ID   TUSB_SALDC              Reviewed;          95 AA.
AC   B5FJM3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Protein TusB {ECO:0000255|HAMAP-Rule:MF_01564};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein B {ECO:0000255|HAMAP-Rule:MF_01564};
GN   Name=tusB {ECO:0000255|HAMAP-Rule:MF_01564}; OrderedLocusNames=SeD_A3817;
OS   Salmonella dublin (strain CT_02021853).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=439851;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CT_02021853;
RX   PubMed=21602358; DOI=10.1128/jb.00297-11;
RA   Fricke W.F., Mammel M.K., McDermott P.F., Tartera C., White D.G.,
RA   Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Comparative genomics of 28 Salmonella enterica isolates: evidence for
RT   CRISPR-mediated adaptive sublineage evolution.";
RL   J. Bacteriol. 193:3556-3568(2011).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC       TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC       TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SIMILARITY: Belongs to the DsrH/TusB family. {ECO:0000255|HAMAP-
CC       Rule:MF_01564}.
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DR   EMBL; CP001144; ACH77857.1; -; Genomic_DNA.
DR   RefSeq; WP_000903399.1; NC_011205.1.
DR   AlphaFoldDB; B5FJM3; -.
DR   SMR; B5FJM3; -.
DR   KEGG; sed:SeD_A3817; -.
DR   HOGENOM; CLU_166087_2_1_6; -.
DR   OMA; MLHTINK; -.
DR   Proteomes; UP000008322; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR   Gene3D; 3.40.1260.10; -; 1.
DR   HAMAP; MF_01564; Thiourid_synth_B; 1.
DR   InterPro; IPR027396; DsrEFH-like.
DR   InterPro; IPR023526; Sulphur_relay_TusB.
DR   InterPro; IPR007215; Sulphur_relay_TusB/DsrH.
DR   PANTHER; PTHR37526; PTHR37526; 1.
DR   Pfam; PF04077; DsrH; 1.
DR   SUPFAM; SSF75169; SSF75169; 1.
DR   TIGRFAMs; TIGR03011; sulf_tusB_dsrH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..95
FT                   /note="Protein TusB"
FT                   /id="PRO_1000189843"
SQ   SEQUENCE   95 AA;  10518 MW;  544E5B6C6481CAD7 CRC64;
     MLHTLPHCAS SVDFPALLRL LKEGDALLLL QDGVTVAIEG NRFLESLRDA PITVYALKED
     IDARGLGGQI SDSVVRVDYT DFVRLTVKYA NQMAW