TUSC_ECO5E
ID TUSC_ECO5E Reviewed; 119 AA.
AC B5YTQ1;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Protein TusC {ECO:0000255|HAMAP-Rule:MF_00389};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein C {ECO:0000255|HAMAP-Rule:MF_00389};
GN Name=tusC {ECO:0000255|HAMAP-Rule:MF_00389};
GN OrderedLocusNames=ECH74115_4653;
OS Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=444450;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EC4115 / EHEC;
RX PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC {ECO:0000255|HAMAP-Rule:MF_00389}.
CC -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_00389}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00389}.
CC -!- SIMILARITY: Belongs to the DsrF/TusC family. {ECO:0000255|HAMAP-
CC Rule:MF_00389}.
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DR EMBL; CP001164; ACI39215.1; -; Genomic_DNA.
DR RefSeq; WP_000820735.1; NC_011353.1.
DR AlphaFoldDB; B5YTQ1; -.
DR SMR; B5YTQ1; -.
DR KEGG; ecf:ECH74115_4653; -.
DR HOGENOM; CLU_155943_1_0_6; -.
DR OMA; LEMILIM; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1260.10; -; 1.
DR HAMAP; MF_00389; Thiourid_synth_C; 1.
DR InterPro; IPR027396; DsrEFH-like.
DR InterPro; IPR003787; Sulphur_relay_DsrE/F-like.
DR InterPro; IPR037450; Sulphur_relay_TusC.
DR InterPro; IPR017462; Sulphur_relay_TusC/DsrF.
DR PANTHER; PTHR38780; PTHR38780; 1.
DR Pfam; PF02635; DrsE; 1.
DR SUPFAM; SSF75169; SSF75169; 1.
DR TIGRFAMs; TIGR03010; sulf_tusC_dsrF; 1.
PE 3: Inferred from homology;
KW Cytoplasm; tRNA processing.
FT CHAIN 1..119
FT /note="Protein TusC"
FT /id="PRO_1000122835"
SQ SEQUENCE 119 AA; 13145 MW; BC3FE465EA31A804 CRC64;
MKRIAFVFST VPHGTAAGRE GLDALLATSA LTDELAVFFI ADGVFQLLPG QKPDAVLARD
YIATFKLLDL YDIEQCWVCA ASLRERGLDP QTPFVVEATP LEADALRREL ANYDVILRF