TUSC2_HUMAN
ID TUSC2_HUMAN Reviewed; 110 AA.
AC O75896; B2R4Y9;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Tumor suppressor candidate 2;
DE AltName: Full=Fusion 1 protein;
DE Short=Fus-1 protein;
DE AltName: Full=PDGFA-associated protein 2;
GN Name=TUSC2; Synonyms=C3orf11, FUS1, LGCC, PDAP2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11593436; DOI=10.1038/sj.onc.1204832;
RA Kondo M., Ji L., Kamibayashi C., Tomizawa Y., Randle D., Sekido Y.,
RA Yokota J., Kashuba V., Zabarovsky E., Kuzmin I., Lerman M., Roth J.,
RA Minna J.D.;
RT "Overexpression of candidate tumor suppressor gene FUS1 isolated from the
RT 3p21.3 homozygous deletion region leads to G1 arrest and growth inhibition
RT of lung cancer cells.";
RL Oncogene 20:6258-6262(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Subthalamic nucleus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP MYRISTOYLATION AT GLY-2.
RX PubMed=15126327; DOI=10.1158/0008-5472.can-03-3702;
RA Uno F., Sasaki J., Nishizaki M., Carboni G., Xu K., Atkinson E.N.,
RA Kondo M., Minna J.D., Roth J.A., Ji L.;
RT "Myristoylation of the fus1 protein is required for tumor suppression in
RT human lung cancer cells.";
RL Cancer Res. 64:2969-2976(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: May function as a tumor suppressor, inhibiting colony
CC formation, causing G1 arrest and ultimately inducing apoptosis in
CC homozygous 3p21.3 120-kb region-deficient cells.
CC -!- INTERACTION:
CC O75896; Q5TD97: FHL5; NbExp=3; IntAct=EBI-1052725, EBI-750641;
CC O75896; Q99750: MDFI; NbExp=7; IntAct=EBI-1052725, EBI-724076;
CC O75896; Q93062: RBPMS; NbExp=4; IntAct=EBI-1052725, EBI-740322;
CC -!- TISSUE SPECIFICITY: Strong expression in heart, lung, skeletal muscle,
CC kidney, and pancreas, followed by brain and liver, lowest levels in
CC placenta.
CC -!- PTM: Myristoylation is required for tumor suppressor activity.
CC {ECO:0000269|PubMed:15126327}.
CC -!- SIMILARITY: Belongs to the TUSC2 family. {ECO:0000305}.
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DR EMBL; AF055479; AAC35497.1; -; mRNA.
DR EMBL; AK311998; BAG34936.1; -; mRNA.
DR EMBL; CH471055; EAW65097.1; -; Genomic_DNA.
DR EMBL; BC023976; AAH23976.1; -; mRNA.
DR CCDS; CCDS2819.1; -.
DR RefSeq; NP_009206.1; NM_007275.2.
DR AlphaFoldDB; O75896; -.
DR BioGRID; 116462; 85.
DR IntAct; O75896; 6.
DR STRING; 9606.ENSP00000232496; -.
DR iPTMnet; O75896; -.
DR PhosphoSitePlus; O75896; -.
DR BioMuta; TUSC2; -.
DR EPD; O75896; -.
DR jPOST; O75896; -.
DR MassIVE; O75896; -.
DR MaxQB; O75896; -.
DR PaxDb; O75896; -.
DR PeptideAtlas; O75896; -.
DR PRIDE; O75896; -.
DR ProteomicsDB; 50250; -.
DR Antibodypedia; 30895; 202 antibodies from 30 providers.
DR DNASU; 11334; -.
DR Ensembl; ENST00000232496.5; ENSP00000232496.4; ENSG00000114383.10.
DR GeneID; 11334; -.
DR KEGG; hsa:11334; -.
DR MANE-Select; ENST00000232496.5; ENSP00000232496.4; NM_007275.3; NP_009206.1.
DR UCSC; uc003czy.2; human.
DR CTD; 11334; -.
DR DisGeNET; 11334; -.
DR GeneCards; TUSC2; -.
DR HGNC; HGNC:17034; TUSC2.
DR HPA; ENSG00000114383; Low tissue specificity.
DR MIM; 607052; gene.
DR neXtProt; NX_O75896; -.
DR OpenTargets; ENSG00000114383; -.
DR PharmGKB; PA134968273; -.
DR VEuPathDB; HostDB:ENSG00000114383; -.
DR eggNOG; ENOG502S21H; Eukaryota.
DR GeneTree; ENSGT00390000008040; -.
DR HOGENOM; CLU_152285_0_0_1; -.
DR InParanoid; O75896; -.
DR OMA; WPFSAPA; -.
DR OrthoDB; 1596343at2759; -.
DR PhylomeDB; O75896; -.
DR TreeFam; TF314634; -.
DR PathwayCommons; O75896; -.
DR SignaLink; O75896; -.
DR BioGRID-ORCS; 11334; 31 hits in 1077 CRISPR screens.
DR ChiTaRS; TUSC2; human.
DR GeneWiki; TUSC2; -.
DR GenomeRNAi; 11334; -.
DR Pharos; O75896; Tbio.
DR PRO; PR:O75896; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; O75896; protein.
DR Bgee; ENSG00000114383; Expressed in prefrontal cortex and 207 other tissues.
DR ExpressionAtlas; O75896; baseline and differential.
DR Genevisible; O75896; HS.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0048469; P:cell maturation; IEA:Ensembl.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0001779; P:natural killer cell differentiation; IEA:Ensembl.
DR GO; GO:0032700; P:negative regulation of interleukin-17 production; IEA:Ensembl.
DR GO; GO:0070945; P:neutrophil-mediated killing of gram-negative bacterium; IEA:Ensembl.
DR GO; GO:0006909; P:phagocytosis; IEA:Ensembl.
DR GO; GO:0032733; P:positive regulation of interleukin-10 production; IEA:Ensembl.
DR GO; GO:0051881; P:regulation of mitochondrial membrane potential; IBA:GO_Central.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IEA:Ensembl.
DR InterPro; IPR029393; FUS1.
DR PANTHER; PTHR15453; PTHR15453; 1.
DR Pfam; PF15000; TUSC2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Lipoprotein; Myristate; Phosphoprotein; Reference proteome;
KW Tumor suppressor.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..110
FT /note="Tumor suppressor candidate 2"
FT /id="PRO_0000148170"
FT MOD_RES 50
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:24275569"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000269|PubMed:15126327"
SQ SEQUENCE 110 AA; 12074 MW; 9503BD10637C1504 CRC64;
MGASGSKARG LWPFASAAGG GGSEAAGAEQ ALVRPRGRAV PPFVFTRRGS MFYDEDGDLA
HEFYEETIVT KNGQKRAKLR RVHKNLIPQG IVKLDHPRIH VDFPVILYEV
