ID   TUSE_BUCBP              Reviewed;          94 AA.
AC   Q89AA8;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Sulfurtransferase TusE;
DE            EC=2.8.1.-;
DE   AltName: Full=tRNA 2-thiouridine synthesizing protein E;
GN   Name=tusE; OrderedLocusNames=bbp_412;
OS   Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=224915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bp;
RX   PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA   van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA   Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA   Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT   "Reductive genome evolution in Buchnera aphidicola.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC   -!- FUNCTION: Part of a sulfur-relay system required for 2-thiolation of 5-
CC       methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions.
CC       Could accept sulfur from TusD (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the TusBCD complex. Interacts with MnmA (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DsrC/TusE family. {ECO:0000305}.
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DR   EMBL; AE016826; AAO27122.1; -; Genomic_DNA.
DR   RefSeq; WP_011091523.1; NC_004545.1.
DR   AlphaFoldDB; Q89AA8; -.
DR   SMR; Q89AA8; -.
DR   STRING; 224915.bbp_412; -.
DR   EnsemblBacteria; AAO27122; AAO27122; bbp_412.
DR   GeneID; 56470948; -.
DR   KEGG; bab:bbp_412; -.
DR   eggNOG; COG2920; Bacteria.
DR   HOGENOM; CLU_153199_1_0_6; -.
DR   OMA; ELTDKHW; -.
DR   OrthoDB; 1840461at2; -.
DR   Proteomes; UP000000601; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.370; -; 1.
DR   InterPro; IPR042072; DsrC-like_C.
DR   InterPro; IPR025526; DsrC-like_dom_sf.
DR   InterPro; IPR007453; DsrC/TusE.
DR   PANTHER; PTHR37010; PTHR37010; 1.
DR   Pfam; PF04358; DsrC; 1.
DR   PIRSF; PIRSF006223; DsrC_TusE; 1.
DR   SUPFAM; SSF69721; SSF69721; 1.
DR   TIGRFAMs; TIGR03342; dsrC_tusE_dsvC; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Transferase; tRNA processing.
FT   CHAIN           1..94
FT                   /note="Sulfurtransferase TusE"
FT                   /id="PRO_0000216258"
FT   ACT_SITE        93
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   94 AA;  11150 MW;  174D834BD6508DF8 CRC64;
     MNYIPDWNEE LAKKIAKSEF INMTPDHWEI IYIIRNFYLN FNLAPSIRIL IKTLEKMKYN
     KKKCSSRYLL KLFPKNPIKQ ASKIAGVPKT NDCI