ID   TUSB_KLEP3              Reviewed;          95 AA.
AC   B5XN84;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Protein TusB {ECO:0000255|HAMAP-Rule:MF_01564};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein B {ECO:0000255|HAMAP-Rule:MF_01564};
GN   Name=tusB {ECO:0000255|HAMAP-Rule:MF_01564}; OrderedLocusNames=KPK_0389;
OS   Klebsiella pneumoniae (strain 342).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=507522;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=342;
RX   PubMed=18654632; DOI=10.1371/journal.pgen.1000141;
RA   Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H.,
RA   Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y.,
RA   Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W.,
RA   Methe B.A.;
RT   "Complete genome sequence of the N2-fixing broad host range endophyte
RT   Klebsiella pneumoniae 342 and virulence predictions verified in mice.";
RL   PLoS Genet. 4:E1000141-E1000141(2008).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC       TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC       TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SIMILARITY: Belongs to the DsrH/TusB family. {ECO:0000255|HAMAP-
CC       Rule:MF_01564}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000964; ACI06575.1; -; Genomic_DNA.
DR   AlphaFoldDB; B5XN84; -.
DR   SMR; B5XN84; -.
DR   EnsemblBacteria; ACI06575; ACI06575; KPK_0389.
DR   KEGG; kpe:KPK_0389; -.
DR   HOGENOM; CLU_166087_2_1_6; -.
DR   OMA; MLHTINK; -.
DR   OrthoDB; 1995110at2; -.
DR   Proteomes; UP000001734; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   Gene3D; 3.40.1260.10; -; 1.
DR   HAMAP; MF_01564; Thiourid_synth_B; 1.
DR   InterPro; IPR027396; DsrEFH-like.
DR   InterPro; IPR023526; Sulphur_relay_TusB.
DR   InterPro; IPR007215; Sulphur_relay_TusB/DsrH.
DR   PANTHER; PTHR37526; PTHR37526; 1.
DR   Pfam; PF04077; DsrH; 1.
DR   SUPFAM; SSF75169; SSF75169; 1.
DR   TIGRFAMs; TIGR03011; sulf_tusB_dsrH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..95
FT                   /note="Protein TusB"
FT                   /id="PRO_1000189841"
SQ   SEQUENCE   95 AA;  10229 MW;  28B0D34BFDE01CD6 CRC64;
     MLHTLSVSPW HADIAAMLRV MDHGDDLLLL SDGVTAAIAG GRFLEILQSA PITLYVLQDD
     VDARGLAGQI ADSVGRVSYT DFVRLTVKHA GQLAW