ID   TUSB_SALTY              Reviewed;          95 AA.
AC   Q8ZLL9;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Protein TusB {ECO:0000255|HAMAP-Rule:MF_01564};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein B {ECO:0000255|HAMAP-Rule:MF_01564};
GN   Name=tusB {ECO:0000255|HAMAP-Rule:MF_01564}; OrderedLocusNames=STM3449;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC       TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC       TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01564}.
CC   -!- SIMILARITY: Belongs to the DsrH/TusB family. {ECO:0000255|HAMAP-
CC       Rule:MF_01564}.
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DR   EMBL; AE006468; AAL22312.1; -; Genomic_DNA.
DR   RefSeq; NP_462353.1; NC_003197.2.
DR   RefSeq; WP_000903400.1; NC_003197.2.
DR   AlphaFoldDB; Q8ZLL9; -.
DR   SMR; Q8ZLL9; -.
DR   STRING; 99287.STM3449; -.
DR   PaxDb; Q8ZLL9; -.
DR   EnsemblBacteria; AAL22312; AAL22312; STM3449.
DR   GeneID; 1254972; -.
DR   KEGG; stm:STM3449; -.
DR   PATRIC; fig|99287.12.peg.3646; -.
DR   HOGENOM; CLU_166087_2_1_6; -.
DR   OMA; MLHTINK; -.
DR   PhylomeDB; Q8ZLL9; -.
DR   BioCyc; SENT99287:STM3449-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:1990228; C:sulfurtransferase complex; IBA:GO_Central.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IBA:GO_Central.
DR   Gene3D; 3.40.1260.10; -; 1.
DR   HAMAP; MF_01564; Thiourid_synth_B; 1.
DR   InterPro; IPR027396; DsrEFH-like.
DR   InterPro; IPR023526; Sulphur_relay_TusB.
DR   InterPro; IPR007215; Sulphur_relay_TusB/DsrH.
DR   PANTHER; PTHR37526; PTHR37526; 1.
DR   Pfam; PF04077; DsrH; 1.
DR   SUPFAM; SSF75169; SSF75169; 1.
DR   TIGRFAMs; TIGR03011; sulf_tusB_dsrH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..95
FT                   /note="Protein TusB"
FT                   /id="PRO_0000234519"
SQ   SEQUENCE   95 AA;  10532 MW;  544E5B6C6481CB92 CRC64;
     MLHTLPHCAS SVDFPALLRL LKEGDALLLL QDGVTVAIEG NRFLESLRDA PITVYALKED
     IDARGLGGQI SDSVVRVDYT EFVRLTVKYA NQMAW