; -.
DR   KEGG; eco:b3343; -.
DR   PATRIC; fig|1411691.4.peg.3388; -.
DR   EchoBASE; EB2733; -.
DR   eggNOG; COG2168; Bacteria.
DR   HOGENOM; CLU_166087_2_1_6; -.
DR   InParanoid; P45530; -.
DR   OMA; MLHTINK; -.
DR   PhylomeDB; P45530; -.
DR   BioCyc; EcoCyc:G7712-MON; -.
DR   BioCyc; MetaCyc:G7712-MON; -.
DR   EvolutionaryTrace; P45530; -.
DR   PRO; PR:P45530; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:1990228; C:sulfurtransferase complex; IDA:EcoCyc.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IDA:ComplexPortal.
DR   Gene3D; 3.40.1260.10; -; 1.
DR   HAMAP; MF_01564; Thiourid_synth_B; 1.
DR   InterPro; IPR027396; DsrEFH-like.
DR   InterPro; IPR023526; Sulphur_relay_TusB.
DR   InterPro; IPR007215; Sulphur_relay_TusB/DsrH.
DR   PANTHER; PTHR37526; PTHR37526; 1.
DR   Pfam; PF04077; DsrH; 1.
DR   SUPFAM; SSF75169; SSF75169; 1.
DR   TIGRFAMs; TIGR03011; sulf_tusB_dsrH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..95
FT                   /note="Protein TusB"
FT                   /id="PRO_0000169508"
FT   STRAND          2..5
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   HELIX           9..11
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   HELIX           14..19
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   STRAND          26..29
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   HELIX           31..37
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   HELIX           44..48
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   STRAND          50..57
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   HELIX           58..63
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   STRAND          74..77
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   HELIX           79..88
FT                   /evidence="ECO:0007829|PDB:2D1P"
FT   STRAND          92..94
FT                   /evidence="ECO:0007829|PDB:2D1P"
SQ   SEQUENCE   95 AA;  10718 MW;  0EEF7560CCB4D608 CRC64;
     MLHTLHRSPW LTDFAALLRL LSEGDELLLL QDGVTAAVDG NRYLESLRNA PIKVYALNED
     LIARGLTGQI SNDIILIDYT DFVRLTVKHP SQMAW