ID   TUSC_ECOL6              Reviewed;         119 AA.
AC   Q8FCY2;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Protein TusC {ECO:0000255|HAMAP-Rule:MF_00389};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein C {ECO:0000255|HAMAP-Rule:MF_00389};
GN   Name=tusC {ECO:0000255|HAMAP-Rule:MF_00389}; OrderedLocusNames=c4118;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       {ECO:0000255|HAMAP-Rule:MF_00389}.
CC   -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC       TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC       TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_00389}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00389}.
CC   -!- SIMILARITY: Belongs to the DsrF/TusC family. {ECO:0000255|HAMAP-
CC       Rule:MF_00389}.
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DR   EMBL; AE014075; AAN82556.1; -; Genomic_DNA.
DR   RefSeq; WP_000820734.1; NC_004431.1.
DR   AlphaFoldDB; Q8FCY2; -.
DR   SMR; Q8FCY2; -.
DR   STRING; 199310.c4118; -.
DR   EnsemblBacteria; AAN82556; AAN82556; c4118.
DR   KEGG; ecc:c4118; -.
DR   eggNOG; COG2923; Bacteria.
DR   HOGENOM; CLU_155943_1_0_6; -.
DR   OMA; LEMILIM; -.
DR   BioCyc; ECOL199310:C4118-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1260.10; -; 1.
DR   HAMAP; MF_00389; Thiourid_synth_C; 1.
DR   InterPro; IPR027396; DsrEFH-like.
DR   InterPro; IPR003787; Sulphur_relay_DsrE/F-like.
DR   InterPro; IPR037450; Sulphur_relay_TusC.
DR   InterPro; IPR017462; Sulphur_relay_TusC/DsrF.
DR   PANTHER; PTHR38780; PTHR38780; 1.
DR   Pfam; PF02635; DrsE; 1.
DR   SUPFAM; SSF75169; SSF75169; 1.
DR   TIGRFAMs; TIGR03010; sulf_tusC_dsrF; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..119
FT                   /note="Protein TusC"
FT                   /id="PRO_0000214885"
SQ   SEQUENCE   119 AA;  13073 MW;  FC2EE73F8784E2AF CRC64;
     MKRIAFVFST VPHGTAAGRE GLDALLATSA LTDDLAVFFI ADGVFQLLPG QKPDAVLARD
     YIATFKLLGL YDIEQCWVCA ASLRERGLDP QTPFVVEATP LEADALRREL ANYDVILRF