ID   TUSC_ECOLC              Reviewed;         119 AA.
AC   B1IPV5;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Protein TusC {ECO:0000255|HAMAP-Rule:MF_00389};
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein C {ECO:0000255|HAMAP-Rule:MF_00389};
GN   Name=tusC {ECO:0000255|HAMAP-Rule:MF_00389}; OrderedLocusNames=EcolC_0369;
OS   Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 /
OS   WDCM 00012 / Crooks).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=481805;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Ingram L., Richardson P.;
RT   "Complete sequence of Escherichia coli C str. ATCC 8739.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       {ECO:0000255|HAMAP-Rule:MF_00389}.
CC   -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC       TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC       TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_00389}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00389}.
CC   -!- SIMILARITY: Belongs to the DsrF/TusC family. {ECO:0000255|HAMAP-
CC       Rule:MF_00389}.
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DR   EMBL; CP000946; ACA76047.1; -; Genomic_DNA.
DR   RefSeq; WP_000820714.1; NZ_CP022959.1.
DR   AlphaFoldDB; B1IPV5; -.
DR   SMR; B1IPV5; -.
DR   GeneID; 58460168; -.
DR   KEGG; ecl:EcolC_0369; -.
DR   HOGENOM; CLU_155943_1_0_6; -.
DR   OMA; LEMILIM; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1260.10; -; 1.
DR   HAMAP; MF_00389; Thiourid_synth_C; 1.
DR   InterPro; IPR027396; DsrEFH-like.
DR   InterPro; IPR003787; Sulphur_relay_DsrE/F-like.
DR   InterPro; IPR037450; Sulphur_relay_TusC.
DR   InterPro; IPR017462; Sulphur_relay_TusC/DsrF.
DR   PANTHER; PTHR38780; PTHR38780; 1.
DR   Pfam; PF02635; DrsE; 1.
DR   SUPFAM; SSF75169; SSF75169; 1.
DR   TIGRFAMs; TIGR03010; sulf_tusC_dsrF; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..119
FT                   /note="Protein TusC"
FT                   /id="PRO_1000080231"
SQ   SEQUENCE   119 AA;  13045 MW;  2AF593ADC435CA0D CRC64;
     MKRIAFVFST APHGTAAGRE GLDALLATSA LTDDLAVFFI ADGVFQLLPG QKPDAVLARD
     YIATFKLLGL YDIEQCWVCA ASLRERGLDP QTPFVVEATP LEADALRREL ANYDVILRF