C561_ECOLI
ID C561_ECOLI Reviewed; 176 AA.
AC P0ABE5; P08732; P76095;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Cytochrome b561;
DE AltName: Full=Cytochrome b-561;
GN Name=cybB; OrderedLocusNames=b1418, JW5224;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2836696; DOI=10.1007/bf00322437;
RA Nakamura H., Murakami H., Yamato I., Anraku Y.;
RT "Nucleotide sequence of the cybB gene encoding cytochrome b561 in
RT Escherichia coli K12.";
RL Mol. Gen. Genet. 212:1-5(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP 1-28 AND 165-176.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CHARACTERIZATION.
RX PubMed=3510204; DOI=10.1016/s0021-9258(17)36126-4;
RA Murakami H., Kita K., Anraku Y.;
RT "Purification and properties of a diheme cytochrome b561 of the Escherichia
RT coli respiratory chain.";
RL J. Biol. Chem. 261:548-551(1986).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: B-type di-heme cytochrome with a major alpha-absorption peak
CC at 561 nm and a minor peak at 555 nm.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Note=Binds 2 heme B (iron-protoporphyrin IX) groups per molecule.;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Redox potential:
CC E(0) is +20 mV.;
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the cytochrome b561 family. {ECO:0000305}.
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DR EMBL; X07569; CAA30454.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74500.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15039.2; -; Genomic_DNA.
DR RefSeq; NP_415935.2; NC_000913.3.
DR RefSeq; WP_000428998.1; NZ_SSZK01000021.1.
DR PDB; 5OC0; X-ray; 1.97 A; A=1-176.
DR PDBsum; 5OC0; -.
DR AlphaFoldDB; P0ABE5; -.
DR SMR; P0ABE5; -.
DR BioGRID; 4262882; 9.
DR STRING; 511145.b1418; -.
DR jPOST; P0ABE5; -.
DR PaxDb; P0ABE5; -.
DR PRIDE; P0ABE5; -.
DR DNASU; 947241; -.
DR EnsemblBacteria; AAC74500; AAC74500; b1418.
DR EnsemblBacteria; BAA15039; BAA15039; BAA15039.
DR GeneID; 947241; -.
DR KEGG; ecj:JW5224; -.
DR KEGG; eco:b1418; -.
DR PATRIC; fig|1411691.4.peg.852; -.
DR EchoBASE; EB0169; -.
DR eggNOG; COG3038; Bacteria.
DR HOGENOM; CLU_095321_3_0_6; -.
DR InParanoid; P0ABE5; -.
DR OMA; FIKGWHE; -.
DR PhylomeDB; P0ABE5; -.
DR BioCyc; EcoCyc:CYTOCHROME-B561-MON; -.
DR BioCyc; MetaCyc:CYTOCHROME-B561-MON; -.
DR PRO; PR:P0ABE5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0009055; F:electron transfer activity; IDA:EcoCyc.
DR GO; GO:0020037; F:heme binding; IDA:EcoCyc.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019430; P:removal of superoxide radicals; IMP:EcoCyc.
DR GO; GO:0022904; P:respiratory electron transport chain; IEA:InterPro.
DR InterPro; IPR011577; Cyt_b561_bac/Ni-Hgenase.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR Pfam; PF01292; Ni_hydr_CYTB; 1.
DR SUPFAM; SSF81342; SSF81342; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Electron transport; Heme;
KW Iron; Membrane; Metal-binding; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..176
FT /note="Cytochrome b561"
FT /id="PRO_0000199971"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..40
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..141
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT BINDING 13
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255"
FT BINDING 45
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255"
FT BINDING 151
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255"
FT BINDING 158
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255"
FT CONFLICT 1..28
FT /note="MENKYSRLQISIHWLVFLLVIAAYCAME -> MLWKINIQGYKSAFTGWSFY
FT WLSQRIAQWR (in Ref. 1; CAA30454 and 2; no nucleotide
FT entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 165..176
FT /note="NTLLRMMPRKRS -> HTSTHDAA (in Ref. 1; CAA30454 and 2;
FT no nucleotide entry)"
FT /evidence="ECO:0000305"
FT HELIX 7..28
FT /evidence="ECO:0007829|PDB:5OC0"
FT HELIX 30..32
FT /evidence="ECO:0007829|PDB:5OC0"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:5OC0"
FT HELIX 38..65
FT /evidence="ECO:0007829|PDB:5OC0"
FT HELIX 77..107
FT /evidence="ECO:0007829|PDB:5OC0"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:5OC0"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:5OC0"
FT HELIX 127..160
FT /evidence="ECO:0007829|PDB:5OC0"
FT HELIX 168..170
FT /evidence="ECO:0007829|PDB:5OC0"
FT STRAND 171..173
FT /evidence="ECO:0007829|PDB:5OC0"
SQ SEQUENCE 176 AA; 20286 MW; F017CB35D4F8853F CRC64;
MENKYSRLQI SIHWLVFLLV IAAYCAMEFR GFFPRSDRPL INMIHVSCGI SILVLMVVRL
LLRLKYPTPP IIPKPKPMMT GLAHLGHLVI YLLFIALPVI GLVMMYNRGN PWFAFGLTMP
YASEANFERV DSLKSWHETL ANLGYFVIGL HAAAALAHHY FWKDNTLLRM MPRKRS
