ID   TUSE_BUCAI              Reviewed;         114 AA.
AC   P57539;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Sulfurtransferase TusE;
DE            EC=2.8.1.-;
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein E;
GN   Name=tusE; OrderedLocusNames=BU467;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       Could accept sulfur from TusD (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the TusBCD complex. Interacts with MnmA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DsrC/TusE family. {ECO:0000305}.
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DR   EMBL; BA000003; BAB13164.1; -; Genomic_DNA.
DR   RefSeq; NP_240278.1; NC_002528.1.
DR   RefSeq; WP_009874419.1; NC_002528.1.
DR   AlphaFoldDB; P57539; -.
DR   SMR; P57539; -.
DR   STRING; 107806.10039130; -.
DR   EnsemblBacteria; BAB13164; BAB13164; BAB13164.
DR   KEGG; buc:BU467; -.
DR   PATRIC; fig|107806.10.peg.476; -.
DR   eggNOG; COG2920; Bacteria.
DR   HOGENOM; CLU_153199_0_0_6; -.
DR   OMA; EYNTSPA; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.370; -; 1.
DR   Gene3D; 3.30.1420.10; -; 1.
DR   InterPro; IPR042072; DsrC-like_C.
DR   InterPro; IPR025526; DsrC-like_dom_sf.
DR   InterPro; IPR043163; DsrC-like_N.
DR   InterPro; IPR007453; DsrC/TusE.
DR   PANTHER; PTHR37010; PTHR37010; 1.
DR   Pfam; PF04358; DsrC; 1.
DR   PIRSF; PIRSF006223; DsrC_TusE; 1.
DR   SUPFAM; SSF69721; SSF69721; 1.
DR   TIGRFAMs; TIGR03342; dsrC_tusE_dsvC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Transferase; tRNA processing.
FT   CHAIN           1..114
FT                   /note="Sulfurtransferase TusE"
FT                   /id="PRO_0000216256"
FT   ACT_SITE        113
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   114 AA;  13364 MW;  49D217E2DCDF2010 CRC64;
     MNTDNKILYT YENLEKDSEG YLKKTKDWNI KLAEEIAKRE NITLSSDHWK VIIFVRKFYF
     KFNITPSMRM LIKSIQKEIG KSKMNSIYLF KLFPKGPAKQ ASKIAGIPKP VKCL