TUSD_YERPN
ID TUSD_YERPN Reviewed; 131 AA.
AC Q1CCT3; D1Q2N2;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Sulfurtransferase TusD {ECO:0000255|HAMAP-Rule:MF_00390};
DE EC=2.8.1.- {ECO:0000255|HAMAP-Rule:MF_00390};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein D {ECO:0000255|HAMAP-Rule:MF_00390};
GN Name=tusD {ECO:0000255|HAMAP-Rule:MF_00390}; OrderedLocusNames=YPN_3870;
GN ORFNames=YP516_4395;
OS Yersinia pestis bv. Antiqua (strain Nepal516).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=377628;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nepal516;
RX PubMed=16740952; DOI=10.1128/jb.00124-06;
RA Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M.,
RA Worsham P., Chu M.C., Andersen G.L.;
RT "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516:
RT evidence of gene reduction in an emerging pathogen.";
RL J. Bacteriol. 188:4453-4463(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nepal516;
RA Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L.,
RA Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R.,
RA Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.;
RT "Yersinia pestis Nepal516A whole genome shotgun sequencing project.";
RL Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC Accepts sulfur from TusA and transfers it in turn to TusE.
CC {ECO:0000255|HAMAP-Rule:MF_00390}.
CC -!- SUBUNIT: Heterohexamer, formed by a dimer of trimers. The hexameric
CC TusBCD complex contains 2 copies each of TusB, TusC and TusD. The
CC TusBCD complex interacts with TusE. {ECO:0000255|HAMAP-Rule:MF_00390}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00390}.
CC -!- SIMILARITY: Belongs to the DsrE/TusD family. {ECO:0000255|HAMAP-
CC Rule:MF_00390}.
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DR EMBL; CP000305; ABG20197.1; -; Genomic_DNA.
DR EMBL; ACNQ01000019; EEO74785.1; -; Genomic_DNA.
DR RefSeq; WP_002212320.1; NZ_ACNQ01000019.1.
DR AlphaFoldDB; Q1CCT3; -.
DR SMR; Q1CCT3; -.
DR EnsemblBacteria; ABG20197; ABG20197; YPN_3870.
DR GeneID; 66843868; -.
DR KEGG; ypn:YPN_3870; -.
DR HOGENOM; CLU_132095_0_0_6; -.
DR OMA; PQDDRNI; -.
DR Proteomes; UP000008936; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1260.10; -; 1.
DR HAMAP; MF_00390; Thiourid_synth_D; 1.
DR InterPro; IPR027396; DsrEFH-like.
DR InterPro; IPR003787; Sulphur_relay_DsrE/F-like.
DR InterPro; IPR017463; Sulphur_relay_TusD/DsrE.
DR Pfam; PF02635; DrsE; 1.
DR SUPFAM; SSF75169; SSF75169; 1.
DR TIGRFAMs; TIGR03012; sulf_tusD_dsrE; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Transferase; tRNA processing.
FT CHAIN 1..131
FT /note="Sulfurtransferase TusD"
FT /id="PRO_1000013259"
FT ACT_SITE 81
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00390"
SQ SEQUENCE 131 AA; 13959 MW; 61B5E3F90DA0D456 CRC64;
MSALKYCLLV TGPAYGTQQA SSAYQFAQAV VGAGHHLVSI FFYREGVLNA NQLTAPASDE
FDLVRAWQQL AAEQAVTLNV CVAAALRRGI TDQHEAEQLN LAAANLQPGF TLSGLGALAE
ATLTCDRMVQ F