TUSC_ECOLI
ID TUSC_ECOLI Reviewed; 119 AA.
AC P45531; Q2M709;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Protein TusC;
DE AltName: Full=tRNA 2-thiouridine synthesizing protein C;
GN Name=tusC; Synonyms=yheM; OrderedLocusNames=b3344, JW3306;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=16387657; DOI=10.1016/j.molcel.2005.11.001;
RA Ikeuchi Y., Shigi N., Kato J., Nishimura A., Suzuki T.;
RT "Mechanistic insights into sulfur relay by multiple sulfur mediators
RT involved in thiouridine biosynthesis at tRNA wobble positions.";
RL Mol. Cell 21:97-108(2006).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), MUTAGENESIS OF CYS-76 AND CYS-79,
RP AND SUBUNIT.
RX PubMed=16472754; DOI=10.1016/j.str.2005.11.009;
RA Numata T., Fukai S., Ikeuchi Y., Suzuki T., Nureki O.;
RT "Structural basis for sulfur relay to RNA mediated by heterohexameric
RT TusBCD complex.";
RL Structure 14:357-366(2006).
CC -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC {ECO:0000269|PubMed:16387657}.
CC -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC TusBCD complex interacts with TusE. {ECO:0000269|PubMed:16387657,
CC ECO:0000269|PubMed:16472754}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DsrF/TusC family. {ECO:0000305}.
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DR EMBL; U18997; AAA58141.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76369.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77947.1; -; Genomic_DNA.
DR PIR; C65128; C65128.
DR RefSeq; NP_417803.1; NC_000913.3.
DR RefSeq; WP_000820714.1; NZ_SSZK01000008.1.
DR PDB; 2D1P; X-ray; 2.15 A; B/E/H=1-119.
DR PDBsum; 2D1P; -.
DR AlphaFoldDB; P45531; -.
DR SMR; P45531; -.
DR BioGRID; 4262468; 28.
DR ComplexPortal; CPX-2144; TusBCDE complex.
DR DIP; DIP-12309N; -.
DR IntAct; P45531; 7.
DR STRING; 511145.b3344; -.
DR PaxDb; P45531; -.
DR PRIDE; P45531; -.
DR EnsemblBacteria; AAC76369; AAC76369; b3344.
DR EnsemblBacteria; BAE77947; BAE77947; BAE77947.
DR GeneID; 58460168; -.
DR GeneID; 947853; -.
DR KEGG; ecj:JW3306; -.
DR KEGG; eco:b3344; -.
DR PATRIC; fig|1411691.4.peg.3387; -.
DR EchoBASE; EB2734; -.
DR eggNOG; COG2923; Bacteria.
DR HOGENOM; CLU_155943_1_0_6; -.
DR InParanoid; P45531; -.
DR OMA; LEMILIM; -.
DR PhylomeDB; P45531; -.
DR BioCyc; EcoCyc:G7713-MON; -.
DR BioCyc; MetaCyc:G7713-MON; -.
DR EvolutionaryTrace; P45531; -.
DR PRO; PR:P45531; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1990228; C:sulfurtransferase complex; IDA:EcoCyc.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IDA:ComplexPortal.
DR Gene3D; 3.40.1260.10; -; 1.
DR HAMAP; MF_00389; Thiourid_synth_C; 1.
DR InterPro; IPR027396; DsrEFH-like.
DR InterPro; IPR003787; Sulphur_relay_DsrE/F-like.
DR InterPro; IPR037450; Sulphur_relay_TusC.
DR InterPro; IPR017462; Sulphur_relay_TusC/DsrF.
DR PANTHER; PTHR38780; PTHR38780; 1.
DR Pfam; PF02635; DrsE; 1.
DR SUPFAM; SSF75169; SSF75169; 1.
DR TIGRFAMs; TIGR03010; sulf_tusC_dsrF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; tRNA processing.
FT CHAIN 1..119
FT /note="Protein TusC"
FT /id="PRO_0000214883"
FT MUTAGEN 76
FT /note="C->S: No effect."
FT /evidence="ECO:0000269|PubMed:16472754"
FT MUTAGEN 79
FT /note="C->S: No effect."
FT /evidence="ECO:0000269|PubMed:16472754"
FT STRAND 4..8
FT /evidence="ECO:0007829|PDB:2D1P"
FT TURN 12..14
FT /evidence="ECO:0007829|PDB:2D1P"
FT HELIX 17..29
FT /evidence="ECO:0007829|PDB:2D1P"
FT STRAND 35..39
FT /evidence="ECO:0007829|PDB:2D1P"
FT HELIX 41..47
FT /evidence="ECO:0007829|PDB:2D1P"
FT HELIX 53..56
FT /evidence="ECO:0007829|PDB:2D1P"
FT HELIX 62..65
FT /evidence="ECO:0007829|PDB:2D1P"
FT HELIX 66..70
FT /evidence="ECO:0007829|PDB:2D1P"
FT STRAND 76..79
FT /evidence="ECO:0007829|PDB:2D1P"
FT HELIX 80..85
FT /evidence="ECO:0007829|PDB:2D1P"
FT STRAND 99..101
FT /evidence="ECO:0007829|PDB:2D1P"
FT HELIX 103..110
FT /evidence="ECO:0007829|PDB:2D1P"
FT STRAND 114..119
FT /evidence="ECO:0007829|PDB:2D1P"
SQ SEQUENCE 119 AA; 13045 MW; 2AF593ADC435CA0D CRC64;
MKRIAFVFST APHGTAAGRE GLDALLATSA LTDDLAVFFI ADGVFQLLPG QKPDAVLARD
YIATFKLLGL YDIEQCWVCA ASLRERGLDP QTPFVVEATP LEADALRREL ANYDVILRF
